De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids
Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome con...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-02, Vol.7 (2), p.e30987-e30987 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e30987 |
|---|---|
| container_issue | 2 |
| container_start_page | e30987 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Li, Haiyan Dong, Yuanyuan Yang, Jing Liu, Xiuming Wang, Yanfang Yao, Na Guan, Lili Wang, Nan Wu, Jinyu Li, Xiaokun |
| description | Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower. |
| doi_str_mv | 10.1371/journal.pone.0030987 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1323854581</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477055620</galeid><doaj_id>oai_doaj_org_article_332de396e5674c78af1ad965efe4f6c0</doaj_id><sourcerecordid>A477055620</sourcerecordid><originalsourceid>FETCH-LOGICAL-c757t-4711d75a5e2c05c7391c7b6fbbc7ec1e44bf41043f0c129524e4e33d584079e43</originalsourceid><addsrcrecordid>eNqNk12P1CAUhhujcdfRf2C0iYnGixmhQGlvTDbr1ySbbOLXLaH0MMOEgRHa0fXXS53uZGr2wnDBCTzvCxzOybKnGC0w4fjNxvfBSbvYeQcLhAiqK34vO8c1KeZlgcj9k_gsexTjBiFGqrJ8mJ0VBSkJK6rz7Pc7yJ3f-7wL0kUVzK7zW8i9zqPU2vqfEHLp2rxbQ25acJ3RRsnOeDcwu75L8R7yFTiIufYh9xZ8NO4oaoyPNy5F0cRBoq3ce-dNGx9nD7S0EZ6M8yz79uH918tP86vrj8vLi6u54ox3c8oxbjmTDAqFmOKkxoo3pW4axUFhoLTRFCNKNFK4qFlBgQIhLaso4jVQMsueH3x31kcxpi0KTApSMcoqnIjlgWi93IhdMFsZboSXRvxd8GElZOiMsiAIKVogdQms5FTxSmos27pkoIHqUqHk9XY8rW-20KqUsiDtxHS648xarPxekKIirB6u-2o0CP5HD7ETWxMVWCsd-D6KuiCMU1TWiXzxD3n340ZqJdP9jdM-HasGT3FBOUeMDRUyyxZ3UGm0sDUqlZg2aX0ieD0RJKaDX91K9jGK5ZfP_89ef5-yL0_YNUjbraO3_VBycQrSA6iCjzGAPuYYIzF0yG02xNAhYuyQJHt2-j9H0W1LkD-CsQzx</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1323854581</pqid></control><display><type>article</type><title>De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Li, Haiyan ; Dong, Yuanyuan ; Yang, Jing ; Liu, Xiuming ; Wang, Yanfang ; Yao, Na ; Guan, Lili ; Wang, Nan ; Wu, Jinyu ; Li, Xiaokun</creator><contributor>Tramontano, Anna</contributor><creatorcontrib>Li, Haiyan ; Dong, Yuanyuan ; Yang, Jing ; Liu, Xiuming ; Wang, Yanfang ; Yao, Na ; Guan, Lili ; Wang, Nan ; Wu, Jinyu ; Li, Xiaokun ; Tramontano, Anna</creatorcontrib><description>Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0030987</identifier><identifier>PMID: 22363528</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Analysis ; Annotations ; Arabidopsis ; Biology ; Biosynthesis ; Biosynthetic Pathways - genetics ; Brassicaceae ; Carthamus tinctorius ; Carthamus tinctorius - genetics ; Cereals ; Cichorium ; Cichorium intybus ; Coffea canephora ; Education ; Engineering research ; Enzymes ; Flavonoids ; Flavonoids - biosynthesis ; Flowers & plants ; Flowers - genetics ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Plant ; Gene Library ; Gene sequencing ; Genes ; Genes, Plant - genetics ; Genetic Association Studies ; Genomes ; Genomics ; Glycine max ; Isoflavones ; Life sciences ; Matthiola incana ; Metabolism ; Molecular Sequence Annotation ; Oleosin ; Pharmaceuticals ; Pisum sativum ; Plant Leaves - genetics ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Seeds ; Seeds - genetics ; Sequence Analysis, DNA ; Solanum tuberosum ; Transcriptome - genetics ; Viral infections</subject><ispartof>PloS one, 2012-02, Vol.7 (2), p.e30987-e30987</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Li et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c757t-4711d75a5e2c05c7391c7b6fbbc7ec1e44bf41043f0c129524e4e33d584079e43</citedby><cites>FETCH-LOGICAL-c757t-4711d75a5e2c05c7391c7b6fbbc7ec1e44bf41043f0c129524e4e33d584079e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283594/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283594/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22363528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tramontano, Anna</contributor><creatorcontrib>Li, Haiyan</creatorcontrib><creatorcontrib>Dong, Yuanyuan</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Liu, Xiuming</creatorcontrib><creatorcontrib>Wang, Yanfang</creatorcontrib><creatorcontrib>Yao, Na</creatorcontrib><creatorcontrib>Guan, Lili</creatorcontrib><creatorcontrib>Wang, Nan</creatorcontrib><creatorcontrib>Wu, Jinyu</creatorcontrib><creatorcontrib>Li, Xiaokun</creatorcontrib><title>De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower.