OmniChange: the sequence independent method for simultaneous site-saturation of five codons

Focused mutant library generation methods have been developed to improve mainly "localizable" enzyme properties such as activity and selectivity. Current multi-site saturation methods are restricted by the gene sequence, require subsequent PCR steps and/or additional enzymatic modification...

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Veröffentlicht in:	PloS one 2011-10, Vol.6 (10), p.e26222
Hauptverfasser:	Dennig, Alexander, Shivange, Amol V, Marienhagen, Jan, Schwaneberg, Ulrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Analysis Base Sequence Biocatalysts Biology Chemistry Cloning Codon Codons Degeneration Deoxyribonucleic acid DNA E coli Efficiency Enzymes Escherichia coli Genomes Hybridization Laboratories Ligases Methods Mutagenesis Plasmids Polymerase Chain Reaction Proteins Saturation Saturation mutagenesis Selectivity Synthetic biology
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