Cultivation of human corneal endothelial cells isolated from paired donor corneas

Consistent expansion of human corneal endothelial cells (hCECs) is critical in the development of tissue engineered endothelial constructs. However, a wide range of complex culture media, developed from different basal media have been reported in the propagation of hCECs, some with more success than...

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Veröffentlicht in:	PloS one 2011-12, Vol.6 (12), p.e28310-e28310
Hauptverfasser:	Peh, Gary S L, Toh, Kah-Peng, Wu, Fei-Yi, Tan, Donald T, Mehta, Jodhbir S
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aging Analysis Biology Cell culture Cell Culture Techniques - methods Cell growth Cell proliferation Cell Proliferation - drug effects Cell Separation - methods Cell Shape - drug effects Cells, Cultured Child Cornea Cultivation Culture media Culture Media - pharmacology Endothelial cells Endothelial Cells - cytology Endothelial Cells - drug effects Endothelium Endothelium, Corneal - cytology Female Humans Hydration Ion Channels - metabolism Male Markers Medicine Membrane Proteins - metabolism Morphology Na+/K+-exchanging ATPase Phosphoproteins - metabolism Physical characteristics Potassium Propagation Sodium-Potassium-Exchanging ATPase - metabolism Tight Junctions - drug effects Tight Junctions - metabolism Tissue Donors Transplants & implants Variability Young Adult Zonula Occludens-1 Protein
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