RNA interference of gonadotropin-inhibitory hormone gene induces arousal in songbirds

Gonadotropin-inhibitory hormone (GnIH) was originally identified in quail as a hypothalamic neuropeptide inhibitor of pituitary gonadotropin synthesis and release. However, GnIH neuronal fibers do not only terminate in the median eminence to control anterior pituitary function but also extend widely...

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Veröffentlicht in:PloS one 2012-01, Vol.7 (1), p.e30202-e30202
Hauptverfasser: Ubuka, Takayoshi, Mukai, Motoko, Wolfe, Jordan, Beverly, Ryan, Clegg, Sarah, Wang, Ariel, Hsia, Serena, Li, Molly, Krause, Jesse S, Mizuno, Takanobu, Fukuda, Yujiro, Tsutsui, Kazuyoshi, Bentley, George E, Wingfield, John C
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container_title PloS one
container_volume 7
creator Ubuka, Takayoshi
Mukai, Motoko
Wolfe, Jordan
Beverly, Ryan
Clegg, Sarah
Wang, Ariel
Hsia, Serena
Li, Molly
Krause, Jesse S
Mizuno, Takanobu
Fukuda, Yujiro
Tsutsui, Kazuyoshi
Bentley, George E
Wingfield, John C
description Gonadotropin-inhibitory hormone (GnIH) was originally identified in quail as a hypothalamic neuropeptide inhibitor of pituitary gonadotropin synthesis and release. However, GnIH neuronal fibers do not only terminate in the median eminence to control anterior pituitary function but also extend widely in the brain, suggesting it has multiple roles in the regulation of behavior. To identify the role of GnIH neurons in the regulation of behavior, we investigated the effect of RNA interference (RNAi) of the GnIH gene on the behavior of white-crowned sparrows, a highly social songbird species. Administration of small interfering RNA against GnIH precursor mRNA into the third ventricle of male and female birds reduced resting time, spontaneous production of complex vocalizations, and stimulated brief agonistic vocalizations. GnIH RNAi further enhanced song production of short duration in male birds when they were challenged by playbacks of novel male songs. These behaviors resembled those of breeding birds during territorial defense. The overall results suggest that GnIH gene silencing induces arousal. In addition, the activities of male and female birds were negatively correlated with GnIH mRNA expression in the paraventricular nucleus. Density of GnIH neuronal fibers in the ventral tegmental area was decreased by GnIH RNAi treatment in female birds, and the number of gonadotropin-releasing hormone neurons that received close appositions of GnIH neuronal fiber terminals was negatively correlated with the activity of male birds. In summary, GnIH may decrease arousal level resulting in the inhibition of specific motivated behavior such as in reproductive contexts.
doi_str_mv 10.1371/journal.pone.0030202
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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ubuka, Takayoshi</au><au>Mukai, Motoko</au><au>Wolfe, Jordan</au><au>Beverly, Ryan</au><au>Clegg, Sarah</au><au>Wang, Ariel</au><au>Hsia, Serena</au><au>Li, Molly</au><au>Krause, Jesse S</au><au>Mizuno, Takanobu</au><au>Fukuda, Yujiro</au><au>Tsutsui, Kazuyoshi</au><au>Bentley, George E</au><au>Wingfield, John C</au><au>Ebihara, Shizufumi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA interference of gonadotropin-inhibitory hormone gene induces arousal in songbirds</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-01-18</date><risdate>2012</risdate><volume>7</volume><issue>1</issue><spage>e30202</spage><epage>e30202</epage><pages>e30202-e30202</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Gonadotropin-inhibitory hormone (GnIH) was originally identified in quail as a hypothalamic neuropeptide inhibitor of pituitary gonadotropin synthesis and release. However, GnIH neuronal fibers do not only terminate in the median eminence to control anterior pituitary function but also extend widely in the brain, suggesting it has multiple roles in the regulation of behavior. To identify the role of GnIH neurons in the regulation of behavior, we investigated the effect of RNA interference (RNAi) of the GnIH gene on the behavior of white-crowned sparrows, a highly social songbird species. Administration of small interfering RNA against GnIH precursor mRNA into the third ventricle of male and female birds reduced resting time, spontaneous production of complex vocalizations, and stimulated brief agonistic vocalizations. GnIH RNAi further enhanced song production of short duration in male birds when they were challenged by playbacks of novel male songs. These behaviors resembled those of breeding birds during territorial defense. The overall results suggest that GnIH gene silencing induces arousal. In addition, the activities of male and female birds were negatively correlated with GnIH mRNA expression in the paraventricular nucleus. Density of GnIH neuronal fibers in the ventral tegmental area was decreased by GnIH RNAi treatment in female birds, and the number of gonadotropin-releasing hormone neurons that received close appositions of GnIH neuronal fiber terminals was negatively correlated with the activity of male birds. In summary, GnIH may decrease arousal level resulting in the inhibition of specific motivated behavior such as in reproductive contexts.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22279571</pmid><doi>10.1371/journal.pone.0030202</doi><tpages>e30202</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
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issn 1932-6203
1932-6203
language eng
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source MEDLINE; Directory of Open Access Journals; PubMed (Medline); Public Library of Science; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library
subjects Analysis of Variance
Animal breeding
Animal reproduction
Animals
Arousal
Arousal - genetics
Arousal - physiology
Avian Proteins - genetics
Avian Proteins - metabolism
Biology
Birds
Brain
Brain - cytology
Brain - metabolism
Breeding
Female
Females
Fibers
Gene Expression
Gene silencing
Genes
Genetic engineering
Gonadotropin-releasing hormone
Gonadotropins
Hypothalamic Hormones - genetics
Hypothalamic Hormones - metabolism
Hypothalamus
Immunohistochemistry
In Situ Hybridization
Interference
Male
Males
Medicine
Motivation
Motor Activity - physiology
Mutation
Neurobiology
Neurological disorders
Neurons
Neurons - metabolism
Neuropeptides
Neurosciences
Paraventricular nucleus
Peptides
Physiology
Pituitary (anterior)
Pituitary hormones
Playbacks
Proteins
Reproductive behavior
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA Interference
RNA Precursors - genetics
RNA Precursors - metabolism
RNA-mediated interference
Rodents
Sexual behavior
siRNA
Sleep disorders
Social and Behavioral Sciences
Song
Songbirds
Testosterone - blood
Ventral tegmentum
Ventricle
Ventricles (cerebral)
Vocalization behavior
Vocalization, Animal - physiology
title RNA interference of gonadotropin-inhibitory hormone gene induces arousal in songbirds
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