Quantitative photo activated localization microscopy: unraveling the effects of photoblinking
In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way...
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| Veröffentlicht in: | PloS one 2011-07, Vol.6 (7), p.e22678-e22678 |
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| creator | Annibale, Paolo Vanni, Stefano Scarselli, Marco Rothlisberger, Ursula Radenovic, Aleksandra |
| description | In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells. |
| doi_str_mv | 10.1371/journal.pone.0022678 |
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| subjects | Adrenergic receptors Biochemistry Bioengineering Biological samples Biology Computational chemistry Computer Simulation Gene expression HeLa Cells Humans Information management Laboratories Light Localization Luminescent Proteins - metabolism Microscopy Microscopy - methods Palm Physical chemistry Physics Proteins Receptors, Adrenergic, beta-2 - metabolism Science |
| title | Quantitative photo activated localization microscopy: unraveling the effects of photoblinking |
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