TLR7 and TLR8 gene variations and susceptibility to hepatitis C virus infection
Toll-like receptors (TLRs) play pivotal roles in the innate immune system and control inflammatory responses and adaptive immunity. We previously evaluated associations between TLR7 and TLR8 gene SNPs and susceptibility to hepatitis C virus (HCV) infection. Our results suggested that TLR7IVS2-151G a...
Gespeichert in:
| Veröffentlicht in: | PloS one 2011-10, Vol.6 (10), p.e26235-e26235 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e26235 |
|---|---|
| container_issue | 10 |
| container_start_page | e26235 |
| container_title | PloS one |
| container_volume | 6 |
| creator | Wang, Chiou-Huey Eng, Hock-Liew Lin, Kuei-Hsiang Chang, Cheng-Hsien Hsieh, Chi-An Lin, Yen-Li Lin, Tsun-Mei |
| description | Toll-like receptors (TLRs) play pivotal roles in the innate immune system and control inflammatory responses and adaptive immunity. We previously evaluated associations between TLR7 and TLR8 gene SNPs and susceptibility to hepatitis C virus (HCV) infection. Our results suggested that TLR7IVS2-151G and TLR8-129G alleles were present at higher frequency in males of an HCV-infected group as compared to a control group (24.1% vs. 14.4%, p = 0.028; 17.6% vs. 6.8%, p = 0.004, respectively). Based upon their recognition of single stranded viral RNA, this suggested that TLR7 and TLR8 played a significant role in anti-HCV immune responses. Here, we studied the functional effects of these polymorphisms by analyzing the mRNA expressions of TLR7 and TLR8 and cytokine production induced ex vivo by TLR7- and TLR8-specific agonists using whole blood of subjects with different genotypes. The percentage of CD14+ cells from those with an AG haplotype that expressed TLR7 and TLR8 was significantly lower, but higher in intensity compared to cells from those with GG and AC haplotypes. Cells from those with an AG haplotype produced more IFN-α and less amounts of pro-inflammatory cytokines upon stimulation. This suggests that variations in TLR7 and TLR8 genes might impair immune responses during HCV infection. |
| doi_str_mv | 10.1371/journal.pone.0026235 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1309830436</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A476868688</galeid><doaj_id>oai_doaj_org_article_9c50e30166044796bc00f9e64ea1b7f3</doaj_id><sourcerecordid>A476868688</sourcerecordid><originalsourceid>FETCH-LOGICAL-c691t-b9b4db71c33ad258013f97d1b6b0573402a916772aba4b865a6645cca2659baf3</originalsourceid><addsrcrecordid>eNqNkl-LEzEUxQdR3LX6DUQHBMWH1ptkJpm8CEvxT6FQWFdfQ5LJtFmmk5pkivvtTdvZpSP7IHlIyP3dk-TkZNlrBDNEGPp063rfyXa2c52ZAWCKSfkku0Sc4CnFQJ6erS-yFyHcApSkovR5doExYFwyepmtbpbXLJddnadFla9NZ_K99FZG67pwLIQ-aLOLVtnWxrs8unxjdqkebcjn-d76PuS2a4w-tLzMnjWyDebVME-yn1-_3My_T5erb4v51XKqKUdxqrgqasWQJkTWuKwAkYazGimqoGSkACw5ooxhqWShKlpKSotSa4lpyZVsyCR7e9LdtS6IwYsgEAFeESgITcTiRNRO3oqdt1vp74STVhw3nF8L6aPVrRFcl2AIIEqhKBinSgM03NDCSKRYQ5LW5-G0Xm1NrU0XvWxHouNKZzdi7faCII4ZhyTwYRDw7ndvQhRbm1xtW9kZ1wfBAWgBND19kr37h3z8cQO1lun-yX2XjtUHTXFVMFodRpWo2SNUGrXZWp1y09i0P2r4OGpITDR_4lr2IYjFj-v_Z1e_xuz7M3ZjZBs3wbX9MWRjsDiB2rsQvGkePEYgDrG_d0McYi-G2Ke2N-f_89B0n3PyF99K-zo</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1309830436</pqid></control><display><type>article</type><title>TLR7 and TLR8 gene variations and susceptibility to hepatitis C virus infection</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Wang, Chiou-Huey ; Eng, Hock-Liew ; Lin, Kuei-Hsiang ; Chang, Cheng-Hsien ; Hsieh, Chi-An ; Lin, Yen-Li ; Lin, Tsun-Mei</creator><contributor>Sambhara, Suryaprakash</contributor><creatorcontrib>Wang, Chiou-Huey ; Eng, Hock-Liew ; Lin, Kuei-Hsiang ; Chang, Cheng-Hsien ; Hsieh, Chi-An ; Lin, Yen-Li ; Lin, Tsun-Mei ; Sambhara, Suryaprakash</creatorcontrib><description>Toll-like receptors (TLRs) play pivotal roles in the innate immune system and control inflammatory responses and adaptive immunity. We previously evaluated associations between TLR7 and TLR8 gene SNPs and susceptibility to hepatitis C virus (HCV) infection. Our results suggested that TLR7IVS2-151G and TLR8-129G alleles were present at higher frequency in males of an HCV-infected group as compared to a control group (24.1% vs. 14.4%, p = 0.028; 17.6% vs. 6.8%, p = 0.004, respectively). Based upon their recognition of single stranded viral RNA, this suggested that TLR7 and TLR8 played a significant role in anti-HCV immune responses. Here, we studied the functional effects of these polymorphisms by analyzing the mRNA expressions of TLR7 and TLR8 and cytokine production induced ex vivo by TLR7- and TLR8-specific agonists using whole blood of subjects with different genotypes. The percentage of CD14+ cells from those with an AG haplotype that expressed TLR7 and TLR8 was significantly lower, but higher in intensity compared to cells from those with GG and AC haplotypes. Cells from those with an AG haplotype produced more IFN-α and less amounts of pro-inflammatory cytokines upon stimulation. This suggests that variations in TLR7 and TLR8 genes might impair immune responses during HCV infection.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0026235</identifier><identifier>PMID: 22022576</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptive control ; Adaptive immunity ; Antigens ; B cells ; Biology ; Case-Control Studies ; CD14 antigen ; Chlamydia ; Chromosomes ; Control systems ; Cytokines ; Cytokines - biosynthesis ; Dendritic cells ; Development and progression ; Disease susceptibility ; Ethnic Groups - genetics ; Female ; Females ; Gene expression ; Gene Expression Regulation ; Gene Frequency - genetics ; Genes ; Genetic aspects ; Genetic Predisposition to Disease ; Genotypes ; Haplotypes ; Haplotypes - genetics ; Health aspects ; Hepacivirus - physiology ; Hepatitis ; Hepatitis C ; Hepatitis C virus ; Hepatitis C, Chronic - genetics ; Hospitals ; Humans ; Immune response ; Immune system ; Immunity ; Infection ; Infections ; Inflammation ; Innate immunity ; Interferon ; Intracellular Space - metabolism ; Male ; Males ; Medical laboratories ; Medicine ; Middle Aged ; mRNA ; Polymorphism, Single Nucleotide - genetics ; Proteins ; Receptors ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Single nucleotide polymorphisms ; Single-nucleotide polymorphism ; Studies ; TLR7 protein ; Toll-Like Receptor 7 - agonists ; Toll-Like Receptor 7 - genetics ; Toll-Like Receptor 7 - metabolism ; Toll-Like Receptor 8 - agonists ; Toll-Like Receptor 8 - genetics ; Toll-Like Receptor 8 - metabolism ; Toll-like receptors ; Viruses</subject><ispartof>PloS one, 2011-10, Vol.6 (10), p.e26235-e26235</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Wang et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-b9b4db71c33ad258013f97d1b6b0573402a916772aba4b865a6645cca2659baf3</citedby><cites>FETCH-LOGICAL-c691t-b9b4db71c33ad258013f97d1b6b0573402a916772aba4b865a6645cca2659baf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192790/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3192790/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22022576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Sambhara, Suryaprakash</contributor><creatorcontrib>Wang, Chiou-Huey</creatorcontrib><creatorcontrib>Eng, Hock-Liew</creatorcontrib><creatorcontrib>Lin, Kuei-Hsiang</creatorcontrib><creatorcontrib>Chang, Cheng-Hsien</creatorcontrib><creatorcontrib>Hsieh, Chi-An</creatorcontrib><creatorcontrib>Lin, Yen-Li</creatorcontrib><creatorcontrib>Lin, Tsun-Mei</creatorcontrib><title>TLR7 