The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi

G protein-coupled receptors (GPCRs) in humans are classified into the five main families named Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin according to the GRAFS classification. Previous results show that these mammalian GRAFS families are well represented in the Metazoan lineages, but the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:PloS one 2012-01, Vol.7 (1), p.e29817-e29817
Hauptverfasser: Krishnan, Arunkumar, Almén, Markus Sällman, Fredriksson, Robert, Schiöth, Helgi B
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page e29817
container_issue 1
container_start_page e29817
container_title PloS one
container_volume 7
creator Krishnan, Arunkumar
Almén, Markus Sällman
Fredriksson, Robert
Schiöth, Helgi B
description G protein-coupled receptors (GPCRs) in humans are classified into the five main families named Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin according to the GRAFS classification. Previous results show that these mammalian GRAFS families are well represented in the Metazoan lineages, but they have not been shown to be present in Fungi. Here, we systematically mined 79 fungal genomes and provide the first evidence that four of the five main mammalian families of GPCRs, namely Rhodopsin, Adhesion, Glutamate and Frizzled, are present in Fungi and found 142 novel sequences between them. Significantly, we provide strong evidence that the Rhodopsin family emerged from the cAMP receptor family in an event close to the split of Opisthokonts and not in Placozoa, as earlier assumed. The Rhodopsin family then expanded greatly in Metazoans while the cAMP receptor family is found in 3 invertebrate species and lost in the vertebrates. We estimate that the Adhesion and Frizzled families evolved before the split of Unikonts from a common ancestor of all major eukaryotic lineages. Also, the study highlights that the fungal Adhesion receptors do not have N-terminal domains whereas the fungal Glutamate receptors have a broad repertoire of mammalian-like N-terminal domains. Further, mining of the close unicellular relatives of the Metazoan lineage, Salpingoeca rosetta and Capsaspora owczarzaki, obtained a rich group of both the Adhesion and Glutamate families, which in particular provided insight to the early emergence of the N-terminal domains of the Adhesion family. We identified 619 Fungi specific GPCRs across 79 genomes and revealed that Blastocladiomycota and Chytridiomycota phylum have Metazoan-like GPCRs rather than the GPCRs specific for Fungi. Overall, this study provides the first evidence of the presence of four of the five main GRAFS families in Fungi and clarifies the early evolutionary history of the GPCR superfamily.
doi_str_mv 10.1371/journal.pone.0029817
format Article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1322080071</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477171698</galeid><doaj_id>oai_doaj_org_article_b429fdfd6f514a27b6c8519bd97b88d4</doaj_id><sourcerecordid>A477171698</sourcerecordid><originalsourceid>FETCH-LOGICAL-c794t-3be81db393123c7ee0405a5f598af75cb22c69046ff8cdd8078298dc7bedd5703</originalsourceid><addsrcrecordid>eNqNk1tv0zAYhiMEYmPwDxBEQgIhtcV2Eh-4QKoKK5UmDZWxW8vxIXFJ4hInHPbrcdtsatAukC9i2c_72nn9fVH0HIIZTAh8t3F924hqtnWNngGAGIXkQXQKWYKmGIHk4dH8JHri_QaALKEYP45OEEK7GTyNtleljl1rC9vEzsTLL4u1fx9bpZvOGitFZ91-oxZ1LSormriy33W8Lp1yW2-bSTxXpfaBmsTLqu9ELTodi0bF5629uam0OnjGwd_0TWGfRo-MqLx-NnzPom_nn64Wn6cXl8vVYn4xlYSl3TTJNYUqT1gCUSKJ1iAFmchMxqgwJJM5QhIzkGJjqFSKAkJDAkqSXCuVEZCcRS8PvtvKeT6E5TlMEAIUAAIDsToQyokN37a2Fu0f7oTl-wXXFly0nZWV5nmKmFFGYZPBVCCSY0kzyHLFSE6pSoPX5ODlf-ltn4_cPtrr-d6t7zkkCGYs4B-Gy_V5rZUMabeiGqnGO40teeF-8gRlEAMcDN4MBq370Wvf8dp6qatKNNr1njOIYYoZ2h316h_y_iwGqhDhd21jXDhW7jz5PCUEEogZDdTsHioMpWsrQx0aG9ZHgrcjQWA6_bsrRO89X31d_z97eT1mXx-xpRZVV3oXyi_UoR-D6QGUrfO-1eYuYwj4ro1u0-C7NuJDGwXZi-P3uRPd9k3yFzyAF1M</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1322080071</pqid></control><display><type>article</type><title>The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>SWEPUB Freely available online</source><source>Free Full-Text Journals in Chemistry</source><creator>Krishnan, Arunkumar ; Almén, Markus Sällman ; Fredriksson, Robert ; Schiöth, Helgi B</creator><contributor>Xue, Chaoyang</contributor><creatorcontrib>Krishnan, Arunkumar ; Almén, Markus Sällman ; Fredriksson, Robert ; Schiöth, Helgi B ; Xue, Chaoyang</creatorcontrib><description>G protein-coupled receptors (GPCRs) in humans are classified into the five main families named Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin according to the GRAFS classification. Previous results show that these mammalian GRAFS families are well represented in the Metazoan lineages, but they have not been shown to be present in Fungi. Here, we systematically mined 79 fungal genomes and provide the first evidence that four of the five main mammalian families of GPCRs, namely Rhodopsin, Adhesion, Glutamate and Frizzled, are present in Fungi and found 142 novel sequences between them. Significantly, we provide strong evidence that the Rhodopsin family emerged from the cAMP receptor family in an event close to the split of Opisthokonts and not in Placozoa, as earlier assumed. The Rhodopsin family then expanded greatly in Metazoans while the cAMP receptor family is found in 3 invertebrate species and lost in the vertebrates. We estimate that the Adhesion and Frizzled families evolved before the split of Unikonts from a common ancestor of all major eukaryotic lineages. Also, the study highlights that the fungal Adhesion receptors do not have N-terminal domains whereas the fungal Glutamate receptors have a broad repertoire of mammalian-like N-terminal domains. Further, mining of the close unicellular relatives of the Metazoan lineage, Salpingoeca rosetta and Capsaspora owczarzaki, obtained a rich group of both the Adhesion and Glutamate families, which in particular provided insight to the early emergence of the N-terminal domains of the Adhesion family. We identified 619 Fungi specific GPCRs across 79 genomes and revealed that Blastocladiomycota and Chytridiomycota phylum have Metazoan-like GPCRs rather than the GPCRs specific for Fungi. Overall, this study provides the first evidence of the presence of four of the five main GRAFS families in Fungi and clarifies the early evolutionary history of the GPCR superfamily.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0029817</identifier><identifier>PMID: 22238661</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adhesion ; Algorithms ; Amino Acid Sequence ; Amino acids ; Amoebozoa ; Animals ; Bioinformatics ; Biology ; Cell Adhesion Molecules - genetics ; Cell Adhesion Molecules - isolation &amp; purification ; Cloning, Molecular ; Cyclic adenosine monophosphate ; Cyclic AMP ; Datasets ; Evolution, Molecular ; Filasterea ; Frizzled protein ; Frizzled Receptors - genetics ; Frizzled Receptors - isolation &amp; purification ; Fungi ; Fungi - genetics ; Fungi - physiology ; G protein-coupled receptors ; Genes, Fungal ; Genome, Fungal ; Genomes ; Genomics ; Glutamate ; Glutamic acid receptors ; Humans ; Hypotheses ; Ligands ; Mammals ; Mammals - genetics ; Metazoa ; Molecular Sequence Data ; Nematoda ; Neurosciences ; Paramecium ; Peptides ; Pharmacology ; Pheromones ; Phylogenetics ; Phylogeny ; Placozoa ; Proteins ; Receptors ; Receptors, G-Protein-Coupled - classification ; Receptors, G-Protein-Coupled - genetics ; Receptors, Glutamate - genetics ; Receptors, Glutamate - isolation &amp; purification ; Rhodopsin ; Rhodopsin - genetics ; Rhodopsin - isolation &amp; purification ; Secretin ; Tetrahymena thermophila ; Trends ; Vertebrates</subject><ispartof>PloS one, 2012-01, Vol.7 (1), p.e29817-e29817</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Krishnan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Krishnan et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c794t-3be81db393123c7ee0405a5f598af75cb22c69046ff8cdd8078298dc7bedd5703</citedby><cites>FETCH-LOGICAL-c794t-3be81db393123c7ee0405a5f598af75cb22c69046ff8cdd8078298dc7bedd5703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3251606/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3251606/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,554,729,782,786,866,887,2104,2930,23873,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22238661$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-172159$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Xue, Chaoyang</contributor><creatorcontrib>Krishnan, Arunkumar</creatorcontrib><creatorcontrib>Almén, Markus