The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe
Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transc...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-01, Vol.7 (1), p.e30067 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | e30067 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Matia-Gonzalez, Ana M Sotelo, Jael Rodriguez-Gabriel, Miguel A |
| description | Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transcription of many genes required for the initial steps of conjugation and meiosis, and its deficiency leads to sterility. Ste11 activity is mainly regulated at two levels: phosphorylation and abundance of its mRNA. Csx1 is an RNA binding protein that we have previously described to bind and regulate the turnover rate of the mRNA encoding the transcription factor Atf1 in the presence of oxidative stress. We have observed that Csx1-deficient cells have defects in sexual differentiation and are partially sterile. We investigated how Csx1 is regulating this process in S. pombe. Csx1 associates with ste11+ mRNA and cells lacking Csx1 are sterile with a reduced amount of ste11+ mRNA. Overexpression of ste11+ mRNA completely rescues the mating deficiencies of csx1Δ cells. Here, we present a novel mechanism of ste11+ mRNA positive regulation through the activity of Csx1, an RNA binding protein that also have key functions in the response to oxidative stress in fission yeast. This finding opens interesting question about the possible coordination of sexual differentiation and oxidative stress response in eukaryotes and the role of RNA binding proteins in the adaptation to environmental signals. |
| doi_str_mv | 10.1371/journal.pone.0030067 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1322478435</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477167484</galeid><doaj_id>oai_doaj_org_article_72ad1c3219a24fc28504b0348b55d364</doaj_id><sourcerecordid>A477167484</sourcerecordid><originalsourceid>FETCH-LOGICAL-c723t-5ad03163d64b22e2d3c04c0af2055d1a1d2c4959e10b74348936ca4490dba51e3</originalsourceid><addsrcrecordid>eNqNk1tr1EAUxwdRbLv6DUQDguLDrnPLJHkRlqXqQrHQVsGn4WRmspklyWwziWz99E7ctGykD5KHueR3_ufMuSD0iuAFYQn5uHV920C12LnGLDBmGIvkCTolGaNzQTF7erQ_QWfebzGOWSrEc3RCKQ3blJ6inzelia6-LaPcNto2m2jXus7YJlr5PRkOdTj6yJt9D1WkbVGY1jSdhc66JgrctSrtb-dBqRICfacCvXN1bl6gZwVU3rwc1xn6_vn8ZvV1fnH5Zb1aXsxVQlk3j0FjRgTTgueUGqqZwlxhKCiOY02AaKp4FmeG4DzhjKcZEwo4z7DOISaGzdCbg-6ucl6OWfGSMEp5knIWB2J9ILSDrdy1tob2Tjqw8u-FazcS2s6qysiEgiaKUZIB5YWiaYx5joPXPATDBA9an0ZvfV4brUIuWqgmotM_jS3lxv2SjMaChnfO0PtRoHW3vfGdrK1XpqqgMa73MqMiDUUKpZuht_-Qjz9upDYQ4rdN4YJbNWjKJU8SIhKeDmEvHqHCp01tVeigwob7icGHiUFgOrPvNtB7L9fXV__PXv6Ysu-O2NJA1ZXeVf3QTn4K8gOoWud9a4qHHBMshwG4z4YcBkCOAxDMXh_X58HovuPZH-gp_zU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1322478435</pqid></control><display><type>article</type><title>The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Matia-Gonzalez, Ana M ; Sotelo, Jael ; Rodriguez-Gabriel, Miguel A</creator><contributor>Mata, Juan</contributor><creatorcontrib>Matia-Gonzalez, Ana M ; Sotelo, Jael ; Rodriguez-Gabriel, Miguel A ; Mata, Juan</creatorcontrib><description>Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transcription of many genes required for the initial steps of conjugation and meiosis, and its deficiency leads to sterility. Ste11 activity is mainly regulated at two levels: phosphorylation and abundance of its mRNA. Csx1 is an RNA binding protein that we have previously described to bind and regulate the turnover rate of the mRNA encoding the transcription factor Atf1 in the presence of oxidative stress. We have observed that Csx1-deficient cells have defects in sexual differentiation and are partially sterile. We investigated how Csx1 is regulating this process in S. pombe. Csx1 associates with ste11+ mRNA and cells lacking Csx1 are sterile with a reduced amount of ste11+ mRNA. Overexpression of ste11+ mRNA completely rescues the mating deficiencies of csx1Δ cells. Here, we present a novel mechanism of ste11+ mRNA positive regulation through the activity of Csx1, an RNA binding protein that also have key functions in the response to oxidative stress in fission yeast. This finding opens interesting question about the possible coordination of sexual differentiation and oxidative stress response in eukaryotes and the role of RNA binding proteins in the adaptation to environmental signals.