and TLR8 gene variations and susceptibility to hepatitis C virus infection</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Toll-like receptors (TLRs) play pivotal roles in the innate immune system and control inflammatory responses and adaptive immunity. We previously evaluated associations between TLR7 and TLR8 gene SNPs and susceptibility to hepatitis C virus (HCV) infection. Our results suggested that TLR7IVS2-151G and TLR8-129G alleles were present at higher frequency in males of an HCV-infected group as compared to a control group (24.1% vs. 14.4%, p = 0.028; 17.6% vs. 6.8%, p = 0.004, respectively). Based upon their recognition of single stranded viral RNA, this suggested that TLR7 and TLR8 played a significant role in anti-HCV immune responses. Here, we studied the functional effects of these polymorphisms by analyzing the mRNA expressions of TLR7 and TLR8 and cytokine production induced ex vivo by TLR7- and TLR8-specific agonists using whole blood of subjects with different genotypes. The percentage of CD14+ cells from those with an AG haplotype that expressed TLR7 and TLR8 was significantly lower, but higher in intensity compared to cells from those with GG and AC haplotypes. Cells from those with an AG haplotype produced more IFN-α and less amounts of pro-inflammatory cytokines upon stimulation. This suggests that variations in TLR7 and TLR8 genes might impair immune responses during HCV infection.</description><subject>Adaptive control</subject><subject>Adaptive immunity</subject><subject>Antigens</subject><subject>B cells</subject><subject>Biology</subject><subject>Case-Control Studies</subject><subject>CD14 antigen</subject><subject>Chlamydia</subject><subject>Chromosomes</subject><subject>Control systems</subject><subject>Cytokines</subject><subject>Cytokines - biosynthesis</subject><subject>Dendritic cells</subject><subject>Development and progression</subject><subject>Disease susceptibility</subject><subject>Ethnic Groups - genetics</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene Frequency - genetics</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotypes</subject><subject>Haplotypes</subject><subject>Haplotypes - genetics</subject><subject>Health aspects</subject><subject>Hepacivirus - physiology</subject><subject>Hepatitis</subject><subject>Hepatitis C</subject><subject>Hepatitis C virus</subject><subject>Hepatitis C, Chronic - genetics</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Infection</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Innate immunity</subject><subject>Interferon</subject><subject>Intracellular Space - metabolism</subject><subject>Male</subject><subject>Males</subject><subject>Medical laboratories</subject><subject>Medicine</subject><subject>Middle Aged</subject><subject>mRNA</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Single nucleotide polymorphisms</subject><subject>Single-nucleotide polymorphism</subject><subject>Studies</subject><subject>TLR7 protein</subject><subject>Toll-Like Receptor 7 - agonists</subject><subject>Toll-Like Receptor 7 - genetics</subject><subject>Toll-Like Receptor 7 - metabolism</subject><subject>Toll-Like Receptor 8 - agonists</subject><subject>Toll-Like Receptor 8 - genetics</subject><subject>Toll-Like Receptor 8 - metabolism</subject><subject>Toll-like