Radioactive phosphorylation of alcohols to monitor biocatalytic Diels-Alder reactions

Nature has efficiently adopted phosphorylation for numerous biological key processes, spanning from cell signaling to energy storage and transmission. For the bioorganic chemist the number of possible ways to attach a single phosphate for radioactive labeling is surprisingly small. Here we describe...

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Veröffentlicht in:	PloS one 2011-06, Vol.6 (6), p.e21391-e21391
Hauptverfasser:	Nierth, Alexander, Jäschke, Andres
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Alcohol Alcoholic beverages Alcohols Alcohols - metabolism Analysis Anthracene Anthracenes - chemistry Anthracenes - metabolism Biocatalysis Biocatalysts Biological activity Biological Assay Biology Catalysis Catalytic RNA Chemical industry Chemical kinetics Chemistry Chemistry, Organic - methods Chromatography Dehydrogenases Densitometers Diels-Alder reactions Dyes Energy storage Energy transmission Enzymes Fluorescence Fluorescence Resonance Energy Transfer Hydroxyl groups Hypoxia Kinetics Labeling Labelling Models, Chemical Nucleic Acid Conformation Nucleotide sequence Optical activity Optimization Pharmacy Phosphate esters Phosphates Phosphoric acid Phosphorus Phosphorus Radioisotopes Phosphorylation Proteins Radioactive labeling Reaction kinetics Ribonucleic acid RNA RNA, Catalytic - chemistry RNA, Catalytic - metabolism Signaling Substrate Specificity
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