Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control

Green fluorescent protein (GFP) has undergone a long history of optimization to become one of the most popular proteins in all of cell biology. It is thermally and chemically robust and produces a pronounced fluorescent phenotype when expressed in cells of all types. Recently, a superfolder GFP was...

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Veröffentlicht in:	PloS one 2008-06, Vol.3 (6), p.e2351-e2351
Hauptverfasser:	Fisher, Adam C, DeLisa, Matthew P
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Bacteria Biochemistry Biochemistry/Protein Folding Biotechnology Biotechnology/Applied Microbiology Biotechnology/Bioengineering Biotechnology/Protein Chemistry and Proteomics Cells (Biology) Combinatorial analysis Cytoplasm Denaturation E coli Engineering Escherichia coli Evolution Fluorescence Folding Gene expression Genotype Green fluorescent protein Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Kinetics Microbiology Microbiology/Applied Microbiology Microbiology/Microbial Physiology and Metabolism Mutagenesis Mutation Optimization Peptides Phenotype Polymerase chain reaction Protein denaturation Protein Folding Proteins Quality Control Secretion Translocase
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