Mesofluidic devices for DNA-programmed combinatorial chemistry
Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard micro...
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| Veröffentlicht in: | PloS one 2012-03, Vol.7 (3), p.e32299-e32299 |
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| creator | Weisinger, Rebecca M Marinelli, Robert J Wrenn, S Jarrett Harbury, Pehr B |
| description | Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially available automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384 different building blocks per chemical step. We demonstrate that fluidic routing of DNA populations in the highly parallel format occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two requirements for the high-fidelity translation and efficient in vitro evolution of small molecules. |
| doi_str_mv | 10.1371/journal.pone.0032299 |
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We demonstrate that fluidic routing of DNA populations in the highly parallel format occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two requirements for the high-fidelity translation and efficient in vitro evolution of small molecules.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0032299</identifier><identifier>PMID: 22479318</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Arrays ; Automation ; Biochemistry ; Biology ; Biopolymers ; Blotting, Southern ; Catalysis ; Cellulose ; Chemical synthesis ; Chemistry ; Combinatorial analysis ; Combinatorial chemistry ; Combinatorial Chemistry Techniques - instrumentation ; Combinatorial Chemistry Techniques - methods ; Deoxyribonucleic acid ; DNA ; DNA - genetics ; DNA biosynthesis ; DNA microarrays ; Format ; Gene expression ; Gene Library ; Hybridization ; Ligands ; Medicine ; Membranes ; Nucleic Acid Hybridization ; Permeability ; Proteins ; Reproducibility of Results ; Routing ; Small Molecule Libraries ; Technology application</subject><ispartof>PloS one, 2012-03, Vol.7 (3), p.e32299-e32299</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Weisinger et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Weisinger et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-7ff9a277d8c5e84154bd1251162e604488b4fdccaeb3c682cba124b4f0cc7cb53</citedby><cites>FETCH-LOGICAL-c691t-7ff9a277d8c5e84154bd1251162e604488b4fdccaeb3c682cba124b4f0cc7cb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315586/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315586/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22479318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Romesberg, Floyd</contributor><creatorcontrib>Weisinger, Rebecca M</creatorcontrib><creatorcontrib>Marinelli, Robert J</creatorcontrib><creatorcontrib>Wrenn, S Jarrett</creatorcontrib><creatorcontrib>Harbury, Pehr B</creatorcontrib><title>Mesofluidic devices for DNA-programmed combinatorial chemistry</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful tools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially available automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384 different building blocks per chemical step. We demonstrate that fluidic routing of DNA populations in the highly parallel format occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two requirements for the high-fidelity translation and efficient in vitro evolution of small molecules.</description><subject>Arrays</subject><subject>Automation</subject><subject>Biochemistry</subject><subject>Biology</subject><subject>Biopolymers</subject><subject>Blotting, Southern</subject><subject>Catalysis</subject><subject>Cellulose</subject><subject>Chemical synthesis</subject><subject>Chemistry</subject><subject>Combinatorial analysis</subject><subject>Combinatorial chemistry</subject><subject>Combinatorial Chemistry Techniques - instrumentation</subject><subject>Combinatorial Chemistry Techniques - methods</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA biosynthesis</subject><subject>DNA 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In order to extend these efforts, we present a highly parallel format for DNA-programmed chemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially available automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384 different building blocks per chemical step. We demonstrate that fluidic routing of DNA populations in the highly parallel format occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two requirements for the high-fidelity translation and efficient in vitro evolution of small molecules.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22479318</pmid><doi>10.1371/journal.pone.0032299</doi><tpages>e32299</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arrays Automation Biochemistry Biology Biopolymers Blotting, Southern Catalysis Cellulose Chemical synthesis Chemistry Combinatorial analysis Combinatorial chemistry Combinatorial Chemistry Techniques - instrumentation Combinatorial Chemistry Techniques - methods Deoxyribonucleic acid DNA DNA - genetics DNA biosynthesis DNA microarrays Format Gene expression Gene Library Hybridization Ligands Medicine Membranes Nucleic Acid Hybridization Permeability Proteins Reproducibility of Results Routing Small Molecule Libraries Technology application |
| title | Mesofluidic devices for DNA-programmed combinatorial chemistry |
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