Specific egg yolk immunoglobulin as a new preventive approach for Shiga-toxin-mediated diseases
Shiga toxins (Stxs) are involved in the development of severe systemic complications associated with enterohemorrhagic Escherichia coli (EHEC) infection. Various neutralizing agents against Stxs are under investigation for management of EHEC infection. In this study, we immunized chickens with forma...
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| creator | Neri, Paola Tokoro, Shunji Kobayashi, Ryo Sugiyama, Tsuyoshi Umeda, Kouji Shimizu, Takeshi Tsuji, Takao Kodama, Yoshikatsu Oguma, Keiji Mori, Hiroshi |
| description | Shiga toxins (Stxs) are involved in the development of severe systemic complications associated with enterohemorrhagic Escherichia coli (EHEC) infection. Various neutralizing agents against Stxs are under investigation for management of EHEC infection. In this study, we immunized chickens with formalin-inactivated Stx-1 or Stx-2, and obtained immunoglobulin Y (IgY) from the egg yolk. Anti-Stx-1 IgY and anti-Stx-2 IgY recognized the corresponding Stx A subunit and polymeric but not monomeric B subunit. Anti-Stx-1 IgY and anti-Stx-2 IgY suppressed the cytotoxicity of Stx-1 and Stx-2 to HeLa 229 cells, without cross-suppressive activity. The suppressive activity of these IgY was abrogated by pre-incubation with the corresponding recombinant B subunit, which suggests that the antibodies directed to the polymeric B subunits were predominantly involved in the suppression. In vivo, the intraperitoneal or intravenous administration of these IgY rescued mice from death caused by intraperitoneal injection of the corresponding toxin at a lethal dose. Moreover, oral administration of anti-Stx-2 IgY reduced the mortality of mice infected intestinally with EHEC O157:H7. Our results therefore suggest that anti-Stx IgY antibodies may be considered as preventive agents for Stx-mediated diseases in EHEC infection. |
| doi_str_mv | 10.1371/journal.pone.0026526 |
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Various neutralizing agents against Stxs are under investigation for management of EHEC infection. In this study, we immunized chickens with formalin-inactivated Stx-1 or Stx-2, and obtained immunoglobulin Y (IgY) from the egg yolk. Anti-Stx-1 IgY and anti-Stx-2 IgY recognized the corresponding Stx A subunit and polymeric but not monomeric B subunit. Anti-Stx-1 IgY and anti-Stx-2 IgY suppressed the cytotoxicity of Stx-1 and Stx-2 to HeLa 229 cells, without cross-suppressive activity. The suppressive activity of these IgY was abrogated by pre-incubation with the corresponding recombinant B subunit, which suggests that the antibodies directed to the polymeric B subunits were predominantly involved in the suppression. In vivo, the intraperitoneal or intravenous administration of these IgY rescued mice from death caused by intraperitoneal injection of the corresponding toxin at a lethal dose. Moreover, oral administration of anti-Stx-2 IgY reduced the mortality of mice infected intestinally with EHEC O157:H7. Our results therefore suggest that anti-Stx IgY antibodies may be considered as preventive agents for Stx-mediated diseases in EHEC infection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0026526</identifier><identifier>PMID: 22028896</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Administration, Oral ; Animals ; Antibodies ; Antibody Specificity ; Biocompatibility ; Biology ; Chickens ; Complications ; Cross Reactions - immunology ; Cytotoxicity ; E coli ; Egg Proteins - administration & dosage ; Egg Proteins - immunology ; Enterohemorrhagic Escherichia coli - immunology ; Enterohemorrhagic Escherichia coli - pathogenicity ; Escherichia coli ; Escherichia coli Infections - prevention & control ; Feces ; Female ; Health aspects ; Humans ; Immunization ; Immunization - methods ; Immunoglobulin Y ; Immunoglobulins ; Immunoglobulins - administration & dosage ; Immunoglobulins - immunology ; Incubation ; Infections ; Intravenous administration ; Lethal dose ; Male ; Medicine ; Mice ; Neutralization Tests ; Oral administration ; Pharmaceuticals ; Poultry ; Shiga toxin ; Shiga Toxin 1 - immunology ; Shiga Toxin 2 - immunology ; Species Specificity ; Toxicity ; Toxins ; Yolk</subject><ispartof>PloS one, 2011-10, Vol.6 (10), p.e26526-e26526</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Neri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Neri et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c757t-f0e0220773aa65b7fbb077fdbf082fae119edb242058ad62243362b884cfaf133</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197529/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3197529/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22028896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Neyrolles, Olivier</contributor><creatorcontrib>Neri, Paola</creatorcontrib><creatorcontrib>Tokoro, Shunji</creatorcontrib><creatorcontrib>Kobayashi, Ryo</creatorcontrib><creatorcontrib>Sugiyama, Tsuyoshi</creatorcontrib><creatorcontrib>Umeda, Kouji</creatorcontrib><creatorcontrib>Shimizu, Takeshi</creatorcontrib><creatorcontrib>Tsuji, Takao</creatorcontrib><creatorcontrib>Kodama, Yoshikatsu</creatorcontrib><creatorcontrib>Oguma, Keiji</creatorcontrib><creatorcontrib>Mori, Hiroshi</creatorcontrib><title>Specific