Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans
Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr...
Gespeichert in:
| Veröffentlicht in: | PloS one 2011-12, Vol.6 (12), p.e28151-e28151 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e28151 |
|---|---|
| container_issue | 12 |
| container_start_page | e28151 |
| container_title | PloS one |
| container_volume | 6 |
| creator | Ding, Chen Vidanes, Genevieve M Maguire, Sarah L Guida, Alessandro Synnott, John M Andes, David R Butler, Geraldine |
| description | Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and is induced in low iron conditions. All three are involved in the acquisition of iron from heme. However, deletion of the three CFEM genes has no effect on biofilm formation in C. parapsilosis. Our data suggest that the role of the CFEM family in iron acquisition is conserved between C. albicans and C. parapsilosis, but their role in biofilm formation is not. |
| doi_str_mv | 10.1371/journal.pone.0028151 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1311506453</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A476861313</galeid><doaj_id>oai_doaj_org_article_3f2e593a1db246a281b806796846c953</doaj_id><sourcerecordid>A476861313</sourcerecordid><originalsourceid>FETCH-LOGICAL-c691t-757d88d576ec3617dc769de16ea75a2bf91bb81dd8b2c7d160dd56a88473b5b3</originalsourceid><addsrcrecordid>eNqNk11v0zAUhiMEYmPwDxBEQgJx0eKP-CM3SKPaoNLQJJi4tRzbaT25dmYnFfx73DabGrQL5ItY5zzntfP6nKJ4DcEcYgY_3YYheunmXfBmDgDikMAnxSmsMZpRBPDTo_1J8SKlWwAI5pQ-L04QghUBiJ0WehF8MnFrdCm9LrXdmrgyvi9jcCaVoS2_qAj3ucXlxfeyi6E31qfS-nKRo1bLspNRdsm6kGw6kGNCusYq6dPL4lkrXTKvxu9ZcXN5cbP4Nru6_rpcnF_NFK1hP2OEac41YdQoTCHTitFaG0iNZESipq1h03CoNW-QYhpSoDWhkvOK4YY0-Kx4e5Dt8lXE6E8SEENIAK0IzsTyQOggb0UX7UbGPyJIK_aBEFdCxt4qZwRukSE1llA3qKIy29twQFlNeUVVvdf6PJ42NBujVTYtSjcRnWa8XYtV2AqMEGeYZoEPo0AMd4NJvdjYpIxz0pswJFGDGvE6v2Em3_1DPv5zI7WS-f7WtyEfq3aa4rxilNPM7qj5I1Re2mysyr3U2hyfFHycFGSmN7_7lRxSEsufP_6fvf41Zd8fsWsjXb9OwQ29zR05BasDqGJIKZr2wWMIxG4U7t0Qu1EQ4yjksjfH7_NQdN_7-C9zuAIb</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1311506453</pqid></control><display><type>article</type><title>Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Ding, Chen ; Vidanes, Genevieve M ; Maguire, Sarah L ; Guida, Alessandro ; Synnott, John M ; Andes, David R ; Butler, Geraldine</creator><contributor>Arkowitz, Robert Alan</contributor><creatorcontrib>Ding, Chen ; Vidanes, Genevieve M ; Maguire, Sarah L ; Guida, Alessandro ; Synnott, John M ; Andes, David R ; Butler, Geraldine ; Arkowitz, Robert Alan</creatorcontrib><description>Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and is induced in low iron conditions. All three are involved in the acquisition of iron from heme. However, deletion of the three CFEM genes has no effect on biofilm formation in C. parapsilosis. Our data suggest that the role of the CFEM family in iron acquisition is conserved between C. albicans and C. parapsilosis, but their role in biofilm formation is not.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0028151</identifier><identifier>PMID: 22145027</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Biofilms ; Biofilms - growth & development ; Biology ; Biomarkers - metabolism ; Candida ; Candida - metabolism ; Candida - pathogenicity ; Candida albicans ; Candida parapsilosis ; Candidiasis ; Candidiasis - genetics ; Candidiasis - microbiology ; Candidiasis - pathology ; Cell walls ; Clonal deletion ; Cross infection ; Evolution ; Extracellular matrix ; Fungal Proteins - antagonists & inhibitors ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungi ; Gene expression ; Gene Expression Profiling ; Genes ; Genomes ; Genomics ; Health aspects ; Heme ; Hypoxia ; Iron ; Iron - metabolism ; Medical devices ; Medical equipment ; Medical instruments ; Metabolism ; Mycoses ; Oligonucleotide Array Sequence Analysis ; Proteins ; Rats ; Real-Time Polymerase Chain Reaction ; RNA, Messenger - genetics ; Science ; Signal Transduction ; Species Specificity ; Transcription factors ; Virulence ; Yeast</subject><ispartof>PloS one, 2011-12, Vol.6 (12), p.e28151-e28151</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Ding et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Ding et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-757d88d576ec3617dc769de16ea75a2bf91bb81dd8b2c7d160dd56a88473b5b3</citedby><cites>FETCH-LOGICAL-c691t-757d88d576ec3617dc769de16ea75a2bf91bb81dd8b2c7d160dd56a88473b5b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228736/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3228736/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22145027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Arkowitz, Robert