Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development
Xvent homeobox genes encode transcription factors that repress organizer genes and are essential for dorsoventral specification during early embryogenesis in Xenopus. In contrast to the Xvent-2 gene subfamily, Xvent-1 subfamily members, including PV.1A, have been proposed as indirect targets of Bone...
Gespeichert in:
| Veröffentlicht in: | PloS one 2011-08, Vol.6 (8), p.e22621 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 8 |
| container_start_page | e22621 |
| container_title | PloS one |
| container_volume | 6 |
| creator | Lee, Hyun-Shik Lee, Sung-Young Lee, Hyosang Hwang, Yoo-Seok Cha, Sang-Wook Park, Soochul Lee, Jae-Yong Park, Jae-Bong Kim, Sungchan Park, Mae Ja Kim, Jaebong |
| description | Xvent homeobox genes encode transcription factors that repress organizer genes and are essential for dorsoventral specification during early embryogenesis in Xenopus. In contrast to the Xvent-2 gene subfamily, Xvent-1 subfamily members, including PV.1A, have been proposed as indirect targets of Bone Morphogenetic Protein-4 (BMP-4) signaling. Because PV.1A is a critical downstream mediator of, and tightly regulated by, BMP-4 signaling, we hypothesized that its promoter contains a direct BMP-4 response element to effect this transcriptional regulation. We demonstrate that direct regulation by BMP-4 is necessary for transcription of PV.1A: its proximal promoter contains cis-acting binding elements for Smads and Oaz crucial to induction in response to BMP-4 signaling. In addition to these direct cis-acting BMP-4 responsive elements, an indirect Xvent-2 response element and several repressive elements exist in the PV.1A promoter to regulate its transcription. In summary, PV.1A undergoes combinatorial regulation during early Xenopus development as both the direct target of BMP-4 signaling and as the direct and indirect target of positive and negative regulatory factors. |
| doi_str_mv | 10.1371/journal.pone.0022621 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1306895783</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A476883337</galeid><doaj_id>oai_doaj_org_article_79101688ad3f4c209e0a148dfa7999f7</doaj_id><sourcerecordid>A476883337</sourcerecordid><originalsourceid>FETCH-LOGICAL-c691t-6aade8f2d21fa023065386491b6c899d1cb422f304000b5d8df0d9d1e69501733</originalsourceid><addsrcrecordid>eNqNk9tu1DAQhiMEoqXwBggsIYG42MWOc7BvKi3ltFJRKw4Vd5Y3nux65cTBdgp9Bl4ap5tWG9QLlIskk2_-f2biSZKnBM8JLcmbre1dK828sy3MMU7TIiX3kkPCaTorUkzv7z0fJI-832KcU1YUD5ODlLC85JQdJn_eaQdVQA58FPKAwEADbfDI1ujt53P0S4eNblHYADq_mJMF6pxtbACHpIu09xHW0qDaOqRjWnCy9ZXTXdA2lheF172RwwtSvdPtGoF05gr9gNZ2vUcKLsHYbvB8nDyopfHwZLwfJd8_vP928ml2evZxebI4nVUFJ2FWSKmA1alKSS1xSnExtJVxsioqxrki1SpL05riDGO8yhVTNVYxDAXPMSkpPUqe73Q7Y70Y5-gFiUqM5yUbiOWOUFZuRed0I92VsFKL64B1ayFd0JUBUXKCScGYVLTOqhRzwJJk0VOWnPO6jFrHo1u_akBVsVEnzUR0-qXVG7G2l4KSnHI6CLwaBZz92YMPotG-AmNkC7b3grGMZQUuhrJf_EPe3dxIrWWsX7e1jbbVoCkWWRlbofTadX4HFS8Fja7imat1jE8SXk8SIhPgd1jL3nux_Prl_9mziyn7co_dgDRh463phyPlp2C2AytnvXdQ386YYDGszM00xLAyYlyZmPZs___cJt3sCP0Lzc4Scw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1306895783</pqid></control><display><type>article</type><title>Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Lee, Hyun-Shik ; Lee, Sung-Young ; Lee, Hyosang ; Hwang, Yoo-Seok ; Cha, Sang-Wook ; Park, Soochul ; Lee, Jae-Yong ; Park, Jae-Bong ; Kim, Sungchan ; Park, Mae Ja ; Kim, Jaebong</creator><creatorcontrib>Lee, Hyun-Shik ; Lee, Sung-Young ; Lee, Hyosang ; Hwang, Yoo-Seok ; Cha, Sang-Wook ; Park, Soochul ; Lee, Jae-Yong ; Park, Jae-Bong ; Kim, Sungchan ; Park, Mae Ja ; Kim, Jaebong</creatorcontrib><description>Xvent homeobox genes encode transcription factors that repress organizer genes and are essential for dorsoventral specification during early embryogenesis in Xenopus. In contrast to the Xvent-2 gene subfamily, Xvent-1 subfamily members, including PV.1A, have been proposed as indirect targets of Bone Morphogenetic Protein-4 (BMP-4) signaling. Because PV.1A is a critical downstream mediator of, and tightly regulated by, BMP-4 signaling, we hypothesized that its promoter contains a direct BMP-4 response element to effect this transcriptional regulation. We demonstrate that direct regulation by BMP-4 is necessary for transcription of PV.1A: its proximal promoter contains cis-acting binding elements for Smads and Oaz crucial to induction in response to BMP-4 signaling. In addition to these direct cis-acting BMP-4 responsive elements, an indirect Xvent-2 response element and several repressive elements exist in the PV.1A promoter to regulate its transcription. In summary, PV.1A undergoes combinatorial regulation during early Xenopus development as both the direct target of BMP-4 signaling and as the direct and indirect target of positive and negative regulatory factors.