egg yolk immunoglobulin as a new preventive approach for Shiga-toxin-mediated diseases</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Shiga toxins (Stxs) are involved in the development of severe systemic complications associated with enterohemorrhagic Escherichia coli (EHEC) infection. Various neutralizing agents against Stxs are under investigation for management of EHEC infection. In this study, we immunized chickens with formalin-inactivated Stx-1 or Stx-2, and obtained immunoglobulin Y (IgY) from the egg yolk. Anti-Stx-1 IgY and anti-Stx-2 IgY recognized the corresponding Stx A subunit and polymeric but not monomeric B subunit. Anti-Stx-1 IgY and anti-Stx-2 IgY suppressed the cytotoxicity of Stx-1 and Stx-2 to HeLa 229 cells, without cross-suppressive activity. The suppressive activity of these IgY was abrogated by pre-incubation with the corresponding recombinant B subunit, which suggests that the antibodies directed to the polymeric B subunits were predominantly involved in the suppression. In vivo, the intraperitoneal or intravenous administration of these IgY rescued mice from death caused by intraperitoneal injection of the corresponding toxin at a lethal dose. Moreover, oral administration of anti-Stx-2 IgY reduced the mortality of mice infected intestinally with EHEC O157:H7. Our results therefore suggest that anti-Stx IgY antibodies may be considered as preventive agents for Stx-mediated diseases in EHEC infection.</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibody Specificity</subject><subject>Biocompatibility</subject><subject>Biology</subject><subject>Chickens</subject><subject>Complications</subject><subject>Cross Reactions - immunology</subject><subject>Cytotoxicity</subject><subject>E coli</subject><subject>Egg Proteins - administration & dosage</subject><subject>Egg Proteins - immunology</subject><subject>Enterohemorrhagic Escherichia coli - immunology</subject><subject>Enterohemorrhagic Escherichia coli - pathogenicity</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - prevention & control</subject><subject>Feces</subject><subject>Female</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Immunization</subject><subject>Immunization - 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Various neutralizing agents against Stxs are under investigation for management of EHEC infection. In this study, we immunized chickens with formalin-inactivated Stx-1 or Stx-2, and obtained immunoglobulin Y (IgY) from the egg yolk. Anti-Stx-1 IgY and anti-Stx-2 IgY recognized the corresponding Stx A subunit and polymeric but not monomeric B subunit. Anti-Stx-1 IgY and anti-Stx-2 IgY suppressed the cytotoxicity of Stx-1 and Stx-2 to HeLa 229 cells, without cross-suppressive activity. The suppressive activity of these IgY was abrogated by pre-incubation with the corresponding recombinant B subunit, which suggests that the antibodies directed to the polymeric B subunits were predominantly involved in the suppression. In vivo, the intraperitoneal or intravenous administration of these IgY rescued mice from death caused by intraperitoneal injection of the corresponding toxin at a lethal dose. Moreover, oral administration of anti-Stx-2 IgY reduced the mortality of mice infected intestinally with EHEC O157:H7. Our results therefore suggest that anti-Stx IgY antibodies may be considered as preventive agents for Stx-mediated diseases in EHEC infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22028896</pmid><doi>10.1371/journal.pone.0026526</doi><tpages>e26526</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Administration, Oral Animals Antibodies Antibody Specificity Biocompatibility Biology Chickens Complications Cross Reactions - immunology Cytotoxicity E coli Egg Proteins - administration & dosage Egg Proteins - immunology Enterohemorrhagic Escherichia coli - immunology Enterohemorrhagic Escherichia coli - pathogenicity Escherichia coli Escherichia coli Infections - prevention & control Feces Female Health aspects Humans Immunization Immunization - methods Immunoglobulin Y Immunoglobulins Immunoglobulins - administration & dosage Immunoglobulins - immunology Incubation Infections Intravenous administration Lethal dose Male Medicine Mice Neutralization Tests Oral administration Pharmaceuticals Poultry Shiga toxin Shiga Toxin 1 - immunology Shiga Toxin 2 - immunology Species Specificity Toxicity Toxins Yolk |
| title | Specific egg yolk immunoglobulin as a new preventive approach for Shiga-toxin-mediated diseases |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T10%3A06%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Specific%20egg%20yolk%20immunoglobulin%20as%20a%20new%20preventive%20approach%20for%20Shiga-toxin-mediated%20diseases&rft.jtitle=PloS%20one&rft.au=Neri,%20Paola&rft.date=2011-10-19&rft.volume=6&rft.issue=10&rft.spage=e26526&rft.epage=e26526&rft.pages=e26526-e26526&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0026526&rft_dat=%3Cgale_plos_%3EA476867507%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1310270440&rft_id=info:pmid/22028896&rft_galeid=A476867507&rft_doaj_id=oai_doaj_org_article_0749d047d67f4ec4aab254b0f9fc0705&rfr_iscdi=true |