</description><subject>Agriculture</subject><subject>Analysis</subject><subject>Annotations</subject><subject>Arabidopsis</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways - genetics</subject><subject>Brassicaceae</subject><subject>Carthamus tinctorius</subject><subject>Carthamus tinctorius - genetics</subject><subject>Cereals</subject><subject>Cichorium</subject><subject>Cichorium intybus</subject><subject>Coffea canephora</subject><subject>Education</subject><subject>Engineering research</subject><subject>Enzymes</subject><subject>Flavonoids</subject><subject>Flavonoids - biosynthesis</subject><subject>Flowers & plants</subject><subject>Flowers - genetics</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Library</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genes, Plant - genetics</subject><subject>Genetic Association Studies</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Glycine max</subject><subject>Isoflavones</subject><subject>Life sciences</subject><subject>Matthiola incana</subject><subject>Metabolism</subject><subject>Molecular Sequence Annotation</subject><subject>Oleosin</subject><subject>Pharmaceuticals</subject><subject>Pisum sativum</subject><subject>Plant Leaves - genetics</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Reproducibility of Results</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Seeds</subject><subject>Seeds - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Solanum tuberosum</subject><subject>Transcriptome - genetics</subject><subject>Viral infections</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12P1CAUhhujcdfRf2C0iYnGixmhQGlvTDbr1ySbbOLXLaH0MMOEgRHa0fXXS53uZGr2wnDBCTzvCxzOybKnGC0w4fjNxvfBSbvYeQcLhAiqK34vO8c1KeZlgcj9k_gsexTjBiFGqrJ8mJ0VBSkJK6rz7Pc7yJ3f-7wL0kUVzK7zW8i9zqPU2vqfEHLp2rxbQ25acJ3RRsnOeDcwu75L8R7yFTiIufYh9xZ8NO4oaoyPNy5F0cRBoq3ce-dNGx9nD7S0EZ6M8yz79uH918tP86vrj8vLi6u54ox3c8oxbjmTDAqFmOKkxoo3pW4axUFhoLTRFCNKNFK4qFlBgQIhLaso4jVQMsueH3x31kcxpi0KTApSMcoqnIjlgWi93IhdMFsZboSXRvxd8GElZOiMsiAIKVogdQms5FTxSmos27pkoIHqUqHk9XY8rW-20KqUsiDtxHS648xarPxekKIirB6u-2o0CP5HD7ETWxMVWCsd-D6KuiCMU1TWiXzxD3n340ZqJdP9jdM-HasGT3FBOUeMDRUyyxZ3UGm0sDUqlZg2aX0ieD0RJKaDX91K9jGK5ZfP_89ef5-yL0_YNUjbraO3_VBycQrSA6iCjzGAPuYYIzF0yG02xNAhYuyQJHt2-j9H0W1LkD-CsQzx</recordid><startdate>20120221</startdate><enddate>20120221</enddate><creator>Li, Haiyan</creator><creator>Dong, Yuanyuan</creator><creator>Yang, Jing</creator><creator>Liu, Xiuming</creator><creator>Wang, Yanfang</creator><creator>Yao, Na</creator><creator>Guan, Lili</creator><creator>Wang, Nan</creator><creator>Wu, Jinyu</creator><creator>Li, Xiaokun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120221</creationdate><title>De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids</title><author>Li, Haiyan ; Dong, Yuanyuan ; Yang, Jing ; Liu, Xiuming ; Wang, Yanfang ; Yao, Na ; Guan, Lili ; Wang, Nan ; Wu, Jinyu ; Li, Xiaokun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c757t-4711d75a5e2c05c7391c7b6fbbc7ec1e44bf41043f0c129524e4e33d584079e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agriculture</topic><topic>Analysis</topic><topic>Annotations</topic><topic>Arabidopsis</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways - genetics</topic><topic>Brassicaceae</topic><topic>Carthamus tinctorius</topic><topic>Carthamus tinctorius - genetics</topic><topic>Cereals</topic><topic>Cichorium</topic><topic>Cichorium intybus</topic><topic>Coffea canephora</topic><topic>Education</topic><topic>Engineering