Improvement of the trivalent inactivated flu vaccine using PapMV nanoparticles
Commercial seasonal flu vaccines induce production of antibodies directed mostly towards hemaglutinin (HA). Because HA changes rapidly in the circulating virus, the protection remains partial. Several conserved viral proteins, e.g., nucleocapsid (NP) and matrix proteins (M1), are present in the vacc...
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| Veröffentlicht in: | PloS one 2011-06, Vol.6 (6), p.e21522 |
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| creator | Savard, Christian Guérin, Annie Drouin, Karine Bolduc, Marilène Laliberté-Gagné, Marie-Eve Dumas, Marie-Christine Majeau, Nathalie Leclerc, Denis |
| description | Commercial seasonal flu vaccines induce production of antibodies directed mostly towards hemaglutinin (HA). Because HA changes rapidly in the circulating virus, the protection remains partial. Several conserved viral proteins, e.g., nucleocapsid (NP) and matrix proteins (M1), are present in the vaccine, but are not immunogenic. To improve the protection provided by these vaccines, we used nanoparticles made of the coat protein of a plant virus (papaya mosaic virus; PapMV) as an adjuvant. Immunization of mice and ferrets with the adjuvanted formulation increased the magnitude and breadth of the humoral response to NP and to highly conserved regions of HA. They also triggered a cellular mediated immune response to NP and M1, and long-lasting protection in animals challenged with a heterosubtypic influenza strain (WSN/33). Thus, seasonal flu vaccine adjuvanted with PapMV nanoparticles can induce universal protection to influenza, which is a major advancement when facing a pandemic. |
| doi_str_mv | 10.1371/journal.pone.0021522 |
| format | Article |
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Because HA changes rapidly in the circulating virus, the protection remains partial. Several conserved viral proteins, e.g., nucleocapsid (NP) and matrix proteins (M1), are present in the vaccine, but are not immunogenic. To improve the protection provided by these vaccines, we used nanoparticles made of the coat protein of a plant virus (papaya mosaic virus; PapMV) as an adjuvant. Immunization of mice and ferrets with the adjuvanted formulation increased the magnitude and breadth of the humoral response to NP and to highly conserved regions of HA. They also triggered a cellular mediated immune response to NP and M1, and long-lasting protection in animals challenged with a heterosubtypic influenza strain (WSN/33). Thus, seasonal flu vaccine adjuvanted with PapMV nanoparticles can induce universal protection to influenza, which is a major advancement when facing a pandemic.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0021522</identifier><identifier>PMID: 21747909</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies ; Avian flu ; Biological Transport ; Biology ; Carica - virology ; Coat protein ; Cytokines ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; Hemagglutinin Glycoproteins, Influenza Virus - chemistry ; Hemagglutinin Glycoproteins, Influenza Virus - immunology ; Humans ; Immune response ; Immune response (humoral) ; Immune system ; Immunity, Humoral - immunology ; Immunization ; Immunogenicity ; Immunology ; Infections ; Infectious diseases ; Influenza ; Influenza A Virus, H1N1 Subtype - immunology ; Influenza vaccines ; Influenza Vaccines - chemistry ; Influenza Vaccines - immunology ; Influenza Vaccines - metabolism ; Interferon-gamma - immunology ; Interferon-gamma - secretion ; Lymph Nodes - immunology ; Lymph Nodes - metabolism ; Lymphatic system ; Lymphocytes ; Male ; Medicine ; Mice ; Molecular Sequence Data ; Mosaic Viruses - chemistry ; Mosaic Viruses - immunology ; Mosaic Viruses - metabolism ; Nanoparticles ; Nanoparticles - virology ; Nucleocapsids ; Pain ; Pandemics ; Plant viruses ; Proteins ; Seasons ; T-Lymphocytes - immunology ; T-Lymphocytes - secretion ; Tumor necrosis factor-TNF ; Vaccines ; Vaccines, Inactivated - chemistry ; Vaccines, Inactivated - immunology ; Vaccines, Inactivated - metabolism ; Viral infections ; Viral proteins ; Viruses ; Wildlife conservation</subject><ispartof>PloS one, 2011-06, Vol.6 (6), p.e21522</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Savard et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Because HA changes rapidly in the circulating virus, the protection remains partial. Several conserved viral proteins, e.g., nucleocapsid (NP) and matrix proteins (M1), are present in the vaccine, but are not immunogenic. To improve the protection provided by these vaccines, we used nanoparticles made of the coat protein of a plant virus (papaya mosaic virus; PapMV) as an adjuvant. Immunization of mice and ferrets with the adjuvanted formulation increased the magnitude and breadth of the humoral response to NP and to highly conserved regions of HA. They also triggered a cellular mediated immune response to NP and M1, and long-lasting protection in animals challenged with a heterosubtypic influenza strain (WSN/33). 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immunology</subject><subject>Influenza vaccines</subject><subject>Influenza Vaccines - chemistry</subject><subject>Influenza Vaccines - immunology</subject><subject>Influenza Vaccines - metabolism</subject><subject>Interferon-gamma - immunology</subject><subject>Interferon-gamma - secretion</subject><subject>Lymph Nodes - immunology</subject><subject>Lymph Nodes - metabolism</subject><subject>Lymphatic system</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Medicine</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Mosaic Viruses - chemistry</subject><subject>Mosaic Viruses - immunology</subject><subject>Mosaic Viruses - metabolism</subject><subject>Nanoparticles</subject><subject>Nanoparticles - virology</subject><subject>Nucleocapsids</subject><subject>Pain</subject><subject>Pandemics</subject><subject>Plant viruses</subject><subject>Proteins</subject><subject>Seasons</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - secretion</subject><subject>Tumor necrosis factor-TNF</subject><subject>Vaccines</subject><subject>Vaccines, Inactivated - chemistry</subject><subject>Vaccines, Inactivated - immunology</subject><subject>Vaccines, Inactivated - metabolism</subject><subject>Viral infections</subject><subject>Viral proteins</subject><subject>Viruses</subject><subject>Wildlife conservation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLguDFjDlJk7Y3wrL4MbC64sfehjRNZjK0SU3SYfffm3G6yxQUpBdpTp7z5vDmzbLngJZASni7daO3olsOzqolQhgoxg-yU6gJXjCMyMOj_5PsSQhbhCipGHucnWAoi7JG9Wn2ZdUP3u1Ur2zMnc7jRuXRm53o9gVjhYxpE1Wb627Md0JKY1U-BmPX-VcxfL7OrbBuED4a2anwNHukRRfUs2k9y35-eP_j4tPi8urj6uL8ciFZDXFR6Lah0KhK60YQUjAoUUnqom7ShLQs6pbUkkHdMGBYF6CAIlmVtCEaKYQ0OcteHnSHzgU-WRE4EKgxxrRkiVgdiNaJLR-86YW_5U4Y_qfg_JpPQ3MNlDa01Q2luJACCQ1YU6YIVhUAqKT1brptbHrVymSNF91MdH5izYav3Y4TwKzCZRJ4NQl492tUIf5j5IlaJ_e5sdolMdmbIPl5UbIqvR3BiVr-hUpfq3ojUxi0SfVZw5tZQ2KiuolrMYbAV9-__T97dT1nXx-xGyW6uAmuG6NxNszB4gBK70LwSt87B4jvs3znBt9nmU9ZTm0vjl2_b7oLL_kNR8_uZg</recordid><startdate>20110629</startdate><enddate>20110629</enddate><creator>Savard, Christian</creator><creator>Guérin, Annie</creator><creator>Drouin, Karine</creator><creator>Bolduc, Marilène</creator><creator>Laliberté-Gagné, Marie-Eve</creator><creator>Dumas, Marie-Christine</creator><creator>Majeau, Nathalie</creator><creator>Leclerc, Denis</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110629</creationdate><title>Improvement of the trivalent inactivated flu vaccine using PapMV nanoparticles</title><author>Savard, Christian ; Guérin, Annie ; Drouin, Karine ; Bolduc, Marilène ; Laliberté-Gagné, Marie-Eve ; Dumas, Marie-Christine ; Majeau, Nathalie ; Leclerc, Denis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-4fdb51be8ffba334617073949b5385749d39c619b6162f41e150c875b3f0e00f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Avian