Comparison of in vitro- and chorioallantoic membrane (CAM)-culture systems for cryopreserved medulla-contained human ovarian tissue
At present, there are three ways to determine effectively the quality of the cryopreservation procedure using ovarian tissue before the re-implantation treatment: evaluation of follicles after post-thawing xenotransplantation to SCID mouse, in-vitro culture in a large volume of culture medium under...
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| Veröffentlicht in: | PloS one 2012-03, Vol.7 (3), p.e32549-e32549 |
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| creator | Isachenko, Vladimir Mallmann, Peter Petrunkina, Anna M Rahimi, Gohar Nawroth, Frank Hancke, Katharina Felberbaum, Ricardo Genze, Felicitas Damjanoski, Ilija Isachenko, Evgenia |
| description | At present, there are three ways to determine effectively the quality of the cryopreservation procedure using ovarian tissue before the re-implantation treatment: evaluation of follicles after post-thawing xenotransplantation to SCID mouse, in-vitro culture in a large volume of culture medium under constant agitation and culture on embryonic chorio-allantoic membrane within a hen's eggs. The aim of this study was to compare the two methods, culture in vitro and culture on embryonic chorioallantoic membrane (CAM) of cryopreserved human ovarian medulla-contained and medulla-free cortex. Ovarian fragments were divided into small pieces (1.5-2.0×1.0-1.2×0.8-1.5) of two types, cortex with medulla and medulla-free cortex, frozen, thawed and randomly divided into the following four groups. Group 1: medulla-free cortex cultured in vitro for 8 days in large volume of medium with mechanical agitation, Group 2: medulla-containing cortex cultured in vitro, Group 3: medulla-free cortex cultured in CAM-system for 5 days, Group 4: medulla-containing cortex cultured in CAM-system. The efficacy of the tissue culture was evaluated by the development of follicles and by intensiveness of angiogenesis in the tissue (von Willebrand factor and Desmin). For Group 1, 2, 3 and 4, respectively 85%, 85%, 87% and 84% of the follicles were morphologically normal (P>0.1). The immunohistochemical analysis showed that angiogenesis detected by von Willebrand factor was lower in groups 1 and 3 (medulla-free cortex). Neo-vascularisation (by Desmin) was observed only in ovarian tissue of Group 4 (medulla-contained cortex after CAM-culture). It appears that the presence of medulla in ovarian pieces is beneficial for post-thaw development of cryopreserved human ovarian tissue. For medical practice it is recommended for evaluation of post-warming ovarian tissue to use the CAM-system as a valuable alternative to xenotransplantation and for cryopreservation of these tissues to prepare ovarian medulla-contained strips. |
| doi_str_mv | 10.1371/journal.pone.0032549 |
| format | Article |
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The aim of this study was to compare the two methods, culture in vitro and culture on embryonic chorioallantoic membrane (CAM) of cryopreserved human ovarian medulla-contained and medulla-free cortex. Ovarian fragments were divided into small pieces (1.5-2.0×1.0-1.2×0.8-1.5) of two types, cortex with medulla and medulla-free cortex, frozen, thawed and randomly divided into the following four groups. Group 1: medulla-free cortex cultured in vitro for 8 days in large volume of medium with mechanical agitation, Group 2: medulla-containing cortex cultured in vitro, Group 3: medulla-free cortex cultured in CAM-system for 5 days, Group 4: medulla-containing cortex cultured in CAM-system. The efficacy of the tissue culture was evaluated by the development of follicles and by intensiveness of angiogenesis in the tissue (von Willebrand factor and Desmin). For Group 1, 2, 3 and 4, respectively 85%, 85%, 87% and 84% of the follicles were morphologically normal (P>0.1). The immunohistochemical analysis showed that angiogenesis detected by von Willebrand factor was lower in groups 1 and 3 (medulla-free cortex). Neo-vascularisation (by Desmin) was observed only in ovarian tissue of Group 4 (medulla-contained cortex after CAM-culture). It appears that the presence of medulla in ovarian pieces is beneficial for post-thaw development of cryopreserved human ovarian tissue. For medical practice it is recommended for evaluation of post-warming ovarian tissue to use the CAM-system as a valuable alternative to xenotransplantation and for cryopreservation of these tissues to prepare ovarian medulla-contained strips.