Alan</contributor><creatorcontrib>Ding, Chen</creatorcontrib><creatorcontrib>Vidanes, Genevieve M</creatorcontrib><creatorcontrib>Maguire, Sarah L</creatorcontrib><creatorcontrib>Guida, Alessandro</creatorcontrib><creatorcontrib>Synnott, John M</creatorcontrib><creatorcontrib>Andes, David R</creatorcontrib><creatorcontrib>Butler, Geraldine</creatorcontrib><title>Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and is induced in low iron conditions. All three are involved in the acquisition of iron from heme. However, deletion of the three CFEM genes has no effect on biofilm formation in C. parapsilosis. Our data suggest that the role of the CFEM family in iron acquisition is conserved between C. albicans and C. parapsilosis, but their role in biofilm formation is not.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Biology</subject><subject>Biomarkers - metabolism</subject><subject>Candida</subject><subject>Candida - metabolism</subject><subject>Candida - pathogenicity</subject><subject>Candida albicans</subject><subject>Candida parapsilosis</subject><subject>Candidiasis</subject><subject>Candidiasis - genetics</subject><subject>Candidiasis - microbiology</subject><subject>Candidiasis - pathology</subject><subject>Cell walls</subject><subject>Clonal deletion</subject><subject>Cross infection</subject><subject>Evolution</subject><subject>Extracellular matrix</subject><subject>Fungal Proteins - antagonists & inhibitors</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungi</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Heme</subject><subject>Hypoxia</subject><subject>Iron</subject><subject>Iron - metabolism</subject><subject>Medical devices</subject><subject>Medical equipment</subject><subject>Medical instruments</subject><subject>Metabolism</subject><subject>Mycoses</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Proteins</subject><subject>Rats</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Science</subject><subject>Signal Transduction</subject><subject>Species Specificity</subject><subject>Transcription factors</subject><subject>Virulence</subject><subject>Yeast</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQgJx0eKP-CM3SKPaoNLQJJi4tRzbaT25dmYnFfx73DabGrQL5ItY5zzntfP6nKJ4DcEcYgY_3YYheunmXfBmDgDikMAnxSmsMZpRBPDTo_1J8SKlWwAI5pQ-L04QghUBiJ0WehF8MnFrdCm9LrXdmrgyvi9jcCaVoS2_qAj3ucXlxfeyi6E31qfS-nKRo1bLspNRdsm6kGw6kGNCusYq6dPL4lkrXTKvxu9ZcXN5cbP4Nru6_rpcnF_NFK1hP2OEac41YdQoTCHTitFaG0iNZESipq1h03CoNW-QYhpSoDWhkvOK4YY0-Kx4e5Dt8lXE6E8SEENIAK0IzsTyQOggb0UX7UbGPyJIK_aBEFdCxt4qZwRukSE1llA3qKIy29twQFlNeUVVvdf6PJ42NBujVTYtSjcRnWa8XYtV2AqMEGeYZoEPo0AMd4NJvdjYpIxz0pswJFGDGvE6v2Em3_1DPv5zI7WS-f7WtyEfq3aa4rxilNPM7qj5I1Re2mysyr3U2hyfFHycFGSmN7_7lRxSEsufP_6fvf41Zd8fsWsjXb9OwQ29zR05BasDqGJIKZr2wWMIxG4U7t0Qu1EQ4yjksjfH7_NQdN_7-C9zuAIb</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Ding, Chen</creator><creator>Vidanes, Genevieve M</creator><creator>Maguire, Sarah L</creator><creator>Guida, Alessandro</creator><creator>Synnott, John M</creator><creator>Andes, David R</creator><creator>Butler, Geraldine</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20111201</creationdate><title>Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans</title><author>Ding, Chen ; Vidanes, Genevieve M ; Maguire, Sarah L ; Guida, Alessandro ; Synnott, John M ; Andes, David R ; Butler, Geraldine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-757d88d576ec3617dc769de16ea75a2bf91bb81dd8b2c7d160dd56a88473b5b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Biology</topic><topic>Biomarkers - metabolism</topic><topic>Candida</topic><topic>Candida - metabolism</topic><topic>Candida - pathogenicity</topic><topic>Candida albicans</topic><topic>Candida parapsilosis</topic><topic>Candidiasis</topic><topic>Candidiasis - genetics</topic><topic>Candidiasis - microbiology</topic><topic>Candidiasis - pathology</topic><topic>Cell walls</topic><topic>Clonal deletion</topic><topic>Cross infection</topic><topic>Evolution</topic><topic>Extracellular matrix</topic><topic>Fungal Proteins - antagonists & inhibitors</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungi</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Heme</topic><topic>Hypoxia</topic><topic>Iron</topic><topic>Iron - metabolism</topic><topic>Medical