Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection
Xenotropic murine leukemia virus-related virus (XMRV) was generated after a recombination event between two endogenous murine leukemia viruses during the production of a prostate cancer cell line. Although the associations of the XMRV infection with human diseases appear unlikely, the XMRV is a retr...
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| creator | Curriu, Marta Carrillo, Jorge Massanella, Marta Garcia, Elisabet Cunyat, Francesc Peña, Ruth Wienberg, Peter Carrato, Cristina Areal, Joan Bofill, Margarita Clotet, Bonaventura Blanco, Julià Cabrera, Cecilia |
| description | Xenotropic murine leukemia virus-related virus (XMRV) was generated after a recombination event between two endogenous murine leukemia viruses during the production of a prostate cancer cell line. Although the associations of the XMRV infection with human diseases appear unlikely, the XMRV is a retrovirus of undefined pathogenic potential, able to replicate in human cells in vitro. Since recent studies using animal models for infection have yielded conflicting results, we set out an ex vivo model for XMRV infection of human tonsillar tissue to determine whether XMRV produced by 22Rv1 cells is able to replicate in human lymphoid organs. Tonsil blocks were infected and infection kinetics and its pathogenic effects were monitored
XMRV, though restricted by APOBEC, enters and integrates into the tissue cells. The infection did not result in changes of T or B-cells, immune activation, nor inflammatory chemokines. Infectious viruses could be recovered from supernatants of infected tonsils by reinfecting DERSE XMRV indicator cell line, although these supernatants could not establish a new infection in fresh tonsil culture, indicating that in our model, the viral replication is controlled by innate antiviral restriction factors.
Overall, the replication-competent retrovirus XMRV, present in a high number of laboratories, is able to infect human lymphoid tissue and produce infectious viruses, even though they were unable to establish a new infection in fresh tonsillar tissue. Hereby, laboratories working with cell lines producing XMRV should have knowledge and understanding of the potential biological biohazardous risks of this virus. |
| doi_str_mv | 10.1371/journal.pone.0037415 |
| format | Article |
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XMRV, though restricted by APOBEC, enters and integrates into the tissue cells. The infection did not result in changes of T or B-cells, immune activation, nor inflammatory chemokines. Infectious viruses could be recovered from supernatants of infected tonsils by reinfecting DERSE XMRV indicator cell line, although these supernatants could not establish a new infection in fresh tonsil culture, indicating that in our model, the viral replication is controlled by innate antiviral restriction factors.
Overall, the replication-competent retrovirus XMRV, present in a high number of laboratories, is able to infect human lymphoid tissue and produce infectious viruses, even though they were unable to establish a new infection in fresh tonsillar tissue. Hereby, laboratories working with cell lines producing XMRV should have knowledge and understanding of the potential biological biohazardous risks of this virus.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0037415</identifier><identifier>PMID: 22616002</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acquired immune deficiency syndrome ; AIDS ; Analysis ; Animal models ; B cells ; Biohazards ; Biology ; Biomarkers ; Cell culture ; Cell Line, Tumor - virology ; Chemokines ; Chemokines - metabolism ; Chronic fatigue syndrome ; Cytokines ; Deoxyribonucleic acid ; DNA ; DNA, Viral - metabolism ; Health aspects ; HIV ; Human immunodeficiency virus ; Humans ; Immune response ; Infection ; Infections ; Infectious diseases ; Inflammation ; Kinetics ; Laboratories ; Leukemia ; Lymphocytes B ; Lymphoid tissue ; Lymphoid Tissue - virology ; Male ; Medical research ; Medicine ; Mouse leukemia complex ; Organs ; Otolaryngology ; Palatine Tonsil - chemistry ; Palatine Tonsil - cytology ; Palatine Tonsil - virology ; Pathogenesis ; Prostate cancer ; Prostatic Neoplasms ; Recombination ; Replication ; Retroviridae Infections - etiology ; RNA, Viral - metabolism ; Signal transduction ; Tonsil ; Virus Replication ; Viruses ; Xenotropic ; Xenotropic murine leukemia virus-related virus</subject><ispartof>PloS one, 2012-05, Vol.7 (5), p.e37415-e37415</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Curriu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Curriu et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-b3dc6ff7b795ec2f13726bdc5ab6b01838a28c6c8e543c588d1c004259fd6ab33</citedby><cites>FETCH-LOGICAL-c593t-b3dc6ff7b795ec2f13726bdc5ab6b01838a28c6c8e543c588d1c004259fd6ab33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353939/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3353939/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22616002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Curriu, Marta</creatorcontrib><creatorcontrib>Carrillo, Jorge</creatorcontrib><creatorcontrib>Massanella, Marta</creatorcontrib><creatorcontrib>Garcia, Elisabet</creatorcontrib><creatorcontrib>Cunyat, Francesc</creatorcontrib><creatorcontrib>Peña, Ruth</creatorcontrib><creatorcontrib>Wienberg, Peter</creatorcontrib><creatorcontrib>Carrato, Cristina</creatorcontrib><creatorcontrib>Areal, Joan</creatorcontrib><creatorcontrib>Bofill, Margarita</creatorcontrib><creatorcontrib>Clotet, Bonaventura</creatorcontrib><creatorcontrib>Blanco, Julià</creatorcontrib><creatorcontrib>Cabrera, Cecilia</creatorcontrib><title>Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Xenotropic murine leukemia virus-related virus (XMRV) was generated after a recombination event between two endogenous murine leukemia viruses during the production of a prostate cancer cell line. Although the associations of the XMRV infection with human diseases appear unlikely, the XMRV is a retrovirus of undefined pathogenic potential, able to replicate in human cells in vitro. Since recent studies using animal models for infection have yielded conflicting results, we set out an ex vivo model for XMRV infection of human tonsillar tissue to determine whether XMRV produced by 22Rv1 cells is able to replicate in human lymphoid organs. Tonsil blocks were infected and infection kinetics and its pathogenic effects were monitored
XMRV, though restricted by APOBEC, enters and integrates into the tissue cells. The infection did not result in changes of T or B-cells, immune activation, nor inflammatory chemokines. Infectious viruses could be recovered from supernatants of infected tonsils by reinfecting DERSE XMRV indicator cell line, although these supernatants could not establish a new infection in fresh tonsil culture, indicating that in our model, the viral replication is controlled by innate antiviral restriction factors.