Sällman</creatorcontrib><creatorcontrib>Fredriksson, Robert</creatorcontrib><creatorcontrib>Schiöth, Helgi B</creatorcontrib><title>The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>G protein-coupled receptors (GPCRs) in humans are classified into the five main families named Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin according to the GRAFS classification. Previous results show that these mammalian GRAFS families are well represented in the Metazoan lineages, but they have not been shown to be present in Fungi. Here, we systematically mined 79 fungal genomes and provide the first evidence that four of the five main mammalian families of GPCRs, namely Rhodopsin, Adhesion, Glutamate and Frizzled, are present in Fungi and found 142 novel sequences between them. Significantly, we provide strong evidence that the Rhodopsin family emerged from the cAMP receptor family in an event close to the split of Opisthokonts and not in Placozoa, as earlier assumed. The Rhodopsin family then expanded greatly in Metazoans while the cAMP receptor family is found in 3 invertebrate species and lost in the vertebrates. We estimate that the Adhesion and Frizzled families evolved before the split of Unikonts from a common ancestor of all major eukaryotic lineages. Also, the study highlights that the fungal Adhesion receptors do not have N-terminal domains whereas the fungal Glutamate receptors have a broad repertoire of mammalian-like N-terminal domains. Further, mining of the close unicellular relatives of the Metazoan lineage, Salpingoeca rosetta and Capsaspora owczarzaki, obtained a rich group of both the Adhesion and Glutamate families, which in particular provided insight to the early emergence of the N-terminal domains of the Adhesion family. We identified 619 Fungi specific GPCRs across 79 genomes and revealed that Blastocladiomycota and Chytridiomycota phylum have Metazoan-like GPCRs rather than the GPCRs specific for Fungi. Overall, this study provides the first evidence of the presence of four of the five main GRAFS families in Fungi and clarifies the early evolutionary history of the GPCR superfamily.</description><subject>Adhesion</subject><subject>Algorithms</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Amoebozoa</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>Cell Adhesion Molecules - genetics</subject><subject>Cell Adhesion Molecules - isolation &amp; purification</subject><subject>Cloning, Molecular</subject><subject>Cyclic adenosine monophosphate</subject><subject>Cyclic AMP</subject><subject>Datasets</subject><subject>Evolution, Molecular</subject><subject>Filasterea</subject><subject>Frizzled protein</subject><subject>Frizzled Receptors - genetics</subject><subject>Frizzled Receptors - isolation &amp; purification</subject><subject>Fungi</subject><subject>Fungi - genetics</subject><subject>Fungi - physiology</subject><subject>G protein-coupled receptors</subject><subject>Genes, Fungal</subject><subject>Genome, Fungal</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Glutamate</subject><subject>Glutamic acid receptors</subject><subject>Humans</subject><subject>Hypotheses</subject><subject>Ligands</subject><subject>Mammals</subject><subject>Mammals - genetics</subject><subject>Metazoa</subject><subject>Molecular Sequence Data</subject><subject>Nematoda</subject><subject>Neurosciences</subject><subject>Paramecium</subject><subject>Peptides</subject><subject>Pharmacology</subject><subject>Pheromones</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Placozoa</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Receptors, G-Protein-Coupled - classification</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, Glutamate - genetics</subject><subject>Receptors, Glutamate - isolation &amp; purification</subject><subject>Rhodopsin</subject><subject>Rhodopsin - genetics</subject><subject>Rhodopsin - isolation &amp; purification</subject><subject>Secretin</subject><subject>Tetrahymena