The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH

The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture p...

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Veröffentlicht in:PloS one 2011-10, Vol.6 (10), p.e26695
Hauptverfasser: Crawfurd, Katharine J, Raven, John A, Wheeler, Glen L, Baxter, Emily J, Joint, Ian
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Sprache:eng
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Zusammenfassung:The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture pH changed rapidly because of CO(2) (aq) assimilation and pH targets of 7.8 and 8.1 could not be sustained. Long-term (∼100 generation) pH-auxostat, continuous cultures could be maintained at target pH when cell density was kept low (
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0026695