Circular permutation of red fluorescent proteins

Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) b...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2011-05, Vol.6 (5), p.e20505-e20505
Hauptverfasser:	Shui, Bo, Wang, Qi, Lee, Frank, Byrnes, Laura J, Chudakov, Dmitry M, Lukyanov, Sergey A, Sondermann, Holger, Kotlikoff, Michael I
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Biology Chemistry Chromophores Circularity Color Conformation Crystallography, X-Ray Discosoma Evaluation Fluorescence Luminescent Proteins - chemistry Luminescent Proteins - metabolism Materials Science Medicine Models, Molecular Molecular Sequence Data Mutation Peptides Physics Physiology Plasmids Protein Structure, Secondary Proteins Quantitative analysis Red Fluorescent Protein Spectrometry, Fluorescence Veterinary colleges Veterinary medicine
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!