thermophila</subject><subject>Trends</subject><subject>Vertebrates</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>D8T</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1tv0zAYhiMEYmPwDxBEQgIhtcV2Eh-4QKoKK5UmDZWxW8vxIXFJ4hInHPbrcdtsatAukC9i2c_72nn9fVH0HIIZTAh8t3F924hqtnWNngGAGIXkQXQKWYKmGIHk4dH8JHri_QaALKEYP45OEEK7GTyNtleljl1rC9vEzsTLL4u1fx9bpZvOGitFZ91-oxZ1LSormriy33W8Lp1yW2-bSTxXpfaBmsTLqu9ELTodi0bF5629uam0OnjGwd_0TWGfRo-MqLx-NnzPom_nn64Wn6cXl8vVYn4xlYSl3TTJNYUqT1gCUSKJ1iAFmchMxqgwJJM5QhIzkGJjqFSKAkJDAkqSXCuVEZCcRS8PvtvKeT6E5TlMEAIUAAIDsToQyokN37a2Fu0f7oTl-wXXFly0nZWV5nmKmFFGYZPBVCCSY0kzyHLFSE6pSoPX5ODlf-ltn4_cPtrr-d6t7zkkCGYs4B-Gy_V5rZUMabeiGqnGO40teeF-8gRlEAMcDN4MBq370Wvf8dp6qatKNNr1njOIYYoZ2h316h_y_iwGqhDhd21jXDhW7jz5PCUEEogZDdTsHioMpWsrQx0aG9ZHgrcjQWA6_bsrRO89X31d_z97eT1mXx-xpRZVV3oXyi_UoR-D6QGUrfO-1eYuYwj4ro1u0-C7NuJDGwXZi-P3uRPd9k3yFzyAF1M</recordid><startdate>20120104</startdate><enddate>20120104</enddate><creator>Krishnan, Arunkumar</creator><creator>Almén, Markus Sällman</creator><creator>Fredriksson, Robert</creator><creator>Schiöth, Helgi B</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>ACNBI</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>DF2</scope><scope>ZZAVC</scope><scope>DOA</scope></search><sort><creationdate>20120104</creationdate><title>The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi</title><author>Krishnan, Arunkumar ; Almén, Markus Sällman ; Fredriksson, Robert ; Schiöth, Helgi B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c794t-3be81db393123c7ee0405a5f598af75cb22c69046ff8cdd8078298dc7bedd5703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adhesion</topic><topic>Algorithms</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Amoebozoa</topic><topic>Animals</topic><topic>Bioinformatics</topic><topic>Biology</topic><topic>Cell Adhesion Molecules - genetics</topic><topic>Cell Adhesion Molecules - isolation &amp; purification</topic><topic>Cloning, Molecular</topic><topic>Cyclic adenosine monophosphate</topic><topic>Cyclic AMP</topic><topic>Datasets</topic><topic>Evolution, Molecular</topic><topic>Filasterea</topic><topic>Frizzled protein</topic><topic>Frizzled Receptors - genetics</topic><topic>Frizzled Receptors - isolation &amp; purification</topic><topic>Fungi</topic><topic>Fungi - genetics</topic><topic>Fungi - physiology</topic><topic>G protein-coupled receptors</topic><topic>Genes, Fungal</topic><topic>Genome, Fungal</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Glutamate</topic><topic>Glutamic acid receptors</topic><topic>Humans</topic><topic>Hypotheses</topic><topic>Ligands</topic><topic>Mammals</topic><topic>Mammals - genetics</topic><topic>Metazoa</topic><topic>Molecular Sequence Data</topic><topic>Nematoda</topic><topic>Neurosciences</topic><topic>Paramecium</topic><topic>Peptides</topic><topic>Pharmacology</topic><topic>Pheromones</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Placozoa</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Receptors, G-Protein-Coupled - classification</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, Glutamate - genetics</topic><topic>Receptors, Glutamate - isolation &amp; purification</topic><topic>Rhodopsin</topic><topic>Rhodopsin - genetics</topic><topic>Rhodopsin - isolation &amp; purification</topic><topic>Secretin</topic><topic>Tetrahymena thermophila</topic><topic>Trends</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krishnan, Arunkumar</creatorcontrib><creatorcontrib>Almén, Markus Sällman</creatorcontrib><creatorcontrib>Fredriksson, Robert</creatorcontrib><creatorcontrib>Schiöth, Helgi B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SWEPUB Uppsala universitet full text</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Uppsala universitet</collection><collection>SwePub Articles full text</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krishnan, Arunkumar</au><au>Almén, Markus Sällman</au><au>Fredriksson, Robert</au><au>Schiöth, Helgi B</au><au>Xue, Chaoyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-01-04</date><risdate>2012</risdate><volume>7</volume><issue>1</issue><spage>e29817</spage><epage>e29817</epage><pages>e29817-e29817</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>G