Quantitative photo activated localization microscopy: unraveling the effects of photoblinking

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...

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Veröffentlicht in:	PloS one 2011-07, Vol.6 (7), p.e22678-e22678
Hauptverfasser:	Annibale, Paolo, Vanni, Stefano, Scarselli, Marco, Rothlisberger, Ursula, Radenovic, Aleksandra
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenergic receptors Biochemistry Bioengineering Biological samples Biology Computational chemistry Computer Simulation Gene expression HeLa Cells Humans Information management Laboratories Light Localization Luminescent Proteins - metabolism Microscopy Microscopy - methods Palm Physical chemistry Physics Proteins Receptors, Adrenergic, beta-2 - metabolism Science
Online-Zugang:	Volltext
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