NASA-approved rotary bioreactor enhances proliferation of human epidermal stem cells and supports formation of 3D epidermis-like structure
The skin is susceptible to different injuries and diseases. One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a "stimulatory"...
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| creator | Lei, Xiao-hua Ning, Li-na Cao, Yu-jing Liu, Shuang Zhang, Shou-bing Qiu, Zhi-fang Hu, Hui-min Zhang, Hui-shan Liu, Shu Duan, En-kui |
| description | The skin is susceptible to different injuries and diseases. One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a "stimulatory" environment for the proliferation and differentiation of stem cells. Here, we employed the NASA-approved rotary bioreactor to investigate the proliferation and differentiation of human epidermal stem cells (hEpSCs). hEpSCs were isolated from children foreskins and enriched by collecting epidermal stem cell colonies. Cytodex-3 micro-carriers and hEpSCs were co-cultured in the rotary bioreactor and 6-well dish for 15 days. The result showed that hEpSCs cultured in rotary bioreactor exhibited enhanced proliferation and viability surpassing those cultured in static conditions. Additionally, immunostaining analysis confirmed higher percentage of ki67 positive cells in rotary bioreactor compared with the static culture. In contrast, comparing with static culture, cells in the rotary bioreactor displayed a low expression of involucrin at day 10. Histological analysis revealed that cells cultured in rotary bioreactor aggregated on the micro-carriers and formed multilayer 3D epidermis structures. In conclusion, our research suggests that NASA-approved rotary bioreactor can support the proliferation of hEpSCs and provide a strategy to form multilayer epidermis structure. |
| doi_str_mv | 10.1371/journal.pone.0026603 |
| format | Article |
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One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a "stimulatory" environment for the proliferation and differentiation of stem cells. Here, we employed the NASA-approved rotary bioreactor to investigate the proliferation and differentiation of human epidermal stem cells (hEpSCs). hEpSCs were isolated from children foreskins and enriched by collecting epidermal stem cell colonies. Cytodex-3 micro-carriers and hEpSCs were co-cultured in the rotary bioreactor and 6-well dish for 15 days. The result showed that hEpSCs cultured in rotary bioreactor exhibited enhanced proliferation and viability surpassing those cultured in static conditions. Additionally, immunostaining analysis confirmed higher percentage of ki67 positive cells in rotary bioreactor compared with the static culture. In contrast, comparing with static culture, cells in the rotary bioreactor displayed a low expression of involucrin at day 10. Histological analysis revealed that cells cultured in rotary bioreactor aggregated on the micro-carriers and formed multilayer 3D epidermis structures. In conclusion, our research suggests that NASA-approved rotary bioreactor can support the proliferation of hEpSCs and provide a strategy to form multilayer epidermis structure.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0026603</identifier><identifier>PMID: 22096490</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Biology ; Biomedical materials ; Bioreactors ; Bones ; Carriers ; Cell culture ; Cell Culture Techniques ; Cell cycle ; Cell Differentiation - physiology ; Cell growth ; Cell Proliferation ; Children ; Colonies ; Differentiation ; Disease susceptibility ; Efficiency ; Epidermis ; Epidermis - cytology ; Epidermis - metabolism ; FDA approval ; Fluorescent Antibody Technique ; Humans ; Integrin beta1 - metabolism ; Laboratories ; Lei, Li ; Medicine ; Microgravity ; R&D ; Research & development ; Skin ; Stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Studies ; Tissue engineering ; Viability ; Wound healing ; Zoology</subject><ispartof>PloS one, 2011-11, Vol.6 (11), p.e26603-e26603</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Lei et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Lei et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c757t-3b309c6242fe2ed2d5bdfc3a2fff71b4dd6d3ee9c8b57acdb17aa65bd4e1b2cb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212516/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212516/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22096490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lei, Xiao-hua</creatorcontrib><creatorcontrib>Ning, Li-na</creatorcontrib><creatorcontrib>Cao, Yu-jing</creatorcontrib><creatorcontrib>Liu, Shuang</creatorcontrib><creatorcontrib>Zhang, Shou-bing</creatorcontrib><creatorcontrib>Qiu, Zhi-fang</creatorcontrib><creatorcontrib>Hu, Hui-min</creatorcontrib><creatorcontrib>Zhang, Hui-shan</creatorcontrib><creatorcontrib>Liu, Shu</creatorcontrib><creatorcontrib>Duan, En-kui</creatorcontrib><title>NASA-approved