Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis
Clostridium perfringens is a common inhabitant of the avian and mammalian gastrointestinal tracts and can behave commensally or pathogenically. Some enteric diseases caused by type A C. perfringens, including bovine clostridial abomasitis, remain poorly understood. To investigate the potential basis...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-03, Vol.7 (3), p.e32271-e32271 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e32271 |
|---|---|
| container_issue | 3 |
| container_start_page | e32271 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Nowell, Victoria J Kropinski, Andrew M Songer, J Glenn MacInnes, Janet I Parreira, Valeria R Prescott, John F |
| description | Clostridium perfringens is a common inhabitant of the avian and mammalian gastrointestinal tracts and can behave commensally or pathogenically. Some enteric diseases caused by type A C. perfringens, including bovine clostridial abomasitis, remain poorly understood. To investigate the potential basis of virulence in strains causing this disease, we sequenced the genome of a type A C. perfringens isolate (strain F262) from a case of bovine clostridial abomasitis. The ∼3.34 Mbp chromosome of C. perfringens F262 is predicted to contain 3163 protein-coding genes, 76 tRNA genes, and an integrated plasmid sequence, Cfrag (∼18 kb). In addition, sequences of two complete circular plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), and two incomplete plasmid fragments, pF262A (48.5 kb) and pF262B (50.0 kb), were identified. Comparison of the chromosome sequence of C. perfringens F262 to complete C. perfringens chromosomes, plasmids and phages revealed 261 unique genes. No novel toxin genes related to previously described clostridial toxins were identified: 60% of the 261 unique genes were hypothetical proteins. There was a two base pair deletion in virS, a gene reported to encode the main sensor kinase involved in virulence gene activation. Despite this frameshift mutation, C. perfringens F262 expressed perfringolysin O, alpha-toxin and the beta2-toxin, suggesting that another regulation system might contribute to the pathogenicity of this strain. Two complete plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), unique to this strain of C. perfringens were identified. |
| doi_str_mv | 10.1371/journal.pone.0032271 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1323990549</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477145390</galeid><doaj_id>oai_doaj_org_article_9eafa3d1c66440ebbf635a2f110f0144</doaj_id><sourcerecordid>A477145390</sourcerecordid><originalsourceid>FETCH-LOGICAL-c790t-7c20cc196c91fb72fbee45fec7e9a9ece03d7a6991da42b2f47f2c3e208da4813</originalsourceid><addsrcrecordid>eNqNk9uK2zAQhk1p6W7TvkFpDYUeLpLq4NjRTSGEdhtYWOjpVozlUaLFlrKSvW3evnLjLHFZSjHGZvT9v0YzmiR5TsmM8oK-v3adt1DPds7ijBDOWEEfJOdUcDbNGeEPT_7PkichXBMy54s8f5ycMZZRtsjJefLzAq1rMA1406FVxm5SsFV8od4HE1KnU0jb_Q7TZbqqXWi9qUzXpDv02kcabUhNcDW0mGrvmkgrCNjrSndrLKbqqII6hdI1EExrwtPkkYY64LPhO0m-f_r4bfV5enl1sV4tL6eqEKSdFooRpajIlaC6LJguEbO5RlWgAIEKCa8KyIWgFWSsZDorNFMcGVnEwILySfLy4LuLacihZkFSzrgQZJ6JSKwPROXgWu68acDvpQMj_wSc30jwrVE1SoGggVdU5XmWESxLnfM5ME0p0YRmWfT6MOzWlQ1WCm3roR6Zjles2cqNu5WciSInfbpvBgPvYkNCKxsTFNY1WHRdkIIVMW1OWCTf_pOkhFJGckb7rF79hd5fh4HaQDyqsdrFDFVvKpdZUdBszuPOk2R2DxWfChuj4lXUJsZHgncjQWRa_NVuoAtBrr9--X_26seYfX3CbhHqdhvvYdcaZ8MYzA6g8i4Ej_quHZTIfpKO1ZD9JMlhkqLsxWkr70TH0eG_AV7rGfo</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1323990549</pqid></control><display><type>article</type><title>Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Nowell, Victoria J ; Kropinski, Andrew M ; Songer, J Glenn ; MacInnes, Janet I ; Parreira, Valeria R ; Prescott, John F</creator><contributor>Fitzgerald, J. Ross</contributor><creatorcontrib>Nowell, Victoria J ; Kropinski, Andrew M ; Songer, J Glenn ; MacInnes, Janet I ; Parreira, Valeria R ; Prescott, John F ; Fitzgerald, J. Ross</creatorcontrib><description>Clostridium perfringens is a common inhabitant of the avian and mammalian gastrointestinal tracts and can behave commensally or pathogenically. Some enteric diseases caused by type A C. perfringens, including bovine clostridial abomasitis, remain poorly understood. To investigate the potential basis of