Characterization of fluorescent eye markers for mammalian transgenic studies
Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified...
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| Veröffentlicht in: | PloS one 2011-12, Vol.6 (12), p.e29486-e29486 |
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| creator | Cornett, Jonathan C Landrette, Sean F Xu, Tian |
| description | Genotyping mice by DNA based methods is both laborious and costly. As an alternative, we systematically examined fluorescent proteins expressed in the lens as transgenic markers for mice. A set of eye markers has been selected such that double and triple transgenic animals can be visually identified and that fluorescence intensity in the eyes can be used to distinguish heterozygous from homozygous mice. Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction. |
| doi_str_mv | 10.1371/journal.pone.0029486 |
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Taken together, these eye markers dramatically reduce the time and cost of genotyping transgenics and empower analysis of genetic interaction.</description><subject>Animal genetic engineering</subject><subject>Animals</subject><subject>Biology</subject><subject>Biomarkers - metabolism</subject><subject>Cataracts</subject><subject>Cost analysis</subject><subject>Deoxyribonucleic acid</subject><subject>Discosoma</subject><subject>DNA</subject><subject>Eye</subject><subject>Eye - metabolism</subject><subject>Eye lens</subject><subject>Flashlights</subject><subject>Fluorescence</subject><subject>Genetically modified animals</subject><subject>Genetics</subject><subject>Genotyping</subject><subject>Keratin</subject><subject>Luminescent Proteins - metabolism</subject><subject>Mammals</subject><subject>Markers</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Mutagenesis</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Stem cells</subject><subject>Transgenic animals</subject><subject>Transgenic mice</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLguLFjPlq0twIy-DHwMCCX7chTU9nMqbNmLTi-utNne4ylb2QXCQkz3lP8ubNsqcYLTEV-M3eD6HTbnnwHSwRIpKV_F52jiUlC04QvX-yPssexbhHqKAl5w-zM0II5kSS82yz2umgTQ_B_ta99V3um7xxgw8QDXR9DteQtzp8hxDzxoe0blvtrO7yPugubqGzJo_9UFuIj7MHjXYRnkzzRfb1_bsvq4-LzdWH9epyszCiEP0CmDayrmuKTYk4GFY2SAgoG0MEF6hiuqowAlQ0pKorjqqyZAWThFU154YTepE9P-oenI9qMiIqTDFGJSVEJmJ9JGqv9-oQbHrCtfLaqr8bPmyVDr01DhTTVAqQpJBCMlkRiUxRI4EENhK0Rknr7dRtqFqoR1eCdjPR-Ulnd2rrfypKmCDleN1Xk0DwPwaIvWptMtc53YEfopKYUYQEH1u9-Ie8-3ETtdXp_rZrfGprRk11yQQvixJJmqjlHVQaNbTWpNA0Nu3PCl7PChLTw69-q4cY1frzp_9nr77N2Zcn7A6063fRu2FMW5yD7Aia4GMM0Nx6jJEaM3_jhhozr6bMp7Jnp_9zW3QTcvoHP5z8BA</recordid><startdate>20111228</startdate><enddate>20111228</enddate><creator>Cornett, Jonathan C</creator><creator>Landrette, Sean F</creator><creator>Xu, Tian</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20111228</creationdate><title>Characterization of fluorescent eye markers for mammalian transgenic studies</title><author>Cornett, Jonathan C ; Landrette, Sean F ; Xu, Tian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c757t-e4ac9ddd31c806ec48f077e8fc27670b4abb10e05f2bdb60b88454924bd66c623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animal genetic engineering</topic><topic>Animals</topic><topic>Biology</topic><topic>Biomarkers - 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| subjects | Animal genetic engineering Animals Biology Biomarkers - metabolism Cataracts Cost analysis Deoxyribonucleic acid Discosoma DNA Eye Eye - metabolism Eye lens Flashlights Fluorescence Genetically modified animals Genetics Genotyping Keratin Luminescent Proteins - metabolism Mammals Markers Medicine Mice Mice, Transgenic Mutagenesis Proteins Rodents Stem cells Transgenic animals Transgenic mice |
| title | Characterization of fluorescent eye markers for mammalian transgenic studies |
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