rotary bioreactor enhances proliferation of human epidermal stem cells and supports formation of 3D epidermis-like structure</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The skin is susceptible to different injuries and diseases. One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a "stimulatory" environment for the proliferation and differentiation of stem cells. Here, we employed the NASA-approved rotary bioreactor to investigate the proliferation and differentiation of human epidermal stem cells (hEpSCs). hEpSCs were isolated from children foreskins and enriched by collecting epidermal stem cell colonies. Cytodex-3 micro-carriers and hEpSCs were co-cultured in the rotary bioreactor and 6-well dish for 15 days. The result showed that hEpSCs cultured in rotary bioreactor exhibited enhanced proliferation and viability surpassing those cultured in static conditions. Additionally, immunostaining analysis confirmed higher percentage of ki67 positive cells in rotary bioreactor compared with the static culture. In contrast, comparing with static culture, cells in the rotary bioreactor displayed a low expression of involucrin at day 10. Histological analysis revealed that cells cultured in rotary bioreactor aggregated on the micro-carriers and formed multilayer 3D epidermis structures. In conclusion, our research suggests that NASA-approved rotary bioreactor can support the proliferation of hEpSCs and provide a strategy to form multilayer epidermis structure.</description><subject>Analysis</subject><subject>Biology</subject><subject>Biomedical materials</subject><subject>Bioreactors</subject><subject>Bones</subject><subject>Carriers</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell cycle</subject><subject>Cell Differentiation - physiology</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Children</subject><subject>Colonies</subject><subject>Differentiation</subject><subject>Disease susceptibility</subject><subject>Efficiency</subject><subject>Epidermis</subject><subject>Epidermis - cytology</subject><subject>Epidermis - metabolism</subject><subject>FDA approval</subject><subject>Fluorescent Antibody Technique</subject><subject>Humans</subject><subject>Integrin beta1 - metabolism</subject><subject>Laboratories</subject><subject>Lei, Li</subject><subject>Medicine</subject><subject>Microgravity</subject><subject>R&D</subject><subject>Research & development</subject><subject>Skin</subject><subject>Stem cells</subject><subject>Stem Cells - 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One major obstacle in skin tissue engineering is how to develop functional three-dimensional (3D) substitute for damaged skin. Previous studies have proved a 3D dynamic simulated microgravity (SMG) culture system as a "stimulatory" environment for the proliferation and differentiation of stem cells. Here, we employed the NASA-approved rotary bioreactor to investigate the proliferation and differentiation of human epidermal stem cells (hEpSCs). hEpSCs were isolated from children foreskins and enriched by collecting epidermal stem cell colonies. Cytodex-3 micro-carriers and hEpSCs were co-cultured in the rotary bioreactor and 6-well dish for 15 days. The result showed that hEpSCs cultured in rotary bioreactor exhibited enhanced proliferation and viability surpassing those cultured in static conditions. Additionally, immunostaining analysis confirmed higher percentage of ki67 positive cells in rotary bioreactor compared with the static culture. In contrast, comparing with static culture, cells in the rotary bioreactor displayed a low expression of involucrin at day 10. Histological analysis revealed that cells cultured in rotary bioreactor aggregated on the micro-carriers and formed multilayer 3D epidermis structures. In conclusion, our research suggests that NASA-approved rotary bioreactor can support the proliferation of hEpSCs and provide a strategy to form multilayer epidermis structure.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22096490</pmid><doi>10.1371/journal.pone.0026603</doi><tpages>e26603</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Biology Biomedical materials Bioreactors Bones Carriers Cell culture Cell Culture Techniques Cell cycle Cell Differentiation - physiology Cell growth Cell Proliferation Children Colonies Differentiation Disease susceptibility Efficiency Epidermis Epidermis - cytology Epidermis - metabolism FDA approval Fluorescent Antibody Technique Humans Integrin beta1 - metabolism Laboratories Lei, Li Medicine Microgravity R&D Research & development Skin Stem cells Stem Cells - cytology Stem Cells - metabolism Studies Tissue engineering Viability Wound healing Zoology |
| title | NASA-approved rotary bioreactor enhances proliferation of human epidermal stem cells and supports formation of 3D epidermis-like structure |
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