Formation of AAV single stranded DNA genome from a circular plasmid in Saccharomyces cerevisiae

Adeno-associated virus (AAV)-based vectors are promising tools for targeted transfer in gene therapy studies. Many efforts have been accomplished to improve production and purification methods. We thought to develop a simple eukaryotic system allowing AAV replication which could provide an excellent...

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Veröffentlicht in:	PloS one 2011-08, Vol.6 (8), p.e23474-e23474
Hauptverfasser:	Cervelli, Tiziana, Backovic, Ana, Galli, Alvaro
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analysis Baking yeast Biology Carcinogens Clinical trials Cloning Cytotoxicity Deoxyribonucleic acid Dependovirus - genetics Dependovirus - physiology DNA DNA Replication DNA, Single-Stranded - genetics DNA, Viral - genetics Expression vectors Fungal Proteins - metabolism Gene expression Gene sequencing Gene therapy Genes Genetic aspects Genetic engineering Genetic research Genetic vectors Genome, Viral - genetics Genomes Genomics Hepatitis Low molecular weights Models, Biological Molecular weight Plant Proteins - metabolism Plasmids Plasmids - genetics Production methods Proteins Purification Replication Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - genetics Single-Strand Specific DNA and RNA Endonucleases - metabolism Southern blotting Terminal Repeat Sequences - genetics URA3 gene Vectors (Biology) Vigna radiata Viral Proteins - metabolism Virus Replication - physiology Viruses Yeast
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