Functional analysis of general odorant binding protein 2 from the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae)
Odorant binding proteins play a crucial role in transporting semiochemicals across the sensillum lymph to olfactory receptors within the insect antennal sensilla. In this study, the general odorant binding protein 2 gene was cloned from the antennae of Loxostege sticticalis, using reverse transcript...
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| creator | Yin, Jiao Feng, Honglin Sun, Hongyan Xi, Jinghui Cao, Yazhong Li, Kebin |
| description | Odorant binding proteins play a crucial role in transporting semiochemicals across the sensillum lymph to olfactory receptors within the insect antennal sensilla. In this study, the general odorant binding protein 2 gene was cloned from the antennae of Loxostege sticticalis, using reverse transcription PCR and rapid amplification of cDNA ends. Recombinant LstiGOBP2 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR assays indicated that LstiGOBP2 mRNA is expressed mainly in adult antennae, with expression levels differing with developmental age. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the LstiGOBP2 protein has binding affinity to a broad range of odorants. Most importantly, trans-11-tetradecen-1-yl acetate, the pheromone component of Loxostege sticticalis, and trans-2-hexenal and cis-3-hexen-1-ol, the most abundant plant volatiles in essential oils extracted from host plants, had high binding affinities to LstiGOBP2 and elicited strong electrophysiological responses from the antennae of adults. |
| doi_str_mv | 10.1371/journal.pone.0033589 |
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(Lepidoptera: Pyralidae)</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Yin, Jiao ; Feng, Honglin ; Sun, Hongyan ; Xi, Jinghui ; Cao, Yazhong ; Li, Kebin</creator><creatorcontrib>Yin, Jiao ; Feng, Honglin ; Sun, Hongyan ; Xi, Jinghui ; Cao, Yazhong ; Li, Kebin</creatorcontrib><description>Odorant binding proteins play a crucial role in transporting semiochemicals across the sensillum lymph to olfactory receptors within the insect antennal sensilla. In this study, the general odorant binding protein 2 gene was cloned from the antennae of Loxostege sticticalis, using reverse transcription PCR and rapid amplification of cDNA ends. Recombinant LstiGOBP2 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR assays indicated that LstiGOBP2 mRNA is expressed mainly in adult antennae, with expression levels differing with developmental age. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the LstiGOBP2 protein has binding affinity to a broad range of odorants. Most importantly, trans-11-tetradecen-1-yl acetate, the pheromone component of Loxostege sticticalis, and trans-2-hexenal and cis-3-hexen-1-ol, the most abundant plant volatiles in essential oils extracted from host plants, had high binding affinities to LstiGOBP2 and elicited strong electrophysiological responses from the antennae of adults.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0033589</identifier><identifier>PMID: 22479417</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetic acid ; Adults ; Affinity ; Affinity chromatography ; Agriculture ; Allelochemicals ; Amino Acid Sequence ; Amino acids ; Analysis ; Animals ; Antennae ; Antennal sensilla ; Arthropod Antennae - physiology ; Base Sequence ; Binding proteins ; Biology ; Butterflies & moths ; Chromatography ; Complementary DNA ; E coli ; Electrodiagnosis - methods ; Escherichia coli ; Essences and essential oils industry ; Essential oils ; Female ; Fluorescence ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - metabolism ; Functional analysis ; Gene expression ; Gene Expression Profiling ; Host plants ; Insects ; Lepidoptera ; Ligands ; Loxostege sticticalis ; Male ; Molecular Sequence Data ; Moths - classification ; Moths - genetics ; N-Phenyl-naphthylamine ; Odorant receptors ; Odorant-binding protein ; Odorants ; Open Reading Frames ; Pheromones ; Phylogeny ; Plant diseases ; Plant extracts ; Protein Binding ; Proteins ; Pyralidae ; Receptors ; Receptors, Odorant - chemistry ; Receptors, Odorant - genetics ; Receptors, Odorant - isolation & purification ; Receptors, Odorant - metabolism ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - isolation & purification ; Reverse transcription ; Semiochemicals ; Sensilla ; Sequence Alignment ; Transcription (Genetics) ; Volatiles</subject><ispartof>PloS one, 2012-03, Vol.7 (3), p.e33589-e33589</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Yin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Yin