devices</topic><topic>Medical equipment</topic><topic>Medical instruments</topic><topic>Metabolism</topic><topic>Mycoses</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Proteins</topic><topic>Rats</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Science</topic><topic>Signal Transduction</topic><topic>Species Specificity</topic><topic>Transcription factors</topic><topic>Virulence</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Chen</creatorcontrib><creatorcontrib>Vidanes, Genevieve M</creatorcontrib><creatorcontrib>Maguire, Sarah L</creatorcontrib><creatorcontrib>Guida, Alessandro</creatorcontrib><creatorcontrib>Synnott, John M</creatorcontrib><creatorcontrib>Andes, David R</creatorcontrib><creatorcontrib>Butler, Geraldine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Chen</au><au>Vidanes, Genevieve M</au><au>Maguire, Sarah L</au><au>Guida, Alessandro</au><au>Synnott, John M</au><au>Andes, David R</au><au>Butler, Geraldine</au><au>Arkowitz, Robert Alan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>6</volume><issue>12</issue><spage>e28151</spage><epage>e28151</epage><pages>e28151-e28151</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Candida parapsilosis is a pathogenic fungus that is major cause of hospital-acquired infection, predominantly due to growth as biofilms on indwelling medical devices. It is related to Candida albicans, which remains the most common cause of candidiasis disease in humans. The transcription factor Bcr1 is an important regulator of biofilm formation in vitro in both C. parapsilosis and C. albicans. We show here that C. parapsilosis Bcr1 is required for in vivo biofilm development in a rat catheter model, like C. albicans. By comparing the transcription profiles of a bcr1 deletion in both species we found that regulation of expression of the CFEM family is conserved. In C. albicans, three of the five CFEM cell wall proteins (Rbt5, Pga7 and Csa1) are associated with both biofilm formation and acquisition of iron from heme, which is an important virulence characteristic. In C. parapsilosis, the CFEM family has undergone an expansion to 7 members. Expression of three genes (CFEM2, CFEM3, and CFEM6) is dependent on Bcr1, and is induced in low iron conditions. All three are involved in the acquisition of iron from heme. However, deletion of the three CFEM genes has no effect on biofilm formation in C. parapsilosis. Our data suggest that the role of the CFEM family in iron acquisition is conserved between C. albicans and C. parapsilosis, but their role in biofilm formation is not.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22145027</pmid><doi>10.1371/journal.pone.0028151</doi><tpages>e28151</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2011-12, Vol.6 (12), p.e28151-e28151 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1311506453 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Biofilms Biofilms - growth & development Biology Biomarkers - metabolism Candida Candida - metabolism Candida - pathogenicity Candida albicans Candida parapsilosis Candidiasis Candidiasis - genetics Candidiasis - microbiology Candidiasis - pathology Cell walls Clonal deletion Cross infection Evolution Extracellular matrix Fungal Proteins - antagonists & inhibitors Fungal Proteins - genetics Fungal Proteins - metabolism Fungi Gene expression Gene Expression Profiling Genes Genomes Genomics Health aspects Heme Hypoxia Iron Iron - metabolism Medical devices Medical equipment Medical instruments Metabolism Mycoses Oligonucleotide Array Sequence Analysis Proteins Rats Real-Time Polymerase Chain Reaction RNA, Messenger - genetics Science Signal Transduction Species Specificity Transcription factors Virulence Yeast |
| title | Conserved and divergent roles of Bcr1 and CFEM proteins in Candida parapsilosis and Candida albicans |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T20%3A54%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Conserved%20and%20divergent%20roles%20of%20Bcr1%20and%20CFEM%20proteins%20in%20Candida%20parapsilosis%20and%20Candida%20albicans&rft.jtitle=PloS%20one&rft.au=Ding,%20Chen&rft.date=2011-12-01&rft.volume=6&rft.issue=12&rft.spage=e28151&rft.epage=e28151&rft.pages=e28151-e28151&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0028151&rft_dat=%3Cgale_plos_%3EA476861313%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1311506453&rft_id=info:pmid/22145027&rft_galeid=A476861313&rft_doaj_id=oai_doaj_org_article_3f2e593a1db246a281b806796846c953&rfr_iscdi=true |