Functional analysis of the Phycomyces carRA gene encoding the enzymes phytoene synthase and lycopene cyclase

Phycomyces carRA gene encodes a protein with two domains. Domain R is characterized by red carR mutants that accumulate lycopene. Domain A is characterized by white carA mutants that do not accumulate significant amounts of carotenoids. The carRA-encoded protein was identified as the lycopene cyclas...

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Veröffentlicht in:	PloS one 2011-08, Vol.6 (8), p.e23102-e23102
Hauptverfasser:	Sanz, Catalina, Velayos, Antonio, Álvarez, María Isabel, Benito, Ernesto P, Eslava, Arturo P
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkyl and Aryl Transferases - genetics Alkyl and Aryl Transferases - metabolism Analysis Bacteria beta Carotene - biosynthesis Biological products Biology Biosynthesis Blotting, Northern Carotenoids Carotenoids - biosynthesis Cloning Complementation Dehydrogenases Deoxyribonucleic acid DNA E coli Enzymes Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - metabolism Functional analysis Fungal Proteins - genetics Fungal Proteins - metabolism Fungi Gene expression Gene Expression Regulation, Enzymologic Genetic Complementation Test Genetic transformation Genetics Geranylgeranyl-Diphosphate Geranylgeranyltransferase Homology Intramolecular Lyases - genetics Intramolecular Lyases - metabolism Laboratories Light Lycopene Medicine Molecular weight mRNA Mucor - enzymology Mucor - genetics Mucor - metabolism Mucor circinelloides Mutants Mutation Phycomyces Phycomyces - enzymology Phycomyces - genetics Phycomyces - metabolism Phytoene synthase Polymerase chain reaction Proteins Reverse Transcriptase Polymerase Chain Reaction RNA Transformation Vectors (Biology) β-Carotene
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