et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-1f5fc93035969a04275eb8e395c5287e90db942553c5397a8deb94d780afe8b73</citedby><cites>FETCH-LOGICAL-c691t-1f5fc93035969a04275eb8e395c5287e90db942553c5397a8deb94d780afe8b73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316592/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316592/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22479417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Jiao</creatorcontrib><creatorcontrib>Feng, Honglin</creatorcontrib><creatorcontrib>Sun, Hongyan</creatorcontrib><creatorcontrib>Xi, Jinghui</creatorcontrib><creatorcontrib>Cao, Yazhong</creatorcontrib><creatorcontrib>Li, Kebin</creatorcontrib><title>Functional analysis of general odorant binding protein 2 from the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Odorant binding proteins play a crucial role in transporting semiochemicals across the sensillum lymph to olfactory receptors within the insect antennal sensilla. In this study, the general odorant binding protein 2 gene was cloned from the antennae of Loxostege sticticalis, using reverse transcription PCR and rapid amplification of cDNA ends. Recombinant LstiGOBP2 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR assays indicated that LstiGOBP2 mRNA is expressed mainly in adult antennae, with expression levels differing with developmental age. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the LstiGOBP2 protein has binding affinity to a broad range of odorants. 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methods</subject><subject>Escherichia coli</subject><subject>Essences and essential oils industry</subject><subject>Essential oils</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Functional analysis</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Host plants</subject><subject>Insects</subject><subject>Lepidoptera</subject><subject>Ligands</subject><subject>Loxostege sticticalis</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Moths - classification</subject><subject>Moths - genetics</subject><subject>N-Phenyl-naphthylamine</subject><subject>Odorant receptors</subject><subject>Odorant-binding protein</subject><subject>Odorants</subject><subject>Open Reading Frames</subject><subject>Pheromones</subject><subject>Phylogeny</subject><subject>Plant diseases</subject><subject>Plant extracts</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Pyralidae</subject><subject>Receptors</subject><subject>Receptors, Odorant - chemistry</subject><subject>Receptors, Odorant - genetics</subject><subject>Receptors, Odorant - isolation & purification</subject><subject>Receptors, Odorant - metabolism</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>Reverse transcription</subject><subject>Semiochemicals</subject><subject>Sensilla</subject><subject>Sequence Alignment</subject><subject>Transcription (Genetics)</subject><subject>Volatiles</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1DAUgIso7rr6D0QDC-qCMyZNb_FBWBZXBwZWvL2GNDnpZGiT2qS68-ZPN-N0lxnZByn0kn7nOz2nOUnylOA5oSV5s3bjYEU7752FOcaU5hW7lxwTRtNZkWJ6f-_-KHnk_RrjnFZF8TA5StOsZBkpj5Pfl6OVwbhoQiKeNt545DRqwMIQ15xyg7AB1cYqYxvUDy6AsShFenAdCitAHQjlfqHOhdVrtHTXzgdoAPlgoliKNgqXc_RqCb1Rrg9R-xZ92kS5UQLOHicPtGg9PJmuJ8m3y_dfLz7OllcfFhfny5ksGAkzonMtGcU0ZwUTOEvLHOoKKMtlnlYlMKxqlqV5TmVOWSkqBfFZlRUWGqq6pCfJ8523b53nU_M8JzTNKKNVWURisSOUE2veD6YTw4Y7YfjfBTc0XAyxohY4y2oJuohZKcsg07WsCo1VQZSCohZVdL2bso11B0qCDbHgA-nhG2tWvHE_OaWkyFkaBS8nweB-jOAD74yX0LbCghs9ZyytKKlyFsnTf8i7i5uoRsTvN1a7mFZunfw8KwvGYiHbrPM7qHgo6IyMG02buH4QcHYQEJkA16ERo_d88eXz_7NX3w_ZF3vsCkQbVt6143an-kMw24FycN4PoG97TDDfzslNN_h2Tvg0JzHs2f7_uQ26GQz6B0y2Dr0</recordid><startdate>20120330</startdate><enddate>20120330</enddate><creator>Yin, Jiao</creator><creator>Feng, Honglin</creator><creator>Sun, Hongyan</creator><creator>Xi, Jinghui</creator><creator>Cao, Yazhong</creator><creator>Li, Kebin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120330</creationdate><title>Functional analysis of general odorant binding protein 2 from the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae)</title><author>Yin, Jiao ; Feng, Honglin ; Sun, Hongyan ; Xi, Jinghui ; Cao, Yazhong ; Li, Kebin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-1f5fc93035969a04275eb8e395c5287e90db942553c5397a8deb94d780afe8b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acetic acid</topic><topic>Adults</topic><topic>Affinity</topic><topic>Affinity chromatography</topic><topic>Agriculture</topic><topic>Allelochemicals</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antennae</topic><topic>Antennal sensilla</topic><topic>Arthropod Antennae - physiology</topic><topic>Base Sequence</topic><topic>Binding proteins</topic><topic>Biology</topic><topic>Butterflies & moths</topic><topic>Chromatography</topic><topic>Complementary DNA</topic><topic>E coli</topic><topic>Electrodiagnosis - methods</topic><topic>Escherichia coli</topic><topic>Essences and essential oils industry</topic><topic>Essential