A novel SERRS sandwich-hybridization assay to detect specific DNA target
In this study, we have applied Surface Enhanced Resonance Raman Scattering (SERRS) technology to the specific detection of DNA. We present an innovative SERRS sandwich-hybridization assay that allows specific DNA detection without any enzymatic amplification, such as is the case with Polymerase Chai...
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| Veröffentlicht in: | PloS one 2011-05, Vol.6 (5), p.e17847-e17847 |
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| creator | Feuillie, Cécile Merheb, Maxime Mohamad Gillet, Benjamin Montagnac, Gilles Daniel, Isabelle Hänni, Catherine |
| description | In this study, we have applied Surface Enhanced Resonance Raman Scattering (SERRS) technology to the specific detection of DNA. We present an innovative SERRS sandwich-hybridization assay that allows specific DNA detection without any enzymatic amplification, such as is the case with Polymerase Chain Reaction (PCR). In some substrates, such as ancient or processed remains, enzymatic amplification fails due to DNA alteration (degradation, chemical modification) or to the presence of inhibitors. Consequently, the development of a non-enzymatic method, allowing specific DNA detection, could avoid long, expensive and inconclusive amplification trials. Here, we report the proof of concept of a SERRS sandwich-hybridization assay that leads to the detection of a specific chamois DNA. This SERRS assay reveals its potential as a non-enzymatic alternative technology to DNA amplification methods (particularly the PCR method) with several applications for species detection. As the amount and type of damage highly depend on the preservation conditions, the present SERRS assay would enlarge the range of samples suitable for DNA analysis and ultimately would provide exciting new opportunities for the investigation of ancient DNA in the fields of evolutionary biology and molecular ecology, and of altered DNA in food frauds detection and forensics. |
| doi_str_mv | 10.1371/journal.pone.0017847 |
| format | Article |
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We present an innovative SERRS sandwich-hybridization assay that allows specific DNA detection without any enzymatic amplification, such as is the case with Polymerase Chain Reaction (PCR). In some substrates, such as ancient or processed remains, enzymatic amplification fails due to DNA alteration (degradation, chemical modification) or to the presence of inhibitors. Consequently, the development of a non-enzymatic method, allowing specific DNA detection, could avoid long, expensive and inconclusive amplification trials. Here, we report the proof of concept of a SERRS sandwich-hybridization assay that leads to the detection of a specific chamois DNA. This SERRS assay reveals its potential as a non-enzymatic alternative technology to DNA amplification methods (particularly the PCR method) with several applications for species detection. 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| subjects | Alternative technology Amplification Analysis Assaying Biological evolution Biology Capra hircus Chemical modification Damage detection Deoxyribonucleic acid DNA DNA - analysis DNA damage Earth Sciences Ecology Enzymes Evolutionary biology Forensic science Genetic research Hybridization Influenza Methods Molecular biology Nucleic Acid Hybridization - methods Polymerase Chain Reaction Preservation Raman scattering Raman spectra Resonance scattering Rupicapra rupicapra Sciences of the Universe Spectrum Analysis, Raman Substrates Target detection Technology |
| title | A novel SERRS sandwich-hybridization assay to detect specific DNA target |
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