Characterization of a novel type of HIV-1 particle assembly inhibitor using a quantitative luciferase-Vpr packaging-based assay

The HIV-1 auxiliary protein Vpr and Vpr-fusion proteins can be copackaged with Gag precursor (Pr55Gag) into virions or membrane-enveloped virus-like particles (VLP). Taking advantage of this property, we developed a simple and sensitive method to evaluate potential inhibitors of HIV-1 assembly in a...

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Veröffentlicht in:	PloS one 2011-11, Vol.6 (11), p.e27234
Hauptverfasser:	Gonzalez, Gaëlle, DaFonseca, Sandrina, Errazuriz, Elisabeth, Coric, Pascale, Souquet, Florence, Turcaud, Serge, Boulanger, Pierre, Bouaziz, Serge, Hong, Saw See
Format:	Artikel
Sprache:	eng
Schlagworte:	Aberration Alanine Animals Antiviral activity Antiviral agents Assaying Assembly Baculovirus Betulinic acid Biochemistry, Molecular Biology Biological effects Biology Budding Cell culture Cell growth Cell Line Crosslinking Cytochrome Cytotoxicity Derivatives Electrophoresis, Polyacrylamide Gel Enzymatic activity Fusion protein Gag protein Glycine HIV HIV-1 HIV-1 - physiology Human immunodeficiency virus Immunology Intermediates Life Sciences Luciferase Lysine Materials Science Medicine Melanoma Microscopy, Electron Morphology Mutation N-Terminus Nanoparticles Packaging Particulates Pathology Proteins Saccharomyces cerevisiae Spodoptera Structural Biology Toxicity Trimers Virion Virion - physiology Virion - ultrastructure Virions Virus-like particles Viruses vpr Gene Products, Human Immunodeficiency Virus vpr Gene Products, Human Immunodeficiency Virus - metabolism Vpr protein
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