receptors</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl-LEzEUxQdR3LX6DUQHBMWH1ptkJpm8CEvxT6FQWFdfQ5LJtFmmk5pkivvtTdvZpSP7IHlIyP3dk-TkZNlrBDNEGPp063rfyXa2c52ZAWCKSfkku0Sc4CnFQJ6erS-yFyHcApSkovR5doExYFwyepmtbpbXLJddnadFla9NZ_K99FZG67pwLIQ-aLOLVtnWxrs8unxjdqkebcjn-d76PuS2a4w-tLzMnjWyDebVME-yn1-_3My_T5erb4v51XKqKUdxqrgqasWQJkTWuKwAkYazGimqoGSkACw5ooxhqWShKlpKSotSa4lpyZVsyCR7e9LdtS6IwYsgEAFeESgITcTiRNRO3oqdt1vp74STVhw3nF8L6aPVrRFcl2AIIEqhKBinSgM03NDCSKRYQ5LW5-G0Xm1NrU0XvWxHouNKZzdi7faCII4ZhyTwYRDw7ndvQhRbm1xtW9kZ1wfBAWgBND19kr37h3z8cQO1lun-yX2XjtUHTXFVMFodRpWo2SNUGrXZWp1y09i0P2r4OGpITDR_4lr2IYjFj-v_Z1e_xuz7M3ZjZBs3wbX9MWRjsDiB2rsQvGkePEYgDrG_d0McYi-G2Ke2N-f_89B0n3PyF99K-zo</recordid><startdate>20111013</startdate><enddate>20111013</enddate><creator>Wang, Chiou-Huey</creator><creator>Eng, Hock-Liew</creator><creator>Lin, Kuei-Hsiang</creator><creator>Chang, Cheng-Hsien</creator><creator>Hsieh, Chi-An</creator><creator>Lin, Yen-Li</creator><creator>Lin, Tsun-Mei</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20111013</creationdate><title>TLR7 and TLR8 gene variations and susceptibility to hepatitis C virus infection</title><author>Wang, Chiou-Huey ; Eng, Hock-Liew ; Lin, Kuei-Hsiang ; Chang, Cheng-Hsien ; Hsieh, Chi-An ; Lin, Yen-Li ; Lin, Tsun-Mei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-b9b4db71c33ad258013f97d1b6b0573402a916772aba4b865a6645cca2659baf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adaptive control</topic><topic>Adaptive immunity</topic><topic>Antigens</topic><topic>B cells</topic><topic>Biology</topic><topic>Case-Control Studies</topic><topic>CD14 antigen</topic><topic>Chlamydia</topic><topic>Chromosomes</topic><topic>Control systems</topic><topic>Cytokines</topic><topic>Cytokines - biosynthesis</topic><topic>Dendritic cells</topic><topic>Development and progression</topic><topic>Disease susceptibility</topic><topic>Ethnic Groups - genetics</topic><topic>Female</topic><topic>Females</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gene Frequency - genetics</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic Predisposition to Disease</topic><topic>Genotypes</topic><topic>Haplotypes</topic><topic>Haplotypes - genetics</topic><topic>Health aspects</topic><topic>Hepacivirus - physiology</topic><topic>Hepatitis</topic><topic>Hepatitis C</topic><topic>Hepatitis C virus</topic><topic>Hepatitis C, Chronic - genetics</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Infection</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Innate immunity</topic><topic>Interferon</topic><topic>Intracellular Space - metabolism</topic><topic>Male</topic><topic>Males</topic><topic>Medical laboratories</topic><topic>Medicine</topic><topic>Middle Aged</topic><topic>mRNA</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Single nucleotide polymorphisms</topic><topic>Single-nucleotide polymorphism</topic><topic>Studies</topic><topic>TLR7 protein</topic><topic>Toll-Like Receptor 7 - agonists</topic><topic>Toll-Like Receptor 7 - genetics</topic><topic>Toll-Like Receptor 7 - metabolism</topic><topic>Toll-Like Receptor 8 - agonists</topic><topic>Toll-Like Receptor 8 - genetics</topic><topic>Toll-Like Receptor 8 - metabolism</topic><topic>Toll-like receptors</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Chiou-Huey</creatorcontrib><creatorcontrib>Eng, Hock-Liew</creatorcontrib><creatorcontrib>Lin, Kuei-Hsiang</creatorcontrib><creatorcontrib>Chang, Cheng-Hsien</creatorcontrib><creatorcontrib>Hsieh, Chi-An</creatorcontrib><creatorcontrib>Lin, Yen-Li</creatorcontrib><creatorcontrib>Lin, Tsun-Mei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Chiou-Huey</au><au>Eng, Hock-Liew</au><au>Lin, Kuei-Hsiang</au><au>Chang, Cheng-Hsien</au><au>Hsieh, Chi-An</au><au>Lin, Yen-Li</au><au>Lin, Tsun-Mei</au><au>Sambhara, Suryaprakash</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TLR7 and TLR8 gene variations and susceptibility to hepatitis C virus