Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages
The eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of me...
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| description | The eukaryotic cell membrane possesses numerous complex functions, which are essential for life. At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of membrane lipid composition affecting numerous physiological processes, e.g. in the immune system. In this systematic study we present fatty acid and peptide profiles of cell membrane and membrane rafts of murine macrophages that have been supplemented with saturated fatty acids as well as PUFAs from the n-3, the n-6 and the n-9 family. Using fatty acid composition analysis and mass spectrometry-based peptidome profiling we found that PUFAs from both the n-3 and the n-6 family have an impact on lipid and protein composition of plasma membrane and membrane rafts in a similar manner. In addition, we found a relation between the number of bis-allyl-methylene positions of the PUFA added and the unsaturation index of plasma membrane as well as membrane rafts of supplemented cells. With regard to the proposed significance of lipid microdomains for disease development and treatment our study will help to achieve a targeted dietary modulation of immune cell lipid bilayers. |
| doi_str_mv | 10.1371/journal.pone.0024066 |
| format | Article |
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At this, the composition and the structure of the lipid bilayer are of particular importance. Polyunsaturated fatty acids may modulate the physical properties of biological membranes via alteration of membrane lipid composition affecting numerous physiological processes, e.g. in the immune system. In this systematic study we present fatty acid and peptide profiles of cell membrane and membrane rafts of murine macrophages that have been supplemented with saturated fatty acids as well as PUFAs from the n-3, the n-6 and the n-9 family. Using fatty acid composition analysis and mass spectrometry-based peptidome profiling we found that PUFAs from both the n-3 and the n-6 family have an impact on lipid and protein composition of plasma membrane and membrane rafts in a similar manner. In addition, we found a relation between the number of bis-allyl-methylene positions of the PUFA added and the unsaturation index of plasma membrane as well as membrane rafts of supplemented cells. With regard to the proposed significance of lipid microdomains for disease development and treatment our study will help to achieve a targeted dietary modulation of immune cell lipid bilayers.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21887374</pmid><doi>10.1371/journal.pone.0024066</doi><tpages>e24066</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antigen presentation Biological membranes Biological properties Biology Cell Line Cell Membrane - chemistry Cell membranes Cooperation Diet Fatty acid composition Fatty acids Fatty Acids - analysis Fatty Acids - metabolism Fatty Acids, Omega-3 Fatty Acids, Unsaturated - administration & dosage Fatty Acids, Unsaturated - metabolism Gene expression Growth factors Immune system Immune System - cytology Immune System - metabolism Kinases Laboratories Ligands Lipid bilayers Lipid composition Lipid rafts Lipids Macrophages Macrophages - chemistry Macrophages - ultrastructure Mass spectrometry Mass spectroscopy Medical treatment Medicine Membrane lipids Membrane Lipids - analysis Membrane Microdomains - chemistry Membrane proteins Membranes Mice Pancreatic cancer Peptides Physical properties Physiological aspects Physiology Polyunsaturated fatty acids Protein composition Proteins Rafts Saturated fatty acids Unsaturated fatty acids Veterinary medicine |
| title | Fatty acid and peptide profiles in plasma membrane and membrane rafts of PUFA supplemented RAW264.7 macrophages |
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