Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry
Black rice (Oryza sativa L. Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is...
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| creator | Yoshimura, Yukihiro Zaima, Nobuhiro Moriyama, Tatsuya Kawamura, Yukio |
| description | Black rice (Oryza sativa L. Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is limited because methodologies for investigating the localization such as determining specific antibodies to anthocyanin, have not yet been developed Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a suitable tool for investigating the localization of metabolites. In this study, we identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides in crude extracts from black rice by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS. |
| doi_str_mv | 10.1371/journal.pone.0031285 |
| format | Article |
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Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is limited because methodologies for investigating the localization such as determining specific antibodies to anthocyanin, have not yet been developed Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a suitable tool for investigating the localization of metabolites. In this study, we identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides in crude extracts from black rice by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0031285</identifier><identifier>PMID: 22363605</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actinidia chinensis ; Adhesives ; Agriculture ; Animal behavior ; Anthocyanins ; Anthocyanins - chemistry ; Anthocyanins - metabolism ; Antibodies ; Arabidopsis ; Arabidopsis thaliana ; Biological Transport ; Biology ; Biosynthesis ; Chemistry ; Cultivars ; Desorption ; Flowers - metabolism ; Genes ; Health promotion ; Hexose ; Identification ; Imaging, Three-Dimensional - methods ; Ionization ; Ions ; Lasers ; Litchi chinensis ; Localization ; Mass spectrometry ; Mass spectroscopy ; Metabolites ; Monosaccharides ; Oryza - metabolism ; Oryza sativa japonica ; Pentose ; Pericarp ; Pigmentation - physiology ; Plant Extracts - chemistry ; Rice ; Scientific imaging ; Seeds ; Species ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Spectroscopy ; Studies ; Sugar ; Transcription factors</subject><ispartof>PloS one, 2012-02, Vol.7 (2), p.e31285-e31285</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Yoshimura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Yoshimura et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c757t-dc672f7f385641928e7e0fd98b30ad88a5d433c74ffcee1b05cfc1402093099f3</citedby><cites>FETCH-LOGICAL-c757t-dc672f7f385641928e7e0fd98b30ad88a5d433c74ffcee1b05cfc1402093099f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281930/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3281930/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22363605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Bonaventure, Gustavo</contributor><creatorcontrib>Yoshimura, Yukihiro</creatorcontrib><creatorcontrib>Zaima, Nobuhiro</creatorcontrib><creatorcontrib>Moriyama, Tatsuya</creatorcontrib><creatorcontrib>Kawamura, Yukio</creatorcontrib><title>Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Black rice (Oryza sativa L. 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We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS.</description><subject>Actinidia chinensis</subject><subject>Adhesives</subject><subject>Agriculture</subject><subject>Animal behavior</subject><subject>Anthocyanins</subject><subject>Anthocyanins - chemistry</subject><subject>Anthocyanins - metabolism</subject><subject>Antibodies</subject><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>Biological Transport</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Chemistry</subject><subject>Cultivars</subject><subject>Desorption</subject><subject>Flowers - metabolism</subject><subject>Genes</subject><subject>Health promotion</subject><subject>Hexose</subject><subject>Identification</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Ionization</subject><subject>Ions</subject><subject>Lasers</subject><subject>Litchi chinensis</subject><subject>Localization</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Monosaccharides</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa japonica</subject><subject>Pentose</subject><subject>Pericarp</subject><subject>Pigmentation - physiology</subject><subject>Plant Extracts - chemistry</subject><subject>Rice</subject><subject>Scientific imaging</subject><subject>Seeds</subject><subject>Species</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Spectroscopy</subject><subject>Studies</subject><subject>Sugar</subject><subject>Transcription