A time-series method for automated measurement of changes in mitotic and interphase duration from time-lapse movies

Automated time-lapse microscopy can visualize proliferation of large numbers of individual cells, enabling accurate measurement of the frequency of cell division and the duration of interphase and mitosis. However, extraction of quantitative information by manual inspection of time-lapse movies is t...

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Veröffentlicht in:	PloS one 2011-09, Vol.6 (9), p.e25511-e25511
Hauptverfasser:	Sigoillot, Frederic D, Huckins, Jeremy F, Li, Fuhai, Zhou, Xiaobo, Wong, Stephen T C, King, Randall W
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Automation Bioengineering Biology Cancer Cell cycle Cell Cycle - genetics Cell Cycle - physiology Cell division Cell Division - genetics Cell Division - physiology Cell growth Cell Line Cell proliferation Chemotherapy Comparative analysis Datasets Deoxyribonucleic acid DNA DNA damage Experiments Fluorescence Free radicals HeLa Cells Histones - genetics Histones - metabolism Humans Inspection Interphase Interphase - genetics Interphase - physiology Measurement Methods Microscopy Microscopy, Fluorescence - methods Mitochondrial DNA Mitosis Mitosis - genetics Mitosis - physiology Movies Open systems Proteins siRNA Technology application Time series Time-Lapse Imaging - methods
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