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0030067</identifier><identifier>PMID: 22253882</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology ; Cell cycle ; Cell growth ; Cloning ; Conjugation ; Differentiation ; Efficiency ; Eukaryotes ; Fission ; G1 Phase Cell Cycle Checkpoints - drug effects ; Gene expression ; Gene Expression Regulation, Fungal - drug effects ; Genetic aspects ; Genomes ; Kinases ; Meiosis ; Messenger RNA ; mRNA turnover ; Nitrogen ; Nitrogen - deficiency ; Nitrogen - pharmacology ; Oxidative stress ; Phenotype ; Pheromones ; Phosphorylation ; Protein binding ; Protein Binding - drug effects ; Proteins ; Regulatory proteins ; Reproduction - drug effects ; Ribonucleic acid ; RNA ; RNA, Fungal - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA-binding protein ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; Schizosaccharomyces - cytology ; Schizosaccharomyces - drug effects ; Schizosaccharomyces - genetics ; Schizosaccharomyces - metabolism ; Schizosaccharomyces pombe ; Schizosaccharomyces pombe Proteins - genetics ; Schizosaccharomyces pombe Proteins - metabolism ; Sex differentiation ; Sterility ; Transcription (Genetics) ; Transcription factors ; Transcription Factors - metabolism ; Transcription, Genetic - drug effects ; Turnover rate ; Yeast</subject><ispartof>PloS one, 2012-01, Vol.7 (1), p.e30067</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Matia-Gonzalez et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Matia-Gonzalez et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c723t-5ad03163d64b22e2d3c04c0af2055d1a1d2c4959e10b74348936ca4490dba51e3</citedby><cites>FETCH-LOGICAL-c723t-5ad03163d64b22e2d3c04c0af2055d1a1d2c4959e10b74348936ca4490dba51e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256216/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256216/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23847,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22253882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mata, Juan</contributor><creatorcontrib>Matia-Gonzalez, Ana M</creatorcontrib><creatorcontrib>Sotelo, Jael</creatorcontrib><creatorcontrib>Rodriguez-Gabriel, Miguel A</creatorcontrib><title>The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transcription of many genes required for the initial steps of conjugation and meiosis, and its deficiency leads to sterility. Ste11 activity is mainly regulated at two levels: phosphorylation and abundance of its mRNA. Csx1 is an RNA binding protein that we have previously described to bind and regulate the turnover rate of the mRNA encoding the transcription factor Atf1 in the presence of oxidative stress. We have observed that Csx1-deficient cells have defects in sexual differentiation and are partially sterile. We investigated how Csx1 is regulating this process in S. pombe. Csx1 associates with ste11+ mRNA and cells lacking Csx1 are sterile with a reduced amount of ste11+ mRNA. Overexpression of ste11+ mRNA completely rescues the mating deficiencies of csx1Δ cells. Here, we present a novel mechanism of ste11+ mRNA positive regulation through the activity of Csx1, an RNA binding protein that also have key functions in the response to oxidative stress in fission yeast. This finding opens interesting question about the possible coordination of sexual differentiation and oxidative stress response in eukaryotes and the role of RNA binding proteins in the adaptation to environmental signals.