Multiple-locus variable number tandem repeat analysis for Streptococcus pneumoniae: comparison with PFGE and MLST

In the era of pneumococcal conjugate vaccines, surveillance of pneumococcal disease and carriage remains of utmost importance as important changes may occur in the population. To monitor these alterations reliable genotyping methods are required for large-scale applications. We introduced a high thr...

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Veröffentlicht in:	PloS one 2011-05, Vol.6 (5), p.e19668-e19668
Hauptverfasser:	Elberse, Karin E M, Nunes, Sónia, Sá-Leão, Raquel, van der Heide, Han G J, Schouls, Leo M
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Typing Techniques - methods Biology Child Clustering Confidence intervals Day care centers Deoxyribonucleic acid Diversity indices DNA DNA sequencing Drug resistance Electrophoresis, Gel, Pulsed-Field - methods Epidemiology Gel electrophoresis Genetic diversity Genetic Loci - genetics Genome, Bacterial - genetics Genotyping Health surveillance Humans Infectious diseases Laboratories Loci Methods Microorganisms Minisatellite Repeats - genetics Multilocus sequence typing Multilocus Sequence Typing - methods Multiplexing Nucleotide sequence Pneumonia Population structure Public health Pulsed-field gel electrophoresis Serotypes Serotyping Streptococcus infections Streptococcus pneumoniae Streptococcus pneumoniae - classification Streptococcus pneumoniae - genetics Streptococcus pneumoniae - isolation & purification Streptococcus pyogenes Vaccines
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