Automated reporter quantification in vivo: high-throughput screening method for reporter-based assays in zebrafish

Reporter-based assays underlie many high-throughput screening (HTS) platforms, but most are limited to in vitro applications. Here, we report a simple whole-organism HTS method for quantifying changes in reporter intensity in individual zebrafish over time termed, Automated Reporter Quantification i...

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Veröffentlicht in:	PloS one 2012-01, Vol.7 (1), p.e29916-e29916
Hauptverfasser:	Walker, Steven L, Ariga, Junko, Mathias, Jonathan R, Coothankandaswamy, Veena, Xie, Xiayang, Distel, Martin, Köster, Reinhard W, Parsons, Michael J, Bhalla, Kapil N, Saxena, Meera T, Mumm, Jeff S
Format:	Artikel
Sprache:	eng
Schlagworte:	Advantages Animals Animals, Genetically Modified Assaying Automation - methods Bacterial Proteins - genetics Bacterial Proteins - metabolism Beta cells Bioassays Biology Cancer Cell number Cellular biology Danio rerio Data acquisition Drug discovery Embryo, Nonmammalian Embryos Fluorescence Gene Dosage - physiology Gene Expression Profiling - methods Gene Expression Regulation, Developmental Genes, Reporter Genetic engineering Genomics Health sciences High-throughput screening High-Throughput Screening Assays - methods Image Processing, Computer-Assisted - methods In vivo methods and tests Journalists Laboratory animals Larvae Luminescent Proteins - genetics Luminescent Proteins - metabolism Medical screening Metabolism Methods Notch protein Osmolar Concentration Oxygen Pancreas Pharmaceuticals Photoreceptors R&D Reactive oxygen species Regeneration Reproducibility of Results Research & development Signaling Validation Studies as Topic Zebrafish Zebrafish - embryology Zebrafish - genetics
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