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0032549</identifier><identifier>PMID: 22479331</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Age ; Agitation ; Angiogenesis ; Animals ; Biology ; Cancer therapies ; Chick Embryo ; Chorioallantoic membrane ; Chorioallantoic Membrane - physiology ; Computer aided manufacturing ; Cryopreservation ; Cryopreservation - methods ; Desmin ; Desmin - analysis ; Eggs ; Embryos ; Evaluation ; Female ; Follicles ; Gene expression ; Gynecology ; Health risk assessment ; Hospitals ; Humans ; Immunohistochemistry ; Implantation ; In vitro methods and tests ; Laboratories ; Medical prognosis ; Medicine ; Medulla ; Medulla oblongata ; Metastasis ; Mice ; Mice, SCID ; Morphology ; Neovascularization, Physiologic ; Obstetrics ; Ovarian Follicle - growth & development ; Ovarian Follicle - metabolism ; Ovarian Follicle - transplantation ; Ovary - blood supply ; Ovary - physiology ; Ovary - transplantation ; Quantitative analysis ; Reproducibility of Results ; Reproductive health ; Smooth muscle ; Thawing ; Time Factors ; Tissue culture ; Tissue Culture Techniques - methods ; Tissues ; Transplantation, Heterologous ; Urology ; Von Willebrand factor ; von Willebrand Factor - analysis ; Xenografts ; Xenotransplantation ; Young Adult</subject><ispartof>PloS one, 2012-03, Vol.7 (3), p.e32549-e32549</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Isachenko et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Isachenko et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-d0de31a3557d3b6e083b1d5f9d54884dbec6f265a8acefc0c1c1d201463d742c3</citedby><cites>FETCH-LOGICAL-c592t-d0de31a3557d3b6e083b1d5f9d54884dbec6f265a8acefc0c1c1d201463d742c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316540/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316540/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22479331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Isachenko, Vladimir</creatorcontrib><creatorcontrib>Mallmann, Peter</creatorcontrib><creatorcontrib>Petrunkina, Anna M</creatorcontrib><creatorcontrib>Rahimi, Gohar</creatorcontrib><creatorcontrib>Nawroth, Frank</creatorcontrib><creatorcontrib>Hancke, Katharina</creatorcontrib><creatorcontrib>Felberbaum, Ricardo</creatorcontrib><creatorcontrib>Genze, Felicitas</creatorcontrib><creatorcontrib>Damjanoski, Ilija</creatorcontrib><creatorcontrib>Isachenko, Evgenia</creatorcontrib><title>Comparison of in vitro- and chorioallantoic membrane (CAM)-culture systems for cryopreserved medulla-contained human ovarian tissue</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>At present, there are three ways to determine effectively the quality of the cryopreservation procedure using ovarian tissue before the re-implantation treatment: evaluation of follicles after post-thawing xenotransplantation to SCID mouse, in-vitro culture in a large volume of culture medium under constant agitation and culture on embryonic chorio-allantoic membrane within a hen's eggs. The aim of this study was to compare the two methods, culture in vitro and culture on embryonic chorioallantoic membrane (CAM) of cryopreserved human ovarian medulla-contained and medulla-free cortex. Ovarian fragments were divided into small pieces (1.5-2.0×1.0-1.2×0.8-1.5) of two types, cortex with medulla and medulla-free cortex, frozen, thawed and randomly divided into the following four groups. Group 1: medulla-free cortex cultured in vitro for 8 days in large volume of medium with mechanical agitation, Group 2: medulla-containing cortex cultured in vitro, Group 3: medulla-free cortex cultured in CAM-system for 5 days, Group 4: medulla-containing cortex cultured in CAM-system. The efficacy of the tissue culture was evaluated by the development of follicles and by intensiveness of angiogenesis in the tissue (von Willebrand factor and Desmin). For Group 1, 2, 3 and 4, respectively 85%, 85%, 87% and 84% of the follicles were morphologically normal (P>0.1). The immunohistochemical analysis showed that angiogenesis detected by von Willebrand factor was lower in groups 1 and 3 (medulla-free cortex). Neo-vascularisation (by Desmin) was observed only in ovarian tissue of Group 4 (medulla-contained cortex after CAM-culture). It appears that the presence of medulla in ovarian pieces is beneficial for post-thaw development of cryopreserved human ovarian tissue. For medical practice it is recommended for evaluation of post-warming ovarian tissue to use the CAM-system as a valuable alternative to xenotransplantation and for cryopreservation of these tissues to prepare ovarian medulla-contained strips.