</description><subject>Biology</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cloning</subject><subject>Conjugation</subject><subject>Differentiation</subject><subject>Efficiency</subject><subject>Eukaryotes</subject><subject>Fission</subject><subject>G1 Phase Cell Cycle Checkpoints - drug effects</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Fungal - drug effects</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Kinases</subject><subject>Meiosis</subject><subject>Messenger RNA</subject><subject>mRNA turnover</subject><subject>Nitrogen</subject><subject>Nitrogen - deficiency</subject><subject>Nitrogen - pharmacology</subject><subject>Oxidative stress</subject><subject>Phenotype</subject><subject>Pheromones</subject><subject>Phosphorylation</subject><subject>Protein binding</subject><subject>Protein Binding - drug effects</subject><subject>Proteins</subject><subject>Regulatory proteins</subject><subject>Reproduction - drug effects</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Fungal - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-binding protein</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Schizosaccharomyces - cytology</subject><subject>Schizosaccharomyces - drug effects</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces - metabolism</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins - genetics</subject><subject>Schizosaccharomyces pombe Proteins - metabolism</subject><subject>Sex differentiation</subject><subject>Sterility</subject><subject>Transcription (Genetics)</subject><subject>Transcription factors</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic - drug effects</subject><subject>Turnover rate</subject><subject>Yeast</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1tr1EAUxwdRbLv6DUQDguLDrnPLJHkRlqXqQrHQVsGn4WRmspklyWwziWz99E7ctGykD5KHueR3_ufMuSD0iuAFYQn5uHV920C12LnGLDBmGIvkCTolGaNzQTF7erQ_QWfebzGOWSrEc3RCKQ3blJ6inzelia6-LaPcNto2m2jXus7YJlr5PRkOdTj6yJt9D1WkbVGY1jSdhc66JgrctSrtb-dBqRICfacCvXN1bl6gZwVU3rwc1xn6_vn8ZvV1fnH5Zb1aXsxVQlk3j0FjRgTTgueUGqqZwlxhKCiOY02AaKp4FmeG4DzhjKcZEwo4z7DOISaGzdCbg-6ucl6OWfGSMEp5knIWB2J9ILSDrdy1tob2Tjqw8u-FazcS2s6qysiEgiaKUZIB5YWiaYx5joPXPATDBA9an0ZvfV4brUIuWqgmotM_jS3lxv2SjMaChnfO0PtRoHW3vfGdrK1XpqqgMa73MqMiDUUKpZuht_-Qjz9upDYQ4rdN4YJbNWjKJU8SIhKeDmEvHqHCp01tVeigwob7icGHiUFgOrPvNtB7L9fXV__PXv6Ysu-O2NJA1ZXeVf3QTn4K8gOoWud9a4qHHBMshwG4z4YcBkCOAxDMXh_X58HovuPZH-gp_zU</recordid><startdate>20120111</startdate><enddate>20120111</enddate><creator>Matia-Gonzalez, Ana M</creator><creator>Sotelo, Jael</creator><creator>Rodriguez-Gabriel, Miguel A</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120111</creationdate><title>The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe</title><author>Matia-Gonzalez, Ana M ; Sotelo, Jael ; Rodriguez-Gabriel, Miguel A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c723t-5ad03163d64b22e2d3c04c0af2055d1a1d2c4959e10b74348936ca4490dba51e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biology</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cloning</topic><topic>Conjugation</topic><topic>Differentiation</topic><topic>Efficiency</topic><topic>Eukaryotes</topic><topic>Fission</topic><topic>G1 Phase Cell Cycle Checkpoints - drug effects</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Fungal - drug effects</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Kinases</topic><topic>Meiosis</topic><topic>Messenger RNA</topic><topic>mRNA turnover</topic><topic>Nitrogen</topic><topic>Nitrogen - deficiency</topic><topic>Nitrogen - pharmacology</topic><topic>Oxidative stress</topic><topic>Phenotype</topic><topic>Pheromones</topic><topic>Phosphorylation</topic><topic>Protein binding</topic><topic>Protein Binding - drug effects</topic><topic>Proteins</topic><topic>Regulatory proteins</topic><topic>Reproduction - drug effects</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Fungal - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-binding protein</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Schizosaccharomyces - cytology</topic><topic>Schizosaccharomyces - drug effects</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces - metabolism</topic><topic>Schizosaccharomyces pombe</topic><topic>Schizosaccharomyces pombe Proteins - genetics</topic><topic>Schizosaccharomyces pombe Proteins - metabolism</topic><topic>Sex differentiation</topic><topic>Sterility</topic><topic>Transcription (Genetics)</topic><topic>Transcription factors</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic - drug effects</topic><topic>Turnover