Sialyl residues modulate LPS-mediated signaling through the Toll-like receptor 4 complex

We previously reported that neuraminidase (NA) pretreatment of human PBMCs markedly increased their cytokine response to lipopolysaccharide (LPS). To study the mechanisms by which this occurs, we transfected HEK293T cells with plasmids encoding TLR4, CD14, and MD2 (three components of the LPS recept...

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Veröffentlicht in:	PloS one 2012-04, Vol.7 (4), p.e32359-e32359
Hauptverfasser:	Feng, Chiguang, Stamatos, Nicholas M, Dragan, Anatoliy I, Medvedev, Andrei, Whitford, Melissa, Zhang, Lei, Song, Chang, Rallabhandi, Prasad, Cole, Leah, Nhu, Quan M, Vogel, Stefanie N, Geddes, Chris D, Cross, Alan S
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Adapter proteins Bacterial infections Biology Blotting, Western CD14 antigen Cell activation Cell Communication Cell culture Cells, Cultured Chemical bonds Clostridium perfringens Clustering Cobalt Coding Cytokines Cytokines - metabolism Dimerization Energy transfer Exo- alpha -sialidase Exo-a-sialidase Fluorescence Fluorescence Resonance Energy Transfer Gene Expression Regulation Glycosylation Humans Immunology Immunoprecipitation Infections Kidney - cytology Kidney - metabolism Lipopolysaccharide Receptors - genetics Lipopolysaccharide Receptors - metabolism Lipopolysaccharides Lipopolysaccharides - pharmacology Luciferase Lymphocyte Antigen 96 - genetics Lymphocyte Antigen 96 - metabolism Medicine Mitogens Monomers Mutation Neuraminidase - genetics Neuraminidase - metabolism NF-kappa B - genetics NF-kappa B - metabolism NF-κB protein Organic acids Plasmids Polysaccharides Pretreatment Real-Time Polymerase Chain Reaction Residues RNA, Messenger - genetics Rodents Sialic acids Sialic Acids - chemistry Sialic Acids - metabolism Signal Transduction Signaling TLR4 protein Toll-Like Receptor 4 - genetics Toll-Like Receptor 4 - metabolism Toll-like receptors Transfection Vaccines
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