Towards a synthetic chloroplast
The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic relationships between different spe...
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| Veröffentlicht in: | PloS one 2011-04, Vol.6 (4), p.e18877-e18877 |
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| creator | Agapakis, Christina M Niederholtmeyer, Henrike Noche, Ramil R Lieberman, Tami D Megason, Sean G Way, Jeffrey C Silver, Pamela A |
| description | The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic relationships between different species of cells are a valuable tool for synthetic biology, where engineered pathways based on two species could take advantage of the unique abilities of each mutualistic partner.
We explored the possibility of using the photosynthetic bacterium Synechococcus elongatus PCC 7942 as a platform for studying evolutionary dynamics and for designing two-species synthetic biological systems. We observed that the cyanobacteria were relatively harmless to eukaryotic host cells compared to Escherichia coli when injected into the embryos of zebrafish, Danio rerio, or taken up by mammalian macrophages. In addition, when engineered with invasin from Yersinia pestis and listeriolysin O from Listeria monocytogenes, S. elongatus was able to invade cultured mammalian cells and divide inside macrophages.
Our results show that it is possible to engineer photosynthetic bacteria to invade the cytoplasm of mammalian cells for further engineering and applications in synthetic biology. Engineered invasive but non-pathogenic or immunogenic photosynthetic bacteria have great potential as synthetic biological devices. |
| doi_str_mv | 10.1371/journal.pone.0018877 |
| format | Article |
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We explored the possibility of using the photosynthetic bacterium Synechococcus elongatus PCC 7942 as a platform for studying evolutionary dynamics and for designing two-species synthetic biological systems. We observed that the cyanobacteria were relatively harmless to eukaryotic host cells compared to Escherichia coli when injected into the embryos of zebrafish, Danio rerio, or taken up by mammalian macrophages. In addition, when engineered with invasin from Yersinia pestis and listeriolysin O from Listeria monocytogenes, S. elongatus was able to invade cultured mammalian cells and divide inside macrophages.
Our results show that it is possible to engineer photosynthetic bacteria to invade the cytoplasm of mammalian cells for further engineering and applications in synthetic biology. Engineered invasive but non-pathogenic or immunogenic photosynthetic bacteria have great potential as synthetic biological devices.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0018877</identifier><identifier>PMID: 21533097</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Algae ; Animal behavior ; Animals ; Bacteria ; Bacterial infections ; Bacterial Proteins - genetics ; Base Sequence ; Bioengineering ; Biological evolution ; Biology ; Chlorophyta ; Chloroplasts ; Consortia ; Cooperation ; Cyanobacteria ; Cytoplasm ; Danio rerio ; DNA - genetics ; DNA Primers ; E coli ; Elongation ; Embryonic development ; Embryos ; Engineering ; Escherichia coli ; Evolution, Molecular ; Gene therapy ; Genetic Engineering ; Genomes ; Immunogenicity ; Infections ; Listeria ; Listeria monocytogenes ; Listeriolysin O ; Macrophages ; Macrophages - microbiology ; Mammalian cells ; Mammals ; Medical schools ; Metabolism ; Metabolites ; Mitochondria ; Motility ; Photosynthesis ; Plasmids ; Species ; Symbiosis ; Synechococcus - genetics ; Synechococcus - growth & development ; Synechococcus - physiology ; Synthetic biology ; Trends ; Zebrafish ; Zebrafish - embryology</subject><ispartof>PloS one, 2011-04, Vol.6 (4), p.e18877-e18877</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Agapakis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Agapakis et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-417dc1d5efb158d822a0d0ca4dfcb62520482f1f3e4f017aa9274ecb362edc943</citedby><cites>FETCH-LOGICAL-c691t-417dc1d5efb158d822a0d0ca4dfcb62520482f1f3e4f017aa9274ecb362edc943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080389/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3080389/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21533097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ojcius, David M.</contributor><creatorcontrib>Agapakis, Christina M</creatorcontrib><creatorcontrib>Niederholtmeyer, Henrike</creatorcontrib><creatorcontrib>Noche, Ramil R</creatorcontrib><creatorcontrib>Lieberman, Tami D</creatorcontrib><creatorcontrib>Megason, Sean G</creatorcontrib><creatorcontrib>Way, Jeffrey C</creatorcontrib><creatorcontrib>Silver, Pamela A</creatorcontrib><title>Towards a synthetic chloroplast</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic relationships between different species of cells are a valuable tool for synthetic biology, where engineered pathways based on two species could take advantage of the unique abilities of each mutualistic partner.