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0022621</identifier><identifier>PMID: 21857938</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>5' Flanking Region - genetics ; Animals ; Biochemistry ; Biology ; Bone morphogenetic protein 4 ; Bone Morphogenetic Protein 4 - genetics ; Bone Morphogenetic Protein 4 - metabolism ; Bone morphogenetic proteins ; Cloning ; Combinatorial analysis ; Cooperation ; Deoxyribonucleic acid ; DNA ; DNA binding proteins ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Electrophoretic Mobility Shift Assay ; Embryogenesis ; Embryonic development ; Embryonic growth stage ; GATA2 Transcription Factor - genetics ; GATA2 Transcription Factor - metabolism ; Gene expression ; Gene Expression Regulation, Developmental ; Gene regulation ; Genes ; Genetic aspects ; Homeobox ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Laboratory animals ; Medicine ; Molecular Sequence Data ; Mutation ; Promoter Regions, Genetic - genetics ; Promoters (Genetics) ; Protein Binding ; Proteins ; Regulatory sequences ; Reptiles & amphibians ; Response Elements - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Signaling ; Smad Proteins - genetics ; Smad Proteins - metabolism ; Transcription (Genetics) ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Xenopus ; Xenopus laevis - embryology ; Xenopus laevis - genetics ; Xenopus laevis - metabolism ; Xenopus Proteins - genetics ; Xenopus Proteins - metabolism</subject><ispartof>PloS one, 2011-08, Vol.6 (8), p.e22621</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Lee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Lee et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-6aade8f2d21fa023065386491b6c899d1cb422f304000b5d8df0d9d1e69501733</citedby><cites>FETCH-LOGICAL-c691t-6aade8f2d21fa023065386491b6c899d1cb422f304000b5d8df0d9d1e69501733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153937/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153937/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21857938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Hyun-Shik</creatorcontrib><creatorcontrib>Lee, Sung-Young</creatorcontrib><creatorcontrib>Lee, Hyosang</creatorcontrib><creatorcontrib>Hwang, Yoo-Seok</creatorcontrib><creatorcontrib>Cha, Sang-Wook</creatorcontrib><creatorcontrib>Park, Soochul</creatorcontrib><creatorcontrib>Lee, Jae-Yong</creatorcontrib><creatorcontrib>Park, Jae-Bong</creatorcontrib><creatorcontrib>Kim, Sungchan</creatorcontrib><creatorcontrib>Park, Mae Ja</creatorcontrib><creatorcontrib>Kim, Jaebong</creatorcontrib><title>Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Xvent homeobox genes encode transcription factors that repress organizer genes and are essential for dorsoventral specification during early embryogenesis in Xenopus. In contrast to the Xvent-2 gene subfamily, Xvent-1 subfamily members, including PV.1A, have been proposed as indirect targets of Bone Morphogenetic Protein-4 (BMP-4) signaling. Because PV.1A is a critical downstream mediator of, and tightly regulated by, BMP-4 signaling, we hypothesized that its promoter contains a direct BMP-4 response element to effect this transcriptional regulation. We demonstrate that direct regulation by BMP-4 is necessary for transcription of PV.1A: its proximal promoter contains cis-acting binding elements for Smads and Oaz crucial to induction in response to BMP-4 signaling. In addition to these direct cis-acting BMP-4 responsive elements, an indirect Xvent-2 response element and several repressive elements exist in the PV.1A promoter to regulate its transcription. In summary, PV.1A undergoes combinatorial regulation during early Xenopus development as both the direct target of BMP-4 signaling and as the direct and indirect target of positive and negative regulatory factors.