research</topic><topic>Enzymes</topic><topic>Flavonoids</topic><topic>Flavonoids - biosynthesis</topic><topic>Flowers & plants</topic><topic>Flowers - genetics</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Library</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genes, Plant - genetics</topic><topic>Genetic Association Studies</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Glycine max</topic><topic>Isoflavones</topic><topic>Life sciences</topic><topic>Matthiola incana</topic><topic>Metabolism</topic><topic>Molecular Sequence Annotation</topic><topic>Oleosin</topic><topic>Pharmaceuticals</topic><topic>Pisum sativum</topic><topic>Plant Leaves - genetics</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Reproducibility of Results</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Seeds</topic><topic>Seeds - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Solanum tuberosum</topic><topic>Transcriptome - genetics</topic><topic>Viral infections</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Haiyan</creatorcontrib><creatorcontrib>Dong, Yuanyuan</creatorcontrib><creatorcontrib>Yang, Jing</creatorcontrib><creatorcontrib>Liu, Xiuming</creatorcontrib><creatorcontrib>Wang, Yanfang</creatorcontrib><creatorcontrib>Yao, Na</creatorcontrib><creatorcontrib>Guan, Lili</creatorcontrib><creatorcontrib>Wang, Nan</creatorcontrib><creatorcontrib>Wu, Jinyu</creatorcontrib><creatorcontrib>Li, Xiaokun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Haiyan</au><au>Dong, Yuanyuan</au><au>Yang, Jing</au><au>Liu, Xiuming</au><au>Wang, Yanfang</au><au>Yao, Na</au><au>Guan, Lili</au><au>Wang, Nan</au><au>Wu, Jinyu</au><au>Li, Xiaokun</au><au>Tramontano, Anna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-02-21</date><risdate>2012</risdate><volume>7</volume><issue>2</issue><spage>e30987</spage><epage>e30987</epage><pages>e30987-e30987</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid biosynthesis and metabolism in safflower.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22363528</pmid><doi>10.1371/journal.pone.0030987</doi><tpages>e30987</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-02, Vol.7 (2), p.e30987-e30987 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1323854581 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Agriculture Analysis Annotations Arabidopsis Biology Biosynthesis Biosynthetic Pathways - genetics Brassicaceae Carthamus tinctorius Carthamus tinctorius - genetics Cereals Cichorium Cichorium intybus Coffea canephora Education Engineering research Enzymes Flavonoids Flavonoids - biosynthesis Flowers & plants Flowers - genetics Gene expression Gene Expression Profiling - methods Gene Expression Regulation, Plant Gene Library Gene sequencing Genes Genes, Plant - genetics Genetic Association Studies Genomes Genomics Glycine max Isoflavones Life sciences Matthiola incana Metabolism Molecular Sequence Annotation Oleosin Pharmaceuticals Pisum sativum Plant Leaves - genetics Plant Proteins - genetics Plant Proteins - metabolism Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Seeds Seeds - genetics Sequence Analysis, DNA Solanum tuberosum Transcriptome - genetics Viral infections |
| title | De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T13%3A39%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=De%20novo%20transcriptome%20of%20safflower%20and%20the%20identification%20of%20putative%20genes%20for%20oleosin%20and%20the%20biosynthesis%20of%20flavonoids&rft.jtitle=PloS%20one&rft.au=Li,%20Haiyan&rft.date=2012-02-21&rft.volume=7&rft.issue=2&rft.spage=e30987&rft.epage=e30987&rft.pages=e30987-e30987&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0030987&rft_dat=%3Cgale_plos_%3EA477055620%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1323854581&rft_id=info:pmid/22363528&rft_galeid=A477055620&rft_doaj_id=oai_doaj_org_article_332de396e5674c78af1ad965efe4f6c0&rfr_iscdi=true |