Polypyrimidine tract binding protein (hnRNP I) is possibly a conserved modulator of miRNA-mediated gene regulation
MiRNAs can regulate gene expression through versatile mechanisms that result in increased or decreased expression of the targeted mRNA and it could effect the expression of thousands of protein in a particular cell. An increasing body of evidence suggest that miRNAs action can be modulated by protei...
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| Veröffentlicht in: | PloS one 2012-03, Vol.7 (3), p.e33144-e33144 |
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| creator | Engels, Bart Jannot, Guillaume Remenyi, Judit Simard, Martin J Hutvagner, György |
| description | MiRNAs can regulate gene expression through versatile mechanisms that result in increased or decreased expression of the targeted mRNA and it could effect the expression of thousands of protein in a particular cell. An increasing body of evidence suggest that miRNAs action can be modulated by proteins that bind to the same 3'UTRs that are targeted by miRNAs, suggesting that other factors apart from miRNAs and their target sites determine miRNA-modulation of gene expression. We applied an affinity purification protocol using biotinylated let-7 miRNA inhibitor to isolate proteins that are involved in let-7 mediated gene regulation that resulted in an affinity purification of Polypyrimidine Tract Binding protein (PTB). Here we show that PTB interacts with miRNAs and human Argonaute 2 (hAgo2) through RNA as well as identified potential mammalian cellular targets that are co-regulated by PTB and hAgo2. In addition, using genetic approach, we have demonstrated that PTB genetically interacts with Caenorhabditis elegans let-7 indicating a conserved role for PTB in miRNA-mediated gene regulation. |
| doi_str_mv | 10.1371/journal.pone.0033144 |
| format | Article |
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An increasing body of evidence suggest that miRNAs action can be modulated by proteins that bind to the same 3'UTRs that are targeted by miRNAs, suggesting that other factors apart from miRNAs and their target sites determine miRNA-modulation of gene expression. We applied an affinity purification protocol using biotinylated let-7 miRNA inhibitor to isolate proteins that are involved in let-7 mediated gene regulation that resulted in an affinity purification of Polypyrimidine Tract Binding protein (PTB). Here we show that PTB interacts with miRNAs and human Argonaute 2 (hAgo2) through RNA as well as identified potential mammalian cellular targets that are co-regulated by PTB and hAgo2. In addition, using genetic approach, we have demonstrated that PTB genetically interacts with Caenorhabditis elegans let-7 indicating a conserved role for PTB in miRNA-mediated gene regulation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0033144</identifier><identifier>PMID: 22427970</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>3' Untranslated regions ; Affinity ; Animals ; Argonaute 2 protein ; Argonaute Proteins - metabolism ; Base Sequence ; Binding ; Binding sites ; Biology ; Caenorhabditis elegans ; Cancer ; Chromatography, Affinity ; DNA repair ; Enzymes ; Gene expression ; Gene Expression Regulation - genetics ; Gene Knockdown Techniques ; Gene regulation ; Genes ; Genetic regulation ; Genomes ; HeLa Cells ; Humans ; Hypoxia ; Life sciences ; Mammals ; Medical research ; Messenger RNA ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Molecular Sequence Data ; Polypyrimidine Tract-Binding Protein - genetics ; Polypyrimidine Tract-Binding Protein - metabolism ; Protein binding ; Protein purification ; Proteins ; Purification ; Ribonucleic acid ; RNA ; Target recognition</subject><ispartof>PloS one, 2012-03, Vol.7 (3), p.e33144-e33144</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Engels et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Engels et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c625t-af638493ca2fc0bb4f968462602014c57cccde0657a57cc4d10e37037e3f05783</citedby><cites>FETCH-LOGICAL-c625t-af638493ca2fc0bb4f968462602014c57cccde0657a57cc4d10e37037e3f05783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302860/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302860/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22427970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Engels, Bart</creatorcontrib><creatorcontrib>Jannot, Guillaume</creatorcontrib><creatorcontrib>Remenyi, Judit</creatorcontrib><creatorcontrib>Simard, Martin J</creatorcontrib><creatorcontrib>Hutvagner, György</creatorcontrib><title>Polypyrimidine tract binding protein (hnRNP I) is possibly a conserved modulator of miRNA-mediated gene regulation</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>MiRNAs can regulate gene expression through versatile mechanisms that result in increased or decreased expression of the targeted mRNA and it could effect the expression of thousands of protein in a particular cell. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Engels, Bart</au><au>Jannot, Guillaume</au><au>Remenyi, Judit</au><au>Simard, Martin J</au><au>Hutvagner, György</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polypyrimidine tract binding protein (hnRNP I) is possibly a conserved modulator of miRNA-mediated gene regulation</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-03-09</date><risdate>2012</risdate><volume>7</volume><issue>3</issue><spage>e33144</spage><epage>e33144</epage><pages>e33144-e33144</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>MiRNAs can regulate gene expression through versatile mechanisms that result in increased or decreased expression of the targeted mRNA and it could effect the expression of thousands of protein in a particular cell. An increasing body of evidence suggest that miRNAs action can be modulated by proteins that bind to the same 3'UTRs that are targeted by miRNAs, suggesting that other factors apart from miRNAs and their target sites determine miRNA-modulation of gene expression. We applied an affinity purification protocol using biotinylated let-7 miRNA inhibitor to isolate proteins that are involved in let-7 mediated gene regulation that resulted in an affinity purification of Polypyrimidine Tract Binding protein (PTB). Here we show that PTB interacts with miRNAs and human Argonaute 2 (hAgo2) through RNA as well as identified potential mammalian cellular targets that are co-regulated by PTB and hAgo2. In addition, using genetic approach, we have demonstrated that PTB genetically interacts with Caenorhabditis elegans let-7 indicating a conserved role for PTB in miRNA-mediated gene regulation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22427970</pmid><doi>10.1371/journal.pone.0033144</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 3' Untranslated regions Affinity Animals Argonaute 2 protein Argonaute Proteins - metabolism Base Sequence Binding Binding sites Biology Caenorhabditis elegans Cancer Chromatography, Affinity DNA repair Enzymes Gene expression Gene Expression Regulation - genetics Gene Knockdown Techniques Gene regulation Genes Genetic regulation Genomes HeLa Cells Humans Hypoxia Life sciences Mammals Medical research Messenger RNA MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Molecular Sequence Data Polypyrimidine Tract-Binding Protein - genetics Polypyrimidine Tract-Binding Protein - metabolism Protein binding Protein purification Proteins Purification Ribonucleic acid RNA Target recognition |
| title | Polypyrimidine tract binding protein (hnRNP I) is possibly a conserved modulator of miRNA-mediated gene regulation |
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