virulence in strains causing this disease, we sequenced the genome of a type A C. perfringens isolate (strain F262) from a case of bovine clostridial abomasitis. The ∼3.34 Mbp chromosome of C. perfringens F262 is predicted to contain 3163 protein-coding genes, 76 tRNA genes, and an integrated plasmid sequence, Cfrag (∼18 kb). In addition, sequences of two complete circular plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), and two incomplete plasmid fragments, pF262A (48.5 kb) and pF262B (50.0 kb), were identified. Comparison of the chromosome sequence of C. perfringens F262 to complete C. perfringens chromosomes, plasmids and phages revealed 261 unique genes. No novel toxin genes related to previously described clostridial toxins were identified: 60% of the 261 unique genes were hypothetical proteins. There was a two base pair deletion in virS, a gene reported to encode the main sensor kinase involved in virulence gene activation. Despite this frameshift mutation, C. perfringens F262 expressed perfringolysin O, alpha-toxin and the beta2-toxin, suggesting that another regulation system might contribute to the pathogenicity of this strain. Two complete plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), unique to this strain of C. perfringens were identified.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0032271</identifier><identifier>PMID: 22412860</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biology ; Cattle ; Cattle Diseases - microbiology ; Chromosomes ; Clostridium Infections - microbiology ; Clostridium Infections - veterinary ; Clostridium perfringens ; Clostridium perfringens - genetics ; Clostridium perfringens - isolation & purification ; DNA sequencing ; Frameshift Mutation ; Gastrointestinal system ; Gastrointestinal tract ; Gene deletion ; Gene sequencing ; Genes ; Genome, Bacterial ; Genomes ; Genomics ; Gram-positive bacteria ; Medicine ; Molecular Sequence Annotation ; Mutation ; Pathogenicity ; Pathogens ; Phages ; Plasmids ; Proteins ; Sequence Analysis, DNA ; Stomach Diseases - microbiology ; Stomach Diseases - veterinary ; Toxins ; Transcription activation ; Transfer RNA ; tRNA ; Veterinary Science ; Virulence ; Virulence (Microbiology) ; α-Toxin</subject><ispartof>PloS one, 2012-03, Vol.7 (3), p.e32271-e32271</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Nowell et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Nowell et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c790t-7c20cc196c91fb72fbee45fec7e9a9ece03d7a6991da42b2f47f2c3e208da4813</citedby><cites>FETCH-LOGICAL-c790t-7c20cc196c91fb72fbee45fec7e9a9ece03d7a6991da42b2f47f2c3e208da4813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297601/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3297601/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22412860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Fitzgerald, J. Ross</contributor><creatorcontrib>Nowell, Victoria J</creatorcontrib><creatorcontrib>Kropinski, Andrew M</creatorcontrib><creatorcontrib>Songer, J Glenn</creatorcontrib><creatorcontrib>MacInnes, Janet I</creatorcontrib><creatorcontrib>Parreira, Valeria R</creatorcontrib><creatorcontrib>Prescott, John F</creatorcontrib><title>Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Clostridium perfringens is a common inhabitant of the avian and mammalian gastrointestinal tracts and can behave commensally or pathogenically. Some enteric diseases caused by type A C. perfringens, including bovine clostridial abomasitis, remain poorly understood. To investigate the potential basis of virulence in strains causing this disease, we sequenced the genome of a type A C. perfringens isolate (strain F262) from a case of bovine clostridial abomasitis. The ∼3.34 Mbp chromosome of C. perfringens F262 is predicted to contain 3163 protein-coding genes, 76 tRNA genes, and an integrated plasmid sequence, Cfrag (∼18 kb). In addition, sequences of two complete circular plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), and two incomplete plasmid fragments, pF262A (48.5 kb) and pF262B (50.0 kb), were identified. Comparison of the chromosome sequence of C. perfringens F262 to complete C. perfringens chromosomes, plasmids and phages revealed 261 unique genes. No novel toxin genes related to previously described clostridial toxins were identified: 60% of the 261 unique genes were hypothetical proteins. There was a two base pair deletion in virS, a gene reported to encode the main sensor kinase involved in virulence gene activation. Despite this frameshift mutation, C. perfringens F262 expressed perfringolysin O, alpha-toxin and the beta2-toxin, suggesting that another regulation system might contribute to the pathogenicity of this strain. Two complete plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), unique to this strain of C. perfringens were identified.