Quantitative and rapid DNA detection by laser transmission spectroscopy
Laser transmission spectroscopy (LTS) is a quantitative and rapid in vitro technique for measuring the size, shape, and number of nanoparticles in suspension. Here we report on the application of LTS as a novel detection method for species-specific DNA where the presence of one invasive species was...
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| Veröffentlicht in: | PloS one 2011-12, Vol.6 (12), p.e29224 |
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| creator | Li, Frank Mahon, Andrew R Barnes, Matthew A Feder, Jeffery Lodge, David M Hwang, Ching-Ting Schafer, Robert Ruggiero, Steven T Tanner, Carol E |
| description | Laser transmission spectroscopy (LTS) is a quantitative and rapid in vitro technique for measuring the size, shape, and number of nanoparticles in suspension. Here we report on the application of LTS as a novel detection method for species-specific DNA where the presence of one invasive species was differentiated from a closely related invasive sister species. The method employs carboxylated polystyrene nanoparticles functionalized with short DNA fragments that are complimentary to a specific target DNA sequence. In solution, the DNA strands containing targets bind to the tags resulting in a sizable increase in the nanoparticle diameter, which is rapidly and quantitatively measured using LTS. DNA strands that do not contain the target sequence do not bind and produce no size change of the carboxylated beads. The results show that LTS has the potential to become a quantitative and rapid DNA detection method suitable for many real-world applications. |
| doi_str_mv | 10.1371/journal.pone.0029224 |
| format | Article |
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Here we report on the application of LTS as a novel detection method for species-specific DNA where the presence of one invasive species was differentiated from a closely related invasive sister species. The method employs carboxylated polystyrene nanoparticles functionalized with short DNA fragments that are complimentary to a specific target DNA sequence. In solution, the DNA strands containing targets bind to the tags resulting in a sizable increase in the nanoparticle diameter, which is rapidly and quantitatively measured using LTS. DNA strands that do not contain the target sequence do not bind and produce no size change of the carboxylated beads. The results show that LTS has the potential to become a quantitative and rapid DNA detection method suitable for many real-world applications.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0029224</identifier><identifier>PMID: 22195026</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Beads ; Biology ; Biomarkers - metabolism ; Bivalvia - genetics ; Carbon ; Deoxyribonucleic acid ; DNA ; DNA - analysis ; DNA - genetics ; DNA sequencing ; Endangered & extinct species ; Extinction ; Hydrogels ; In vitro methods and tests ; Internet ; Introduced species ; Invasive species ; Lasers ; Light ; Materials Science ; Microscopy ; Nanoparticles ; Nanostructures - chemistry ; Nanowires ; Nonnative species ; Nucleotide sequence ; Particle size ; Physics ; Polymerase Chain Reaction ; Polystyrene ; Polystyrene resins ; Scattering, Radiation ; Sensors ; Sibling species ; Species Specificity ; Spectroscopy ; Spectrum Analysis - methods ; Strands</subject><ispartof>PloS one, 2011-12, Vol.6 (12), p.e29224</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Li et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-27b219d8fafd4851d0712a43421090392155adea40589dd445b409f9836e41b53</citedby><cites>FETCH-LOGICAL-c691t-27b219d8fafd4851d0712a43421090392155adea40589dd445b409f9836e41b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241715/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241715/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79472,79473</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22195026$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wanunu, Meni</contributor><creatorcontrib>Li, Frank</creatorcontrib><creatorcontrib>Mahon, Andrew R</creatorcontrib><creatorcontrib>Barnes, Matthew A</creatorcontrib><creatorcontrib>Feder, Jeffery</creatorcontrib><creatorcontrib>Lodge, David M</creatorcontrib><creatorcontrib>Hwang, Ching-Ting</creatorcontrib><creatorcontrib>Schafer, Robert</creatorcontrib><creatorcontrib>Ruggiero, Steven T</creatorcontrib><creatorcontrib>Tanner, Carol E</creatorcontrib><title>Quantitative and rapid DNA detection by laser transmission spectroscopy</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Laser transmission spectroscopy (LTS) is a quantitative and rapid in vitro technique for measuring the size, shape, and number of nanoparticles in suspension. Here we report on the application of LTS as a novel detection method for species-specific DNA where the presence of one invasive species was differentiated from a closely related invasive sister species. The method employs carboxylated polystyrene nanoparticles functionalized with short DNA fragments that are complimentary to a specific target DNA sequence. In solution, the DNA strands containing targets bind to the tags resulting in a sizable increase in the nanoparticle diameter, which is rapidly and quantitatively measured using LTS. DNA strands that do not contain the target sequence do not bind and produce no size change of the carboxylated beads. The results show that LTS has the potential to become a quantitative and rapid DNA detection method suitable for many real-world applications.</description><subject>Animals</subject><subject>Beads</subject><subject>Biology</subject><subject>Biomarkers - metabolism</subject><subject>Bivalvia - genetics</subject><subject>Carbon</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA - genetics</subject><subject>DNA sequencing</subject><subject>Endangered & extinct species</subject><subject>Extinction</subject><subject>Hydrogels</subject><subject>In vitro methods and tests</subject><subject>Internet</subject><subject>Introduced species</subject><subject>Invasive species</subject><subject>Lasers</subject><subject>Light</subject><subject>Materials Science</subject><subject>Microscopy</subject><subject>Nanoparticles</subject><subject>Nanostructures - chemistry</subject><subject>Nanowires</subject><subject>Nonnative species</subject><subject>Nucleotide sequence</subject><subject>Particle size</subject><subject>Physics</subject><subject>Polymerase Chain Reaction</subject><subject>Polystyrene</subject><subject>Polystyrene resins</subject><subject>Scattering, Radiation</subject><subject>Sensors</subject><subject>Sibling species</subject><subject>Species Specificity</subject><subject>Spectroscopy</subject><subject>Spectrum Analysis - 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Here we report on the application of LTS as a novel detection method for species-specific DNA where the presence of one invasive species was differentiated from a closely related invasive sister species. The method employs carboxylated polystyrene nanoparticles functionalized with short DNA fragments that are complimentary to a specific target DNA sequence. In solution, the DNA strands containing targets bind to the tags resulting in a sizable increase in the nanoparticle diameter, which is rapidly and quantitatively measured using LTS. DNA strands that do not contain the target sequence do not bind and produce no size change of the carboxylated beads. The results show that LTS has the potential to become a quantitative and rapid DNA detection method suitable for many real-world applications.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22195026</pmid><doi>10.1371/journal.pone.0029224</doi><tpages>e29224</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Beads Biology Biomarkers - metabolism Bivalvia - genetics Carbon Deoxyribonucleic acid DNA DNA - analysis DNA - genetics DNA sequencing Endangered & extinct species Extinction Hydrogels In vitro methods and tests Internet Introduced species Invasive species Lasers Light Materials Science Microscopy Nanoparticles Nanostructures - chemistry Nanowires Nonnative species Nucleotide sequence Particle size Physics Polymerase Chain Reaction Polystyrene Polystyrene resins Scattering, Radiation Sensors Sibling species Species Specificity Spectroscopy Spectrum Analysis - methods Strands |
| title | Quantitative and rapid DNA detection by laser transmission spectroscopy |
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