flu</topic><topic>Biological Transport</topic><topic>Biology</topic><topic>Carica - virology</topic><topic>Coat protein</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - chemistry</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - immunology</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune response (humoral)</topic><topic>Immune system</topic><topic>Immunity, Humoral - immunology</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunology</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Influenza</topic><topic>Influenza A Virus, H1N1 Subtype - immunology</topic><topic>Influenza vaccines</topic><topic>Influenza Vaccines - chemistry</topic><topic>Influenza Vaccines - immunology</topic><topic>Influenza Vaccines - metabolism</topic><topic>Interferon-gamma - immunology</topic><topic>Interferon-gamma - secretion</topic><topic>Lymph Nodes - immunology</topic><topic>Lymph Nodes - metabolism</topic><topic>Lymphatic system</topic><topic>Lymphocytes</topic><topic>Male</topic><topic>Medicine</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Mosaic Viruses - 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Because HA changes rapidly in the circulating virus, the protection remains partial. Several conserved viral proteins, e.g., nucleocapsid (NP) and matrix proteins (M1), are present in the vaccine, but are not immunogenic. To improve the protection provided by these vaccines, we used nanoparticles made of the coat protein of a plant virus (papaya mosaic virus; PapMV) as an adjuvant. Immunization of mice and ferrets with the adjuvanted formulation increased the magnitude and breadth of the humoral response to NP and to highly conserved regions of HA. They also triggered a cellular mediated immune response to NP and M1, and long-lasting protection in animals challenged with a heterosubtypic influenza strain (WSN/33). 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| source | MEDLINE; Public Library of Science; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Amino Acid Sequence Animals Antibodies Avian flu Biological Transport Biology Carica - virology Coat protein Cytokines Cytotoxicity Deoxyribonucleic acid DNA Hemagglutinin Glycoproteins, Influenza Virus - chemistry Hemagglutinin Glycoproteins, Influenza Virus - immunology Humans Immune response Immune response (humoral) Immune system Immunity, Humoral - immunology Immunization Immunogenicity Immunology Infections Infectious diseases Influenza Influenza A Virus, H1N1 Subtype - immunology Influenza vaccines Influenza Vaccines - chemistry Influenza Vaccines - immunology Influenza Vaccines - metabolism Interferon-gamma - immunology Interferon-gamma - secretion Lymph Nodes - immunology Lymph Nodes - metabolism Lymphatic system Lymphocytes Male Medicine Mice Molecular Sequence Data Mosaic Viruses - chemistry Mosaic Viruses - immunology Mosaic Viruses - metabolism Nanoparticles Nanoparticles - virology Nucleocapsids Pain Pandemics Plant viruses Proteins Seasons T-Lymphocytes - immunology T-Lymphocytes - secretion Tumor necrosis factor-TNF Vaccines Vaccines, Inactivated - chemistry Vaccines, Inactivated - immunology Vaccines, Inactivated - metabolism Viral infections Viral proteins Viruses Wildlife conservation |
| title | Improvement of the trivalent inactivated flu vaccine using PapMV nanoparticles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T12%3A46%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improvement%20of%20the%20trivalent%20inactivated%20flu%20vaccine%20using%20PapMV%20nanoparticles&rft.jtitle=PloS%20one&rft.au=Savard,%20Christian&rft.date=2011-06-29&rft.volume=6&rft.issue=6&rft.spage=e21522&rft.pages=e21522-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0021522&rft_dat=%3Cgale_plos_%3EA476886632%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1319222576&rft_id=info:pmid/21747909&rft_galeid=A476886632&rft_doaj_id=oai_doaj_org_article_f155b5dfb5524ca0af12f56e32e8111e&rfr_iscdi=true |