protein-coupled receptors (GPCRs) in humans are classified into the five main families named Glutamate, Rhodopsin, Adhesion, Frizzled and Secretin according to the GRAFS classification. Previous results show that these mammalian GRAFS families are well represented in the Metazoan lineages, but they have not been shown to be present in Fungi. Here, we systematically mined 79 fungal genomes and provide the first evidence that four of the five main mammalian families of GPCRs, namely Rhodopsin, Adhesion, Glutamate and Frizzled, are present in Fungi and found 142 novel sequences between them. Significantly, we provide strong evidence that the Rhodopsin family emerged from the cAMP receptor family in an event close to the split of Opisthokonts and not in Placozoa, as earlier assumed. The Rhodopsin family then expanded greatly in Metazoans while the cAMP receptor family is found in 3 invertebrate species and lost in the vertebrates. We estimate that the Adhesion and Frizzled families evolved before the split of Unikonts from a common ancestor of all major eukaryotic lineages. Also, the study highlights that the fungal Adhesion receptors do not have N-terminal domains whereas the fungal Glutamate receptors have a broad repertoire of mammalian-like N-terminal domains. Further, mining of the close unicellular relatives of the Metazoan lineage, Salpingoeca rosetta and Capsaspora owczarzaki, obtained a rich group of both the Adhesion and Glutamate families, which in particular provided insight to the early emergence of the N-terminal domains of the Adhesion family. We identified 619 Fungi specific GPCRs across 79 genomes and revealed that Blastocladiomycota and Chytridiomycota phylum have Metazoan-like GPCRs rather than the GPCRs specific for Fungi. Overall, this study provides the first evidence of the presence of four of the five main GRAFS families in Fungi and clarifies the early evolutionary history of the GPCR superfamily.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22238661</pmid><doi>10.1371/journal.pone.0029817</doi><tpages>e29817</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2012-01, Vol.7 (1), p.e29817-e29817
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1322080071
source Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; SWEPUB Freely available online; Free Full-Text Journals in Chemistry
subjects Adhesion
Algorithms
Amino Acid Sequence
Amino acids
Amoebozoa
Animals
Bioinformatics
Biology
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - isolation & purification
Cloning, Molecular
Cyclic adenosine monophosphate
Cyclic AMP
Datasets
Evolution, Molecular
Filasterea
Frizzled protein
Frizzled Receptors - genetics
Frizzled Receptors - isolation & purification
Fungi
Fungi - genetics
Fungi - physiology
G protein-coupled receptors
Genes, Fungal
Genome, Fungal
Genomes
Genomics
Glutamate
Glutamic acid receptors
Humans
Hypotheses
Ligands
Mammals
Mammals - genetics
Metazoa
Molecular Sequence Data
Nematoda
Neurosciences
Paramecium
Peptides
Pharmacology
Pheromones
Phylogenetics
Phylogeny
Placozoa
Proteins
Receptors
Receptors, G-Protein-Coupled - classification
Receptors, G-Protein-Coupled - genetics
Receptors, Glutamate - genetics
Receptors, Glutamate - isolation & purification
Rhodopsin
Rhodopsin - genetics
Rhodopsin - isolation & purification
Secretin
Tetrahymena thermophila
Trends
Vertebrates
title The origin of GPCRs: identification of mammalian like Rhodopsin, Adhesion, Glutamate and Frizzled GPCRs in fungi
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-09T14%3A08%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20origin%20of%20GPCRs:%20identification%20of%20mammalian%20like%20Rhodopsin,%20Adhesion,%20Glutamate%20and%20Frizzled%20GPCRs%20in%20fungi&rft.jtitle=PloS%20one&rft.au=Krishnan,%20Arunkumar&rft.date=2012-01-04&rft.volume=7&rft.issue=1&rft.spage=e29817&rft.epage=e29817&rft.pages=e29817-e29817&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0029817&rft_dat=%3Cgale_plos_%3EA477171698%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1322080071&rft_id=info:pmid/22238661&rft_galeid=A477171698&rft_doaj_id=oai_doaj_org_article_b429fdfd6f514a27b6c8519bd97b88d4&rfr_iscdi=true