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biology</subject><subject>Cattle</subject><subject>Cattle Diseases - microbiology</subject><subject>Chromosomes</subject><subject>Clostridium Infections - microbiology</subject><subject>Clostridium Infections - veterinary</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - genetics</subject><subject>Clostridium perfringens - isolation & purification</subject><subject>DNA sequencing</subject><subject>Frameshift Mutation</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>Gene deletion</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Gram-positive bacteria</subject><subject>Medicine</subject><subject>Molecular Sequence Annotation</subject><subject>Mutation</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Phages</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Sequence Analysis, DNA</subject><subject>Stomach Diseases - microbiology</subject><subject>Stomach Diseases - veterinary</subject><subject>Toxins</subject><subject>Transcription activation</subject><subject>Transfer RNA</subject><subject>tRNA</subject><subject>Veterinary Science</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>α-Toxin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9uK2zAQhk1p6W7TvkFpDYUeLpLq4NjRTSGEdhtYWOjpVozlUaLFlrKSvW3evnLjLHFZSjHGZvT9v0YzmiR5TsmM8oK-v3adt1DPds7ijBDOWEEfJOdUcDbNGeEPT_7PkichXBMy54s8f5ycMZZRtsjJefLzAq1rMA1406FVxm5SsFV8od4HE1KnU0jb_Q7TZbqqXWi9qUzXpDv02kcabUhNcDW0mGrvmkgrCNjrSndrLKbqqII6hdI1EExrwtPkkYY64LPhO0m-f_r4bfV5enl1sV4tL6eqEKSdFooRpajIlaC6LJguEbO5RlWgAIEKCa8KyIWgFWSsZDorNFMcGVnEwILySfLy4LuLacihZkFSzrgQZJ6JSKwPROXgWu68acDvpQMj_wSc30jwrVE1SoGggVdU5XmWESxLnfM5ME0p0YRmWfT6MOzWlQ1WCm3roR6Zjles2cqNu5WciSInfbpvBgPvYkNCKxsTFNY1WHRdkIIVMW1OWCTf_pOkhFJGckb7rF79hd5fh4HaQDyqsdrFDFVvKpdZUdBszuPOk2R2DxWfChuj4lXUJsZHgncjQWRa_NVuoAtBrr9--X_26seYfX3CbhHqdhvvYdcaZ8MYzA6g8i4Ej_quHZTIfpKO1ZD9JMlhkqLsxWkr70TH0eG_AV7rGfo</recordid><startdate>20120308</startdate><enddate>20120308</enddate><creator>Nowell, Victoria J</creator><creator>Kropinski, Andrew M</creator><creator>Songer, J Glenn</creator><creator>MacInnes, Janet I</creator><creator>Parreira, Valeria R</creator><creator>Prescott, John F</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120308</creationdate><title>Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis</title><author>Nowell, Victoria J ; Kropinski, Andrew M ; Songer, J Glenn ; MacInnes, Janet I ; Parreira, Valeria R ; Prescott, John F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c790t-7c20cc196c91fb72fbee45fec7e9a9ece03d7a6991da42b2f47f2c3e208da4813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biology</topic><topic>Cattle</topic><topic>Cattle Diseases - microbiology</topic><topic>Chromosomes</topic><topic>Clostridium Infections - microbiology</topic><topic>Clostridium Infections - veterinary</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - genetics</topic><topic>Clostridium perfringens - isolation & purification</topic><topic>DNA sequencing</topic><topic>Frameshift Mutation</topic><topic>Gastrointestinal system</topic><topic>Gastrointestinal tract</topic><topic>Gene deletion</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Gram-positive bacteria</topic><topic>Medicine</topic><topic>Molecular Sequence Annotation</topic><topic>Mutation</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Phages</topic><topic>Plasmids</topic><topic>Proteins</topic><topic>Sequence Analysis, DNA</topic><topic>Stomach Diseases - microbiology</topic><topic>Stomach Diseases - veterinary</topic><topic>Toxins</topic><topic>Transcription activation</topic><topic>Transfer RNA</topic><topic>tRNA</topic><topic>Veterinary Science</topic><topic>Virulence</topic><topic>Virulence (Microbiology)</topic><topic>α-Toxin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nowell, Victoria J</creatorcontrib><creatorcontrib>Kropinski, Andrew M</creatorcontrib><creatorcontrib>Songer, J Glenn</creatorcontrib><creatorcontrib>MacInnes, Janet I</creatorcontrib><creatorcontrib>Parreira, Valeria R</creatorcontrib><creatorcontrib>Prescott, John F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nowell, Victoria J</au><au>Kropinski, Andrew M</au><au>Songer, J Glenn</au><au>MacInnes, Janet I</au><au>Parreira, Valeria R</au><au>Prescott, John F</au><au>Fitzgerald, J. Ross</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-03-08</date><risdate>2012</risdate><volume>7</volume><issue>3</issue><spage>e32271</spage><epage>e32271</epage><pages>e32271-e32271</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Clostridium perfringens is a common inhabitant of the avian and mammalian gastrointestinal tracts and can behave commensally or pathogenically. Some enteric diseases caused by type A C. perfringens, including bovine clostridial abomasitis, remain poorly understood. To investigate the potential basis of virulence in strains causing this disease, we sequenced the genome of a type A C. perfringens isolate (strain F262) from a case of bovine clostridial abomasitis. The ∼3.34 Mbp chromosome of C. perfringens F262 is predicted to contain 3163 protein-coding genes, 76 tRNA genes, and an integrated plasmid sequence, Cfrag (∼18 kb). In addition, sequences of two complete circular plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), and two incomplete plasmid fragments, pF262A (48.5 kb) and pF262B (50.0 kb), were identified. Comparison of the chromosome sequence of C. perfringens F262 to complete C. perfringens chromosomes, plasmids and phages revealed 261 unique genes. No novel toxin genes related to previously described clostridial toxins were identified: 60% of the 261 unique genes were hypothetical proteins. There was a two base pair deletion in virS, a gene reported to encode the main sensor kinase involved in virulence gene activation. Despite this frameshift mutation, C. perfringens F262 expressed perfringolysin O, alpha-toxin and the beta2-toxin, suggesting that another regulation system might contribute to the pathogenicity of this strain. Two complete plasmids, pF262C (4.8 kb) and pF262D (9.1 kb), unique to this strain of C. perfringens were identified.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22412860</pmid><doi>10.1371/journal.pone.0032271</doi><tpages>e32271</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-03, Vol.7 (3), p.e32271-e32271 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1323990549 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Biology Cattle Cattle Diseases - microbiology Chromosomes Clostridium Infections - microbiology Clostridium Infections - veterinary Clostridium perfringens Clostridium perfringens - genetics Clostridium perfringens - isolation & purification DNA sequencing Frameshift Mutation Gastrointestinal system Gastrointestinal tract Gene deletion Gene sequencing Genes Genome, Bacterial Genomes Genomics Gram-positive bacteria Medicine Molecular Sequence Annotation Mutation Pathogenicity Pathogens Phages Plasmids Proteins Sequence Analysis, DNA Stomach Diseases - microbiology Stomach Diseases - veterinary Toxins Transcription activation Transfer RNA tRNA Veterinary Science Virulence Virulence (Microbiology) α-Toxin |
| title | Genome sequencing and analysis of a type A Clostridium perfringens isolate from a case of bovine clostridial abomasitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T11%3A01%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome%20sequencing%20and%20analysis%20of%20a%20type%20A%20Clostridium%20perfringens%20isolate%20from%20a%20case%20of%20bovine%20clostridial%20abomasitis&rft.jtitle=PloS%20one&rft.au=Nowell,%20Victoria%20J&rft.date=2012-03-08&rft.volume=7&rft.issue=3&rft.spage=e32271&rft.epage=e32271&rft.pages=e32271-e32271&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0032271&rft_dat=%3Cgale_plos_%3EA477145390%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1323990549&rft_id=info:pmid/22412860&rft_galeid=A477145390&rft_doaj_id=oai_doaj_org_article_9eafa3d1c66440ebbf635a2f110f0144&rfr_iscdi=true |