oils</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Functional analysis</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Host plants</topic><topic>Insects</topic><topic>Lepidoptera</topic><topic>Ligands</topic><topic>Loxostege sticticalis</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Moths - classification</topic><topic>Moths - genetics</topic><topic>N-Phenyl-naphthylamine</topic><topic>Odorant receptors</topic><topic>Odorant-binding protein</topic><topic>Odorants</topic><topic>Open Reading Frames</topic><topic>Pheromones</topic><topic>Phylogeny</topic><topic>Plant diseases</topic><topic>Plant extracts</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Pyralidae</topic><topic>Receptors</topic><topic>Receptors, Odorant - chemistry</topic><topic>Receptors, Odorant - genetics</topic><topic>Receptors, Odorant - isolation & purification</topic><topic>Receptors, Odorant - metabolism</topic><topic>Recombinant Fusion Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Jiao</au><au>Feng, Honglin</au><au>Sun, Hongyan</au><au>Xi, Jinghui</au><au>Cao, Yazhong</au><au>Li, Kebin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional analysis of general odorant binding protein 2 from the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-03-30</date><risdate>2012</risdate><volume>7</volume><issue>3</issue><spage>e33589</spage><epage>e33589</epage><pages>e33589-e33589</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Odorant binding proteins play a crucial role in transporting semiochemicals across the sensillum lymph to olfactory receptors within the insect antennal sensilla. In this study, the general odorant binding protein 2 gene was cloned from the antennae of Loxostege sticticalis, using reverse transcription PCR and rapid amplification of cDNA ends. Recombinant LstiGOBP2 was expressed in Escherichia coli and purified by Ni ion affinity chromatography. Real-time PCR assays indicated that LstiGOBP2 mRNA is expressed mainly in adult antennae, with expression levels differing with developmental age. Ligand-binding experiments using N-phenyl-naphthylamine (1-NPN) as a fluorescent probe demonstrated that the LstiGOBP2 protein has binding affinity to a broad range of odorants. Most importantly, trans-11-tetradecen-1-yl acetate, the pheromone component of Loxostege sticticalis, and trans-2-hexenal and cis-3-hexen-1-ol, the most abundant plant volatiles in essential oils extracted from host plants, had high binding affinities to LstiGOBP2 and elicited strong electrophysiological responses from the antennae of adults.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22479417</pmid><doi>10.1371/journal.pone.0033589</doi><tpages>e33589</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-03, Vol.7 (3), p.e33589-e33589 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Acetic acid Adults Affinity Affinity chromatography Agriculture Allelochemicals Amino Acid Sequence Amino acids Analysis Animals Antennae Antennal sensilla Arthropod Antennae - physiology Base Sequence Binding proteins Biology Butterflies & moths Chromatography Complementary DNA E coli Electrodiagnosis - methods Escherichia coli Essences and essential oils industry Essential oils Female Fluorescence Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Functional analysis Gene expression Gene Expression Profiling Host plants Insects Lepidoptera Ligands Loxostege sticticalis Male Molecular Sequence Data Moths - classification Moths - genetics N-Phenyl-naphthylamine Odorant receptors Odorant-binding protein Odorants Open Reading Frames Pheromones Phylogeny Plant diseases Plant extracts Protein Binding Proteins Pyralidae Receptors Receptors, Odorant - chemistry Receptors, Odorant - genetics Receptors, Odorant - isolation & purification Receptors, Odorant - metabolism Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Reverse transcription Semiochemicals Sensilla Sequence Alignment Transcription (Genetics) Volatiles |
| title | Functional analysis of general odorant binding protein 2 from the meadow moth, Loxostege sticticalis L. (Lepidoptera: Pyralidae) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T17%3A49%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20analysis%20of%20general%20odorant%20binding%20protein%202%20from%20the%20meadow%20moth,%20Loxostege%20sticticalis%20L.%20(Lepidoptera:%20Pyralidae)&rft.jtitle=PloS%20one&rft.au=Yin,%20Jiao&rft.date=2012-03-30&rft.volume=7&rft.issue=3&rft.spage=e33589&rft.epage=e33589&rft.pages=e33589-e33589&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0033589&rft_dat=%3Cgale_plos_%3EA476993942%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1324393876&rft_id=info:pmid/22479417&rft_galeid=A476993942&rft_doaj_id=oai_doaj_org_article_94bcef6e90394e4fbc86f0d61dde6ba8&rfr_iscdi=true |