infection</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-10-13</date><risdate>2011</risdate><volume>6</volume><issue>10</issue><spage>e26235</spage><epage>e26235</epage><pages>e26235-e26235</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Toll-like receptors (TLRs) play pivotal roles in the innate immune system and control inflammatory responses and adaptive immunity. We previously evaluated associations between TLR7 and TLR8 gene SNPs and susceptibility to hepatitis C virus (HCV) infection. Our results suggested that TLR7IVS2-151G and TLR8-129G alleles were present at higher frequency in males of an HCV-infected group as compared to a control group (24.1% vs. 14.4%, p = 0.028; 17.6% vs. 6.8%, p = 0.004, respectively). Based upon their recognition of single stranded viral RNA, this suggested that TLR7 and TLR8 played a significant role in anti-HCV immune responses. Here, we studied the functional effects of these polymorphisms by analyzing the mRNA expressions of TLR7 and TLR8 and cytokine production induced ex vivo by TLR7- and TLR8-specific agonists using whole blood of subjects with different genotypes. The percentage of CD14+ cells from those with an AG haplotype that expressed TLR7 and TLR8 was significantly lower, but higher in intensity compared to cells from those with GG and AC haplotypes. Cells from those with an AG haplotype produced more IFN-α and less amounts of pro-inflammatory cytokines upon stimulation. This suggests that variations in TLR7 and TLR8 genes might impair immune responses during HCV infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22022576</pmid><doi>10.1371/journal.pone.0026235</doi><tpages>e26235</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2011-10, Vol.6 (10), p.e26235-e26235 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1309830436 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adaptive control Adaptive immunity Antigens B cells Biology Case-Control Studies CD14 antigen Chlamydia Chromosomes Control systems Cytokines Cytokines - biosynthesis Dendritic cells Development and progression Disease susceptibility Ethnic Groups - genetics Female Females Gene expression Gene Expression Regulation Gene Frequency - genetics Genes Genetic aspects Genetic Predisposition to Disease Genotypes Haplotypes Haplotypes - genetics Health aspects Hepacivirus - physiology Hepatitis Hepatitis C Hepatitis C virus Hepatitis C, Chronic - genetics Hospitals Humans Immune response Immune system Immunity Infection Infections Inflammation Innate immunity Interferon Intracellular Space - metabolism Male Males Medical laboratories Medicine Middle Aged mRNA Polymorphism, Single Nucleotide - genetics Proteins Receptors Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism Single nucleotide polymorphisms Single-nucleotide polymorphism Studies TLR7 protein Toll-Like Receptor 7 - agonists Toll-Like Receptor 7 - genetics Toll-Like Receptor 7 - metabolism Toll-Like Receptor 8 - agonists Toll-Like Receptor 8 - genetics Toll-Like Receptor 8 - metabolism Toll-like receptors Viruses |
| title | TLR7 and TLR8 gene variations and susceptibility to hepatitis C virus infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T02%3A09%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=TLR7%20and%20TLR8%20gene%20variations%20and%20susceptibility%20to%20hepatitis%20C%20virus%20infection&rft.jtitle=PloS%20one&rft.au=Wang,%20Chiou-Huey&rft.date=2011-10-13&rft.volume=6&rft.issue=10&rft.spage=e26235&rft.epage=e26235&rft.pages=e26235-e26235&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0026235&rft_dat=%3Cgale_plos_%3EA476868688%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1309830436&rft_id=info:pmid/22022576&rft_galeid=A476868688&rft_doaj_id=oai_doaj_org_article_9c50e30166044796bc00f9e64ea1b7f3&rfr_iscdi=true |