We explored the possibility of using the photosynthetic bacterium Synechococcus elongatus PCC 7942 as a platform for studying evolutionary dynamics and for designing two-species synthetic biological systems. We observed that the cyanobacteria were relatively harmless to eukaryotic host cells compared to Escherichia coli when injected into the embryos of zebrafish, Danio rerio, or taken up by mammalian macrophages. In addition, when engineered with invasin from Yersinia pestis and listeriolysin O from Listeria monocytogenes, S. elongatus was able to invade cultured mammalian cells and divide inside macrophages.
Our results show that it is possible to engineer photosynthetic bacteria to invade the cytoplasm of mammalian cells for further engineering and applications in synthetic biology. 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genetics</topic><topic>Base Sequence</topic><topic>Bioengineering</topic><topic>Biological evolution</topic><topic>Biology</topic><topic>Chlorophyta</topic><topic>Chloroplasts</topic><topic>Consortia</topic><topic>Cooperation</topic><topic>Cyanobacteria</topic><topic>Cytoplasm</topic><topic>Danio rerio</topic><topic>DNA - genetics</topic><topic>DNA Primers</topic><topic>E coli</topic><topic>Elongation</topic><topic>Embryonic development</topic><topic>Embryos</topic><topic>Engineering</topic><topic>Escherichia coli</topic><topic>Evolution, Molecular</topic><topic>Gene therapy</topic><topic>Genetic Engineering</topic><topic>Genomes</topic><topic>Immunogenicity</topic><topic>Infections</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeriolysin O</topic><topic>Macrophages</topic><topic>Macrophages - microbiology</topic><topic>Mammalian cells</topic><topic>Mammals</topic><topic>Medical schools</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Mitochondria</topic><topic>Motility</topic><topic>Photosynthesis</topic><topic>Plasmids</topic><topic>Species</topic><topic>Symbiosis</topic><topic>Synechococcus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Agapakis, Christina M</au><au>Niederholtmeyer, Henrike</au><au>Noche, Ramil R</au><au>Lieberman, Tami D</au><au>Megason, Sean G</au><au>Way, Jeffrey C</au><au>Silver, Pamela A</au><au>Ojcius, David M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Towards a synthetic chloroplast</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-04-20</date><risdate>2011</risdate><volume>6</volume><issue>4</issue><spage>e18877</spage><epage>e18877</epage><pages>e18877-e18877</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic relationships between different species of cells are a valuable tool for synthetic biology, where engineered pathways based on two species could take advantage of the unique abilities of each mutualistic partner.
We explored the possibility of using the photosynthetic bacterium Synechococcus elongatus PCC 7942 as a platform for studying evolutionary dynamics and for designing two-species synthetic biological systems. We observed that the cyanobacteria were relatively harmless to eukaryotic host cells compared to Escherichia coli when injected into the embryos of zebrafish, Danio rerio, or taken up by mammalian macrophages. In addition, when engineered with invasin from Yersinia pestis and listeriolysin O from Listeria monocytogenes, S. elongatus was able to invade cultured mammalian cells and divide inside macrophages.
Our results show that it is possible to engineer photosynthetic bacteria to invade the cytoplasm of mammalian cells for further engineering and applications in synthetic biology. Engineered invasive but non-pathogenic or immunogenic photosynthetic bacteria have great potential as synthetic biological devices.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21533097</pmid><doi>10.1371/journal.pone.0018877</doi><tpages>e18877</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Algae Animal behavior Animals Bacteria Bacterial infections Bacterial Proteins - genetics Base Sequence Bioengineering Biological evolution Biology Chlorophyta Chloroplasts Consortia Cooperation Cyanobacteria Cytoplasm Danio rerio DNA - genetics DNA Primers E coli Elongation Embryonic development Embryos Engineering Escherichia coli Evolution, Molecular Gene therapy Genetic Engineering Genomes Immunogenicity Infections Listeria Listeria monocytogenes Listeriolysin O Macrophages Macrophages - microbiology Mammalian cells Mammals Medical schools Metabolism Metabolites Mitochondria Motility Photosynthesis Plasmids Species Symbiosis Synechococcus - genetics Synechococcus - growth & development Synechococcus - physiology Synthetic biology Trends Zebrafish Zebrafish - embryology |
| title | Towards a synthetic chloroplast |
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