Overall, the replication-competent retrovirus XMRV, present in a high number of laboratories, is able to infect human lymphoid tissue and produce infectious viruses, even though they were unable to establish a new infection in fresh tonsillar tissue. Hereby, laboratories working with cell lines producing XMRV should have knowledge and understanding of the potential biological biohazardous risks of this virus.</description><subject>Acquired immune deficiency syndrome</subject><subject>AIDS</subject><subject>Analysis</subject><subject>Animal models</subject><subject>B cells</subject><subject>Biohazards</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Cell culture</subject><subject>Cell Line, Tumor - virology</subject><subject>Chemokines</subject><subject>Chemokines - metabolism</subject><subject>Chronic fatigue syndrome</subject><subject>Cytokines</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - metabolism</subject><subject>Health aspects</subject><subject>HIV</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Immune response</subject><subject>Infection</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Inflammation</subject><subject>Kinetics</subject><subject>Laboratories</subject><subject>Leukemia</subject><subject>Lymphocytes B</subject><subject>Lymphoid tissue</subject><subject>Lymphoid Tissue - virology</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Mouse leukemia complex</subject><subject>Organs</subject><subject>Otolaryngology</subject><subject>Palatine Tonsil - chemistry</subject><subject>Palatine Tonsil - cytology</subject><subject>Palatine Tonsil - virology</subject><subject>Pathogenesis</subject><subject>Prostate cancer</subject><subject>Prostatic Neoplasms</subject><subject>Recombination</subject><subject>Replication</subject><subject>Retroviridae Infections - etiology</subject><subject>RNA, Viral - metabolism</subject><subject>Signal transduction</subject><subject>Tonsil</subject><subject>Virus Replication</subject><subject>Viruses</subject><subject>Xenotropic</subject><subject>Xenotropic murine leukemia virus-related virus</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUl1v0zAUjRCIjcI_QGCJl_HQ4o_YTl4mTRODSUNIfIk3y3Gc1sWJgz-qVfvzuGs2rWi6D7avzzn2vfcUxWsEF4hw9GHtkh-kXYxu0AsICS8RfVIco5rgOcOQPH2wPypehLCGkJKKsefFEcYMMQjxcXHzPQWlx2gaY03cAteBVerlAOy2H1fOtCCaEJIGKtmYvG6BvgYbs3EgOnCtBxe9G40CffJm0MDq9Ef3RmaIT2HutZUxc25P4OT3l2-_3gMzdFpF44aXxbNO2qBfTeus-Hnx8cf55_nV10-X52dXc0VrEucNaRXrOt7wmmqFu1w9Zk2rqGxYA1FFKokrxVSlaUkUraoWKQhLTOuuZbIhZFa83euO1gUx9S0IRHBJKaekzIjLPaJ1ci1Gb3rpt8JJI24Tzi-F9NEoqwXrNOW4w4RSXFZt1XCCeH6lIqRpNK-y1un0Wmp63So9RC_tgejhzWBWYuk2ghBK6hyz4mQS8O5v0iGK3uQZWSsH7VL-N0S0ZDUtcYa--w_6eHUTailzAbn9eWhS7UTFWck55BzjHWrxCCpHmweqssk6k_MHhHJPUN6F4HV3XyOCYmfRu8-InUXFZNFMe_OwP_ekO0-Sf-N45Vk</recordid><startdate>20120516</startdate><enddate>20120516</enddate><creator>Curriu, Marta</creator><creator>Carrillo, Jorge</creator><creator>Massanella, Marta</creator><creator>Garcia, Elisabet</creator><creator>Cunyat, Francesc</creator><creator>Peña, Ruth</creator><creator>Wienberg, Peter</creator><creator>Carrato, Cristina</creator><creator>Areal, Joan</creator><creator>Bofill, Margarita</creator><creator>Clotet, Bonaventura</creator><creator>Blanco, Julià</creator><creator>Cabrera, Cecilia</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120516</creationdate><title>Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection</title><author>Curriu, Marta ; Carrillo, Jorge ; Massanella, Marta ; Garcia, Elisabet ; Cunyat, Francesc ; Peña, Ruth ; Wienberg, Peter ; Carrato, Cristina ; Areal, Joan ; Bofill, Margarita ; Clotet, Bonaventura ; Blanco, Julià ; Cabrera, Cecilia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-b3dc6ff7b795ec2f13726bdc5ab6b01838a28c6c8e543c588d1c004259fd6ab33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acquired immune deficiency syndrome</topic><topic>AIDS</topic><topic>Analysis</topic><topic>Animal models</topic><topic>B cells</topic><topic>Biohazards</topic><topic>Biology</topic><topic>Biomarkers</topic><topic>Cell culture</topic><topic>Cell Line, Tumor - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Curriu, Marta</au><au>Carrillo, Jorge</au><au>Massanella, Marta</au><au>Garcia, Elisabet</au><au>Cunyat, Francesc</au><au>Peña, Ruth</au><au>Wienberg, Peter</au><au>Carrato, Cristina</au><au>Areal, Joan</au><au>Bofill, Margarita</au><au>Clotet, Bonaventura</au><au>Blanco, Julià</au><au>Cabrera, Cecilia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-05-16</date><risdate>2012</risdate><volume>7</volume><issue>5</issue><spage>e37415</spage><epage>e37415</epage><pages>e37415-e37415</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Xenotropic murine leukemia virus-related virus (XMRV) was generated after a recombination event between two endogenous murine leukemia viruses during the production of a prostate cancer cell line. Although the associations of the XMRV infection with human diseases appear unlikely, the XMRV is a retrovirus of undefined pathogenic potential, able to replicate in human cells in vitro. Since recent studies using animal models for infection have yielded conflicting results, we set out an ex vivo model for XMRV infection of human tonsillar tissue to determine whether XMRV produced by 22Rv1 cells is able to replicate in human lymphoid organs. Tonsil blocks were infected and infection kinetics and its pathogenic effects were monitored
XMRV, though restricted by APOBEC, enters and integrates into the tissue cells. The infection did not result in changes of T or B-cells, immune activation, nor inflammatory chemokines. Infectious viruses could be recovered from supernatants of infected tonsils by reinfecting DERSE XMRV indicator cell line, although these supernatants could not establish a new infection in fresh tonsil culture, indicating that in our model, the viral replication is controlled by innate antiviral restriction factors.
Overall, the replication-competent retrovirus XMRV, present in a high number of laboratories, is able to infect human lymphoid tissue and produce infectious viruses, even though they were unable to establish a new infection in fresh tonsillar tissue. Hereby, laboratories working with cell lines producing XMRV should have knowledge and understanding of the potential biological biohazardous risks of this virus.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22616002</pmid><doi>10.1371/journal.pone.0037415</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1324557534 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Acquired immune deficiency syndrome AIDS Analysis Animal models B cells Biohazards Biology Biomarkers Cell culture Cell Line, Tumor - virology Chemokines Chemokines - metabolism Chronic fatigue syndrome Cytokines Deoxyribonucleic acid DNA DNA, Viral - metabolism Health aspects HIV Human immunodeficiency virus Humans Immune response Infection Infections Infectious diseases Inflammation Kinetics Laboratories Leukemia Lymphocytes B Lymphoid tissue Lymphoid Tissue - virology Male Medical research Medicine Mouse leukemia complex Organs Otolaryngology Palatine Tonsil - chemistry Palatine Tonsil - cytology Palatine Tonsil - virology Pathogenesis Prostate cancer Prostatic Neoplasms Recombination Replication Retroviridae Infections - etiology RNA, Viral - metabolism Signal transduction Tonsil Virus Replication Viruses Xenotropic Xenotropic murine leukemia virus-related virus |
| title | Susceptibility of human lymphoid tissue cultured ex vivo to xenotropic murine leukemia virus-related virus (XMRV) infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T19%3A54%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Susceptibility%20of%20human%20lymphoid%20tissue%20cultured%20ex%20vivo%20to%20xenotropic%20murine%20leukemia%20virus-related%20virus%20(XMRV)%20infection&rft.jtitle=PloS%20one&rft.au=Curriu,%20Marta&rft.date=2012-05-16&rft.volume=7&rft.issue=5&rft.spage=e37415&rft.epage=e37415&rft.pages=e37415-e37415&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0037415&rft_dat=%3Cgale_plos_%3EA477077224%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1324557534&rft_id=info:pmid/22616002&rft_galeid=A477077224&rft_doaj_id=oai_doaj_org_article_6fe572f2355248d8b7317ab3833bbe78&rfr_iscdi=true |