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Age</subject><subject>Agitation</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Biology</subject><subject>Cancer therapies</subject><subject>Chick Embryo</subject><subject>Chorioallantoic membrane</subject><subject>Chorioallantoic Membrane - physiology</subject><subject>Computer aided manufacturing</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Desmin</subject><subject>Desmin - analysis</subject><subject>Eggs</subject><subject>Embryos</subject><subject>Evaluation</subject><subject>Female</subject><subject>Follicles</subject><subject>Gene expression</subject><subject>Gynecology</subject><subject>Health risk assessment</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Implantation</subject><subject>In vitro methods and tests</subject><subject>Laboratories</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>Medulla</subject><subject>Medulla oblongata</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, SCID</subject><subject>Morphology</subject><subject>Neovascularization, Physiologic</subject><subject>Obstetrics</subject><subject>Ovarian Follicle - growth & development</subject><subject>Ovarian Follicle - metabolism</subject><subject>Ovarian Follicle - transplantation</subject><subject>Ovary - blood supply</subject><subject>Ovary - physiology</subject><subject>Ovary - transplantation</subject><subject>Quantitative analysis</subject><subject>Reproducibility of Results</subject><subject>Reproductive health</subject><subject>Smooth muscle</subject><subject>Thawing</subject><subject>Time Factors</subject><subject>Tissue culture</subject><subject>Tissue Culture Techniques - methods</subject><subject>Tissues</subject><subject>Transplantation, Heterologous</subject><subject>Urology</subject><subject>Von Willebrand factor</subject><subject>von Willebrand Factor - analysis</subject><subject>Xenografts</subject><subject>Xenotransplantation</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUkmP0zAUjhCIGQb-AYJIHIBDivckl5GqimWkQVzgbDn2c-sqsYudVOqZP45LM6MpGvlg6_lb3lYUrzFaYFrjT9swRa_6xS54WCBECWftk-ISt5RUgiD69MH7oniR0hYhThshnhcXhLC6pRRfFn9WYdip6FLwZbCl8-XejTFUpfKm1JsQXVB9r_wYnC4HGLqoPJQfVsvvHys99eMUoUyHNMKQShtiqeMh7CIkiHswmWCmzK508KNyPkc206Cy0z5b5nt0KU3wsnhmVZ_g1XxfFb--fP65-lbd_vh6s1reVpq3ZKwMMkCxopzXhnYCUEM7bLhtDWdNw0wHWlgiuGqUBquRxhobgjAT1NSMaHpVvD3p7vqQ5Ny_JDEljLa0btqMuDkhTFBbuYtuUPEgg3LyXyDEtVRxdLoHCY3oKEGGGGFZx3XTKrCKdYI3je2YyVrXs9vU5T5o8GNU_Zno-Y93G7kOe5nnIjhDWeD9LBDD7wnSKAeXNBynAWFKsm1JQ2rOWEa--w_5eHEzaq1y_s7bkG31UVMuWS3aNuNERi0eQeVjYHB5jmBdjp8R2ImgY0gpgr0vESN53NS7ZORxU-W8qZn25mF77kl3q0n_Av4C6TY</recordid><startdate>20120330</startdate><enddate>20120330</enddate><creator>Isachenko, Vladimir</creator><creator>Mallmann, Peter</creator><creator>Petrunkina, Anna M</creator><creator>Rahimi, Gohar</creator><creator>Nawroth, Frank</creator><creator>Hancke, Katharina</creator><creator>Felberbaum, Ricardo</creator><creator>Genze, Felicitas</creator><creator>Damjanoski, Ilija</creator><creator>Isachenko, Evgenia</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120330</creationdate><title>Comparison of in vitro- and chorioallantoic membrane (CAM)-culture systems for cryopreserved medulla-contained human ovarian tissue</title><author>Isachenko, Vladimir ; Mallmann, Peter ; Petrunkina, Anna M ; Rahimi, Gohar ; Nawroth, Frank ; Hancke, Katharina ; Felberbaum, Ricardo ; Genze, Felicitas ; Damjanoski, Ilija ; Isachenko, Evgenia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-d0de31a3557d3b6e083b1d5f9d54884dbec6f265a8acefc0c1c1d201463d742c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Age</topic><topic>Agitation</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Biology</topic><topic>Cancer therapies</topic><topic>Chick Embryo</topic><topic>Chorioallantoic membrane</topic><topic>Chorioallantoic Membrane - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Isachenko, Vladimir</au><au>Mallmann, Peter</au><au>Petrunkina, Anna M</au><au>Rahimi, Gohar</au><au>Nawroth, Frank</au><au>Hancke, Katharina</au><au>Felberbaum, Ricardo</au><au>Genze, Felicitas</au><au>Damjanoski, Ilija</au><au>Isachenko, Evgenia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of in vitro- and chorioallantoic membrane (CAM)-culture systems for cryopreserved medulla-contained human ovarian tissue</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-03-30</date><risdate>2012</risdate><volume>7</volume><issue>3</issue><spage>e32549</spage><epage>e32549</epage><pages>e32549-e32549</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>At present, there are three ways to determine effectively the quality of the cryopreservation procedure using ovarian tissue before the re-implantation treatment: evaluation of follicles after post-thawing xenotransplantation to SCID mouse, in-vitro culture in a large volume of culture medium under constant agitation and culture on embryonic chorio-allantoic membrane within a hen's eggs. The aim of this study was to compare the two methods, culture in vitro and culture on embryonic chorioallantoic membrane (CAM) of cryopreserved human ovarian medulla-contained and medulla-free cortex. Ovarian fragments were divided into small pieces (1.5-2.0×1.0-1.2×0.8-1.5) of two types, cortex with medulla and medulla-free cortex, frozen, thawed and randomly divided into the following four groups. Group 1: medulla-free cortex cultured in vitro for 8 days in large volume of medium with mechanical agitation, Group 2: medulla-containing cortex cultured in vitro, Group 3: medulla-free cortex cultured in CAM-system for 5 days, Group 4: medulla-containing cortex cultured in CAM-system. The efficacy of the tissue culture was evaluated by the development of follicles and by intensiveness of angiogenesis in the tissue (von Willebrand factor and Desmin). For Group 1, 2, 3 and 4, respectively 85%, 85%, 87% and 84% of the follicles were morphologically normal (P>0.1). The immunohistochemical analysis showed that angiogenesis detected by von Willebrand factor was lower in groups 1 and 3 (medulla-free cortex). Neo-vascularisation (by Desmin) was observed only in ovarian tissue of Group 4 (medulla-contained cortex after CAM-culture). It appears that the presence of medulla in ovarian pieces is beneficial for post-thaw development of cryopreserved human ovarian tissue. For medical practice it is recommended for evaluation of post-warming ovarian tissue to use the CAM-system as a valuable alternative to xenotransplantation and for cryopreservation of these tissues to prepare ovarian medulla-contained strips.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22479331</pmid><doi>10.1371/journal.pone.0032549</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-03, Vol.7 (3), p.e32549-e32549 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1324393789 |
| source | Open Access: PubMed Central; MEDLINE; Public Library of Science; DOAJ Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Adolescent Adult Age Agitation Angiogenesis Animals Biology Cancer therapies Chick Embryo Chorioallantoic membrane Chorioallantoic Membrane - physiology Computer aided manufacturing Cryopreservation Cryopreservation - methods Desmin Desmin - analysis Eggs Embryos Evaluation Female Follicles Gene expression Gynecology Health risk assessment Hospitals Humans Immunohistochemistry Implantation In vitro methods and tests Laboratories Medical prognosis Medicine Medulla Medulla oblongata Metastasis Mice Mice, SCID Morphology Neovascularization, Physiologic Obstetrics Ovarian Follicle - growth & development Ovarian Follicle - metabolism Ovarian Follicle - transplantation Ovary - blood supply Ovary - physiology Ovary - transplantation Quantitative analysis Reproducibility of Results Reproductive health Smooth muscle Thawing Time Factors Tissue culture Tissue Culture Techniques - methods Tissues Transplantation, Heterologous Urology Von Willebrand factor von Willebrand Factor - analysis Xenografts Xenotransplantation Young Adult |
| title | Comparison of in vitro- and chorioallantoic membrane (CAM)-culture systems for cryopreserved medulla-contained human ovarian tissue |
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