rate</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matia-Gonzalez, Ana M</creatorcontrib><creatorcontrib>Sotelo, Jael</creatorcontrib><creatorcontrib>Rodriguez-Gabriel, Miguel A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matia-Gonzalez, Ana M</au><au>Sotelo, Jael</au><au>Rodriguez-Gabriel, Miguel A</au><au>Mata, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-01-11</date><risdate>2012</risdate><volume>7</volume><issue>1</issue><spage>e30067</spage><pages>e30067-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Sexual differentiation is a highly regulated process in the fission yeast Schizosaccharomyces pombe and is triggered by nutrient depletion, mainly nitrogen source. One of the key regulatory proteins in fission yeast sexual differentiation is the transcription factor Ste11. Ste11 regulates the transcription of many genes required for the initial steps of conjugation and meiosis, and its deficiency leads to sterility. Ste11 activity is mainly regulated at two levels: phosphorylation and abundance of its mRNA. Csx1 is an RNA binding protein that we have previously described to bind and regulate the turnover rate of the mRNA encoding the transcription factor Atf1 in the presence of oxidative stress. We have observed that Csx1-deficient cells have defects in sexual differentiation and are partially sterile. We investigated how Csx1 is regulating this process in S. pombe. Csx1 associates with ste11+ mRNA and cells lacking Csx1 are sterile with a reduced amount of ste11+ mRNA. Overexpression of ste11+ mRNA completely rescues the mating deficiencies of csx1Δ cells. Here, we present a novel mechanism of ste11+ mRNA positive regulation through the activity of Csx1, an RNA binding protein that also have key functions in the response to oxidative stress in fission yeast. This finding opens interesting question about the possible coordination of sexual differentiation and oxidative stress response in eukaryotes and the role of RNA binding proteins in the adaptation to environmental signals.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22253882</pmid><doi>10.1371/journal.pone.0030067</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-01, Vol.7 (1), p.e30067 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1322478435 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Biology Cell cycle Cell growth Cloning Conjugation Differentiation Efficiency Eukaryotes Fission G1 Phase Cell Cycle Checkpoints - drug effects Gene expression Gene Expression Regulation, Fungal - drug effects Genetic aspects Genomes Kinases Meiosis Messenger RNA mRNA turnover Nitrogen Nitrogen - deficiency Nitrogen - pharmacology Oxidative stress Phenotype Pheromones Phosphorylation Protein binding Protein Binding - drug effects Proteins Regulatory proteins Reproduction - drug effects Ribonucleic acid RNA RNA, Fungal - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism RNA-binding protein RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Schizosaccharomyces - cytology Schizosaccharomyces - drug effects Schizosaccharomyces - genetics Schizosaccharomyces - metabolism Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins - genetics Schizosaccharomyces pombe Proteins - metabolism Sex differentiation Sterility Transcription (Genetics) Transcription factors Transcription Factors - metabolism Transcription, Genetic - drug effects Turnover rate Yeast |
| title | The RNA binding protein Csx1 promotes sexual differentiation in Schizosaccharomyces pombe |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T17%3A43%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20RNA%20binding%20protein%20Csx1%20promotes%20sexual%20differentiation%20in%20Schizosaccharomyces%20pombe&rft.jtitle=PloS%20one&rft.au=Matia-Gonzalez,%20Ana%20M&rft.date=2012-01-11&rft.volume=7&rft.issue=1&rft.spage=e30067&rft.pages=e30067-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0030067&rft_dat=%3Cgale_plos_%3EA477167484%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1322478435&rft_id=info:pmid/22253882&rft_galeid=A477167484&rft_doaj_id=oai_doaj_org_article_72ad1c3219a24fc28504b0348b55d364&rfr_iscdi=true |