factors</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk8tu1DAUhiMEoqXwBggiIYFYzGDHcZxskKpyG6lSJW5by3GOMy5OHGynYvr0OJ20mqAuUBa-5Pt_2-eSJM8xWmPC8LtLO7pemPVge1gjRHBW0gfJMa5ItioyRB4ezI-SJ95fIkRJWRSPk6MsIwUpED1Orj9opcBBH1JjpTD6WgRt-3QQIYDrfWpVKvqwtXInet2nfgCpwadxGraQDuC0FG6YsNoI-SuNa0gdXIEw0KT1LtWdaHXfpp3w_kYenO0guN3T5JESxsOzeTxJfnz6-P3sy-r84vPm7PR8JRllYdXIgmWKKVLSIsdVVgIDpJqqrAkSTVkK2uSESJYrJQFwjahUEucoQxVBVaXISfJy7zsY6_kcNs8xyQhlGJcsEps90VhxyQcXr-x23ArNbzasa7lwQUsDnFYYVwBlViORI9rUEuVYEKIUaoCpLHq9n08b6w4aGSPrhFmYLv_0estbe8VJVsZ8oWjwZjZw9vcIPvBOewnGiB7s6Hk1XbsoGY3kq3_I-x83U23MCNe9svFYOXny05wxxChlJFLre6j4NdBpGUtM6bi_ELxdCCIT4E9oxeg933z7-v_sxc8l-_qA3cYyCltvzThVpV-C-R6UznrvQN3FGCM-dchtNPjUIXzukCh7cZifO9FtS5C_eAINYw</recordid><startdate>20120217</startdate><enddate>20120217</enddate><creator>Yoshimura, Yukihiro</creator><creator>Zaima, Nobuhiro</creator><creator>Moriyama, Tatsuya</creator><creator>Kawamura, Yukio</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120217</creationdate><title>Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry</title><author>Yoshimura, Yukihiro ; Zaima, Nobuhiro ; Moriyama, Tatsuya ; Kawamura, Yukio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c757t-dc672f7f385641928e7e0fd98b30ad88a5d433c74ffcee1b05cfc1402093099f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Actinidia chinensis</topic><topic>Adhesives</topic><topic>Agriculture</topic><topic>Animal behavior</topic><topic>Anthocyanins</topic><topic>Anthocyanins - chemistry</topic><topic>Anthocyanins - metabolism</topic><topic>Antibodies</topic><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>Biological Transport</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Chemistry</topic><topic>Cultivars</topic><topic>Desorption</topic><topic>Flowers - metabolism</topic><topic>Genes</topic><topic>Health promotion</topic><topic>Hexose</topic><topic>Identification</topic><topic>Imaging, Three-Dimensional - methods</topic><topic>Ionization</topic><topic>Ions</topic><topic>Lasers</topic><topic>Litchi chinensis</topic><topic>Localization</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolites</topic><topic>Monosaccharides</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa japonica</topic><topic>Pentose</topic><topic>Pericarp</topic><topic>Pigmentation - physiology</topic><topic>Plant Extracts - chemistry</topic><topic>Rice</topic><topic>Scientific imaging</topic><topic>Seeds</topic><topic>Species</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshimura, Yukihiro</au><au>Zaima, Nobuhiro</au><au>Moriyama, Tatsuya</au><au>Kawamura, Yukio</au><au>Bonaventure, Gustavo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-02-17</date><risdate>2012</risdate><volume>7</volume><issue>2</issue><spage>e31285</spage><epage>e31285</epage><pages>e31285-e31285</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Black rice (Oryza sativa L. Japonica) contains high levels of anthocyanins in the pericarp and is considered an effective health-promoting food. Several studies have identified the molecular species of anthocyanins in black rice, but information about the localization of each anthocyanin species is limited because methodologies for investigating the localization such as determining specific antibodies to anthocyanin, have not yet been developed Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a suitable tool for investigating the localization of metabolites. In this study, we identified 7 species of anthocyanin monoglycosides and 2 species of anthocyanin diglycosides in crude extracts from black rice by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis. We also analyzed black rice sections by MALDI-IMS and found 2 additional species of anthocyanin pentosides and revealed different localization patterns of anthocyanin species composed of different sugar moieties. Anthocyanin species composed of a pentose moiety (cyanidin-3-O-pentoside and petunidin-3-O-pentoside) were localized in the entire pericarp, whereas anthocyanin species composed of a hexose moiety (cyanidin-3-O-hexoside and peonidin-3-O-hexoside) were focally localized in the dorsal pericarp. These results indicate that anthocyanin species composed of different sugar moieties exhibit different localization patterns in the pericarp of black rice. This is the first detailed investigation into the localization of molecular species of anthocyanins by MALDI-IMS.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22363605</pmid><doi>10.1371/journal.pone.0031285</doi><tpages>e31285</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actinidia chinensis Adhesives Agriculture Animal behavior Anthocyanins Anthocyanins - chemistry Anthocyanins - metabolism Antibodies Arabidopsis Arabidopsis thaliana Biological Transport Biology Biosynthesis Chemistry Cultivars Desorption Flowers - metabolism Genes Health promotion Hexose Identification Imaging, Three-Dimensional - methods Ionization Ions Lasers Litchi chinensis Localization Mass spectrometry Mass spectroscopy Metabolites Monosaccharides Oryza - metabolism Oryza sativa japonica Pentose Pericarp Pigmentation - physiology Plant Extracts - chemistry Rice Scientific imaging Seeds Species Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Spectroscopy Studies Sugar Transcription factors |
| title | Different localization patterns of anthocyanin species in the pericarp of black rice revealed by imaging mass spectrometry |
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