Assessing the permeability of engineered capillary networks in a 3D culture

Many pathologies are characterized by poor blood vessel growth and reduced nutrient delivery to the surrounding tissue, introducing a need for tissue engineered blood vessels. Our lab has developed a 3D co-culture method to grow interconnected networks of pericyte-invested capillaries, which can ana...

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Veröffentlicht in:	PloS one 2011-07, Vol.6 (7), p.e22086-e22086
Hauptverfasser:	Grainger, Stephanie J, Putnam, Andrew J
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Angiogenesis Biochemistry Biological Assay Biology Biomedical engineering Blood Blood flow Blood vessels Bone marrow Cadherins Cadherins - metabolism Cancer Capillaries Capillaries - metabolism Capillary Permeability Cell culture Cell Proliferation Dextran Dextrans Dextrans - metabolism Endothelial cells Endothelial Cells - cytology Endothelial Cells - metabolism Endothelium Engineering Extracellular matrix Fibroblasts Fluorescence Histamine - metabolism Humans Implantation Ischemia Kinetics Laboratories Medicine Membrane permeability Mesenchymal stem cells Mesenchyme Microscopy, Confocal Models, Biological Musculoskeletal system Neovascularization Networks Penicillin Permeability Physiology Reproducibility of Results Stem cells Stem Cells - cytology Stem Cells - metabolism Stromal Cells - metabolism Tissue Culture Techniques Tissue Engineering - methods Tissues Tracers (Biology) Tumors
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