Practical application of methanol-mediated mutualistic symbiosis between Methylobacterium species and a roof greening moss, Racomitrium japonicum
Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R....
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| Veröffentlicht in: | PloS one 2012-03, Vol.7 (3), p.e33800-e33800 |
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| creator | Tani, Akio Takai, Yuichiro Suzukawa, Ikko Akita, Motomu Murase, Haruhiko Kimbara, Kazuhide |
| description | Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production. |
| doi_str_mv | 10.1371/journal.pone.0033800 |
| format | Article |
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Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0033800</identifier><identifier>PMID: 22479445</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Arabidopsis - growth & development ; Arachis hypogaea ; Bacteria ; Biology ; Brassica campestris ; Bryophyta ; Bryophyta - growth & development ; Bryophyta - metabolism ; Bryophytes ; Cell culture ; Deoxyribonucleic acid ; Desiccation ; DNA ; Drinking water ; Drying ; Flowers & plants ; Funaria hygrometrica ; Glycine max ; Greening ; Heat ; Hypotheses ; Liquid culture ; Marchantiales ; Methanol ; Methanol - metabolism ; Methylobacterium - growth & development ; Methylobacterium - metabolism ; Methylotrophic bacteria ; Microorganisms ; Mosses ; Nicotiana - growth & development ; Physiology ; Plant growth ; Plant sciences ; Production capacity ; Promotion ; Pseudomonas fluorescens ; Racomitrium ; Soils ; Species ; Sustainable living ; Symbiosis ; Technology application ; Urban areas ; Yeast</subject><ispartof>PloS one, 2012-03, Vol.7 (3), p.e33800-e33800</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Tani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production.</description><subject>Agriculture</subject><subject>Arabidopsis - growth & development</subject><subject>Arachis hypogaea</subject><subject>Bacteria</subject><subject>Biology</subject><subject>Brassica campestris</subject><subject>Bryophyta</subject><subject>Bryophyta - growth & development</subject><subject>Bryophyta - metabolism</subject><subject>Bryophytes</subject><subject>Cell culture</subject><subject>Deoxyribonucleic acid</subject><subject>Desiccation</subject><subject>DNA</subject><subject>Drinking water</subject><subject>Drying</subject><subject>Flowers & plants</subject><subject>Funaria hygrometrica</subject><subject>Glycine max</subject><subject>Greening</subject><subject>Heat</subject><subject>Hypotheses</subject><subject>Liquid culture</subject><subject>Marchantiales</subject><subject>Methanol</subject><subject>Methanol - metabolism</subject><subject>Methylobacterium - growth & development</subject><subject>Methylobacterium - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tani, Akio</au><au>Takai, Yuichiro</au><au>Suzukawa, Ikko</au><au>Akita, Motomu</au><au>Murase, Haruhiko</au><au>Kimbara, Kazuhide</au><au>Janssen, Paul Jaak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Practical application of methanol-mediated mutualistic symbiosis between Methylobacterium species and a roof greening moss, Racomitrium japonicum</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-03-29</date><risdate>2012</risdate><volume>7</volume><issue>3</issue><spage>e33800</spage><epage>e33800</epage><pages>e33800-e33800</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bryophytes, or mosses, are considered the most maintenance-free materials for roof greening. Racomitrium species are most often used due to their high tolerance to desiccation. Because they grow slowly, a technology for forcing their growth is desired. We succeeded in the efficient production of R. japonicum in liquid culture. The structure of the microbial community is crucial to stabilize the culture. A culture-independent technique revealed that the cultures contain methylotrophic bacteria. Using yeast cells that fluoresce in the presence of methanol, methanol emission from the moss was confirmed, suggesting that it is an important carbon and energy source for the bacteria. We isolated Methylobacterium species from the liquid culture and studied their characteristics. The isolates were able to strongly promote the growth of some mosses including R. japonicum and seed plants, but the plant-microbe combination was important, since growth promotion was not uniform across species. One of the isolates, strain 22A, was cultivated with R. japonicum in liquid culture and in a field experiment, resulting in strong growth promotion. Mutualistic symbiosis can thus be utilized for industrial moss production.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22479445</pmid><doi>10.1371/journal.pone.0033800</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Arabidopsis - growth & development Arachis hypogaea Bacteria Biology Brassica campestris Bryophyta Bryophyta - growth & development Bryophyta - metabolism Bryophytes Cell culture Deoxyribonucleic acid Desiccation DNA Drinking water Drying Flowers & plants Funaria hygrometrica Glycine max Greening Heat Hypotheses Liquid culture Marchantiales Methanol Methanol - metabolism Methylobacterium - growth & development Methylobacterium - metabolism Methylotrophic bacteria Microorganisms Mosses Nicotiana - growth & development Physiology Plant growth Plant sciences Production capacity Promotion Pseudomonas fluorescens Racomitrium Soils Species Sustainable living Symbiosis Technology application Urban areas Yeast |
| title | Practical application of methanol-mediated mutualistic symbiosis between Methylobacterium species and a roof greening moss, Racomitrium japonicum |
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