</description><subject>5' Flanking Region - genetics</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biology</subject><subject>Bone morphogenetic protein 4</subject><subject>Bone Morphogenetic Protein 4 - genetics</subject><subject>Bone Morphogenetic Protein 4 - metabolism</subject><subject>Bone morphogenetic proteins</subject><subject>Cloning</subject><subject>Combinatorial analysis</subject><subject>Cooperation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA binding proteins</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Embryogenesis</subject><subject>Embryonic development</subject><subject>Embryonic growth stage</subject><subject>GATA2 Transcription Factor - genetics</subject><subject>GATA2 Transcription Factor - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Homeobox</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Laboratory animals</subject><subject>Medicine</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoters (Genetics)</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Regulatory sequences</subject><subject>Reptiles & amphibians</subject><subject>Response Elements - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Signal Transduction</subject><subject>Signaling</subject><subject>Smad Proteins - genetics</subject><subject>Smad Proteins - metabolism</subject><subject>Transcription (Genetics)</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Xenopus</subject><subject>Xenopus laevis - embryology</subject><subject>Xenopus laevis - genetics</subject><subject>Xenopus laevis - metabolism</subject><subject>Xenopus Proteins - genetics</subject><subject>Xenopus Proteins - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tu1DAQhiMEoqXwBggsIYG42MWOc7BvKi3ltFJRKw4Vd5Y3nux65cTBdgp9Bl4ap5tWG9QLlIskk2_-f2biSZKnBM8JLcmbre1dK828sy3MMU7TIiX3kkPCaTorUkzv7z0fJI-832KcU1YUD5ODlLC85JQdJn_eaQdVQA58FPKAwEADbfDI1ujt53P0S4eNblHYADq_mJMF6pxtbACHpIu09xHW0qDaOqRjWnCy9ZXTXdA2lheF172RwwtSvdPtGoF05gr9gNZ2vUcKLsHYbvB8nDyopfHwZLwfJd8_vP928ml2evZxebI4nVUFJ2FWSKmA1alKSS1xSnExtJVxsioqxrki1SpL05riDGO8yhVTNVYxDAXPMSkpPUqe73Q7Y70Y5-gFiUqM5yUbiOWOUFZuRed0I92VsFKL64B1ayFd0JUBUXKCScGYVLTOqhRzwJJk0VOWnPO6jFrHo1u_akBVsVEnzUR0-qXVG7G2l4KSnHI6CLwaBZz92YMPotG-AmNkC7b3grGMZQUuhrJf_EPe3dxIrWWsX7e1jbbVoCkWWRlbofTadX4HFS8Fja7imat1jE8SXk8SIhPgd1jL3nux_Prl_9mziyn7co_dgDRh463phyPlp2C2AytnvXdQ386YYDGszM00xLAyYlyZmPZs___cJt3sCP0Lzc4Scw</recordid><startdate>20110803</startdate><enddate>20110803</enddate><creator>Lee, Hyun-Shik</creator><creator>Lee, Sung-Young</creator><creator>Lee, Hyosang</creator><creator>Hwang, Yoo-Seok</creator><creator>Cha, Sang-Wook</creator><creator>Park, Soochul</creator><creator>Lee, Jae-Yong</creator><creator>Park, Jae-Bong</creator><creator>Kim, Sungchan</creator><creator>Park, Mae Ja</creator><creator>Kim, Jaebong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110803</creationdate><title>Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development</title><author>Lee, Hyun-Shik ; Lee, Sung-Young ; Lee, Hyosang ; Hwang, Yoo-Seok ; Cha, Sang-Wook ; Park, Soochul ; Lee, Jae-Yong ; Park, Jae-Bong ; Kim, Sungchan ; Park, Mae Ja ; Kim, Jaebong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-6aade8f2d21fa023065386491b6c899d1cb422f304000b5d8df0d9d1e69501733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>5' Flanking Region - genetics</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biology</topic><topic>Bone morphogenetic protein 4</topic><topic>Bone Morphogenetic Protein 4 - genetics</topic><topic>Bone Morphogenetic Protein 4 - metabolism</topic><topic>Bone morphogenetic proteins</topic><topic>Cloning</topic><topic>Combinatorial analysis</topic><topic>Cooperation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA binding proteins</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Embryogenesis</topic><topic>Embryonic development</topic><topic>Embryonic growth stage</topic><topic>GATA2 Transcription Factor - genetics</topic><topic>GATA2 Transcription Factor - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Homeobox</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Laboratory animals</topic><topic>Medicine</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoters (Genetics)</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Regulatory sequences</topic><topic>Reptiles & amphibians</topic><topic>Response Elements - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Signal Transduction</topic><topic>Signaling</topic><topic>Smad Proteins - genetics</topic><topic>Smad Proteins - metabolism</topic><topic>Transcription (Genetics)</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Xenopus</topic><topic>Xenopus laevis - embryology</topic><topic>Xenopus laevis - genetics</topic><topic>Xenopus laevis - metabolism</topic><topic>Xenopus Proteins - genetics</topic><topic>Xenopus Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Hyun-Shik</creatorcontrib><creatorcontrib>Lee, Sung-Young</creatorcontrib><creatorcontrib>Lee, Hyosang</creatorcontrib><creatorcontrib>Hwang, Yoo-Seok</creatorcontrib><creatorcontrib>Cha, Sang-Wook</creatorcontrib><creatorcontrib>Park, Soochul</creatorcontrib><creatorcontrib>Lee, Jae-Yong</creatorcontrib><creatorcontrib>Park, Jae-Bong</creatorcontrib><creatorcontrib>Kim, Sungchan</creatorcontrib><creatorcontrib>Park, Mae Ja</creatorcontrib><creatorcontrib>Kim, Jaebong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Hyun-Shik</au><au>Lee, Sung-Young</au><au>Lee, Hyosang</au><au>Hwang, Yoo-Seok</au><au>Cha, Sang-Wook</au><au>Park, Soochul</au><au>Lee, Jae-Yong</au><au>Park, Jae-Bong</au><au>Kim, Sungchan</au><au>Park, Mae Ja</au><au>Kim, Jaebong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-08-03</date><risdate>2011</risdate><volume>6</volume><issue>8</issue><spage>e22621</spage><pages>e22621-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Xvent homeobox genes encode transcription factors that repress organizer genes and are essential for dorsoventral specification during early embryogenesis in Xenopus. In contrast to the Xvent-2 gene subfamily, Xvent-1 subfamily members, including PV.1A, have been proposed as indirect targets of Bone Morphogenetic Protein-4 (BMP-4) signaling. Because PV.1A is a critical downstream mediator of, and tightly regulated by, BMP-4 signaling, we hypothesized that its promoter contains a direct BMP-4 response element to effect this transcriptional regulation. We demonstrate that direct regulation by BMP-4 is necessary for transcription of PV.1A: its proximal promoter contains cis-acting binding elements for Smads and Oaz crucial to induction in response to BMP-4 signaling. In addition to these direct cis-acting BMP-4 responsive elements, an indirect Xvent-2 response element and several repressive elements exist in the PV.1A promoter to regulate its transcription. In summary, PV.1A undergoes combinatorial regulation during early Xenopus development as both the direct target of BMP-4 signaling and as the direct and indirect target of positive and negative regulatory factors.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21857938</pmid><doi>10.1371/journal.pone.0022621</doi><tpages>e22621</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2011-08, Vol.6 (8), p.e22621 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1306895783 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 5' Flanking Region - genetics Animals Biochemistry Biology Bone morphogenetic protein 4 Bone Morphogenetic Protein 4 - genetics Bone Morphogenetic Protein 4 - metabolism Bone morphogenetic proteins Cloning Combinatorial analysis Cooperation Deoxyribonucleic acid DNA DNA binding proteins DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Electrophoretic Mobility Shift Assay Embryogenesis Embryonic development Embryonic growth stage GATA2 Transcription Factor - genetics GATA2 Transcription Factor - metabolism Gene expression Gene Expression Regulation, Developmental Gene regulation Genes Genetic aspects Homeobox Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Laboratory animals Medicine Molecular Sequence Data Mutation Promoter Regions, Genetic - genetics Promoters (Genetics) Protein Binding Proteins Regulatory sequences Reptiles & amphibians Response Elements - genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Signaling Smad Proteins - genetics Smad Proteins - metabolism Transcription (Genetics) Transcription factors Transcription Factors - genetics Transcription Factors - metabolism Xenopus Xenopus laevis - embryology Xenopus laevis - genetics Xenopus laevis - metabolism Xenopus Proteins - genetics Xenopus Proteins - metabolism |
| title | Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T22%3A44%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20response%20elements%20of%20BMP%20within%20the%20PV.1A%20promoter%20are%20essential%20for%20its%20transcriptional%20regulation%20during%20early%20Xenopus%20development&rft.jtitle=PloS%20one&rft.au=Lee,%20Hyun-Shik&rft.date=2011-08-03&rft.volume=6&rft.issue=8&rft.spage=e22621&rft.pages=e22621-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0022621&rft_dat=%3Cgale_plos_%3EA476883337%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1306895783&rft_id=info:pmid/21857938&rft_galeid=A476883337&rft_doaj_id=oai_doaj_org_article_79101688ad3f4c209e0a148dfa7999f7&rfr_iscdi=true |