Distinct cerebrospinal fluid proteomes differentiate post-treatment lyme disease from chronic fatigue syndrome
Neurologic Post Treatment Lyme disease (nPTLS) and Chronic Fatigue (CFS) are syndromes of unknown etiology. They share features of fatigue and cognitive dysfunction, making it difficult to differentiate them. Unresolved is whether nPTLS is a subset of CFS. Pooled cerebrospinal fluid (CSF) samples fr...
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| Veröffentlicht in: | PloS one 2011-02, Vol.6 (2), p.e17287-e17287 |
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| creator | Schutzer, Steven E Angel, Thomas E Liu, Tao Schepmoes, Athena A Clauss, Therese R Adkins, Joshua N Camp, David G Holland, Bart K Bergquist, Jonas Coyle, Patricia K Smith, Richard D Fallon, Brian A Natelson, Benjamin H |
| description | Neurologic Post Treatment Lyme disease (nPTLS) and Chronic Fatigue (CFS) are syndromes of unknown etiology. They share features of fatigue and cognitive dysfunction, making it difficult to differentiate them. Unresolved is whether nPTLS is a subset of CFS.
Pooled cerebrospinal fluid (CSF) samples from nPTLS patients, CFS patients, and healthy volunteers were comprehensively analyzed using high-resolution mass spectrometry (MS), coupled with immunoaffinity depletion methods to reduce protein-masking by abundant proteins. Individual patient and healthy control CSF samples were analyzed directly employing a MS-based label-free quantitative proteomics approach. We found that both groups, and individuals within the groups, could be distinguished from each other and normals based on their specific CSF proteins (p |
| doi_str_mv | 10.1371/journal.pone.0017287 |
| format | Article |
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Pooled cerebrospinal fluid (CSF) samples from nPTLS patients, CFS patients, and healthy volunteers were comprehensively analyzed using high-resolution mass spectrometry (MS), coupled with immunoaffinity depletion methods to reduce protein-masking by abundant proteins. Individual patient and healthy control CSF samples were analyzed directly employing a MS-based label-free quantitative proteomics approach. We found that both groups, and individuals within the groups, could be distinguished from each other and normals based on their specific CSF proteins (p<0.01). CFS (n = 43) had 2,783 non-redundant proteins, nPTLS (n = 25) contained 2,768 proteins, and healthy normals had 2,630 proteins. Preliminary pathway analysis demonstrated that the data could be useful for hypothesis generation on the pathogenetic mechanisms underlying these two related syndromes.
nPTLS and CFS have distinguishing CSF protein complements. Each condition has a number of CSF proteins that can be useful in providing candidates for future validation studies and insights on the respective mechanisms of pathogenesis. Distinguishing nPTLS and CFS permits more focused study of each condition, and can lead to novel diagnostics and therapeutic interventions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0017287</identifier><identifier>PMID: 21383843</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Biology ; Biomarkers ; Brain research ; Case-Control Studies ; Cerebrospinal fluid ; Cerebrospinal fluid proteins ; Cerebrospinal Fluid Proteins - analysis ; Cerebrospinal Fluid Proteins - metabolism ; Chronic fatigue syndrome ; Cognitive ability ; Cognitive disorders ; Dentistry ; Development and progression ; Diagnosis, Differential ; Disorders ; Drug therapy ; Etiology ; Exigent circumstances ; Fatigue ; Fatigue Syndrome, Chronic - cerebrospinal fluid ; Fatigue Syndrome, Chronic - diagnosis ; Fatigue Syndrome, Chronic - metabolism ; Fatigue Syndrome, Chronic - therapy ; Female ; Forensic sciences ; Humans ; Laboratories ; Lyme disease ; Lyme Disease - cerebrospinal fluid ; Lyme Disease - diagnosis ; Lyme Disease - metabolism ; Lyme Disease - therapy ; Male ; Masking ; Mass spectrometry ; Mass spectroscopy ; Medical treatment ; MEDICIN ; MEDICINE ; Middle Aged ; Parkinson's disease ; Parkinsons disease ; Pathogenesis ; Patients ; Proteins ; Proteome - analysis ; Proteomics ; Proteomics - methods ; Quantitative analysis ; Scientific imaging ; Therapeutic applications ; Treatment Outcome ; Vector-borne diseases ; Young Adult</subject><ispartof>PloS one, 2011-02, Vol.6 (2), p.e17287-e17287</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Schutzer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Schutzer et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c755t-b1a8e2a3715909355e89501a8ffa933fce923fd818ee2f8a226b173c547317773</citedby><cites>FETCH-LOGICAL-c755t-b1a8e2a3715909355e89501a8ffa933fce923fd818ee2f8a226b173c547317773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044169/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3044169/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23847,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21383843$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1152617$$D View this record in Osti.gov$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-148991$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><contributor>Gendelman, Howard</contributor><creatorcontrib>Schutzer, Steven E</creatorcontrib><creatorcontrib>Angel, Thomas E</creatorcontrib><creatorcontrib>Liu, Tao</creatorcontrib><creatorcontrib>Schepmoes, Athena A</creatorcontrib><creatorcontrib>Clauss, Therese R</creatorcontrib><creatorcontrib>Adkins, Joshua N</creatorcontrib><creatorcontrib>Camp, David G</creatorcontrib><creatorcontrib>Holland, Bart K</creatorcontrib><creatorcontrib>Bergquist, Jonas</creatorcontrib><creatorcontrib>Coyle, Patricia K</creatorcontrib><creatorcontrib>Smith, Richard D</creatorcontrib><creatorcontrib>Fallon, Brian A</creatorcontrib><creatorcontrib>Natelson, Benjamin H</creatorcontrib><creatorcontrib>Environmental Molecular Sciences Laboratory (EMSL)</creatorcontrib><title>Distinct cerebrospinal fluid proteomes differentiate post-treatment lyme disease from chronic fatigue syndrome</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Neurologic Post Treatment Lyme disease (nPTLS) and Chronic Fatigue (CFS) are syndromes of unknown etiology. They share features of fatigue and cognitive dysfunction, making it difficult to differentiate them. Unresolved is whether nPTLS is a subset of CFS.
Pooled cerebrospinal fluid (CSF) samples from nPTLS patients, CFS patients, and healthy volunteers were comprehensively analyzed using high-resolution mass spectrometry (MS), coupled with immunoaffinity depletion methods to reduce protein-masking by abundant proteins. Individual patient and healthy control CSF samples were analyzed directly employing a MS-based label-free quantitative proteomics approach. We found that both groups, and individuals within the groups, could be distinguished from each other and normals based on their specific CSF proteins (p<0.01). CFS (n = 43) had 2,783 non-redundant proteins, nPTLS (n = 25) contained 2,768 proteins, and healthy normals had 2,630 proteins. Preliminary pathway analysis demonstrated that the data could be useful for hypothesis generation on the pathogenetic mechanisms underlying these two related syndromes.
nPTLS and CFS have distinguishing CSF protein complements. Each condition has a number of CSF proteins that can be useful in providing candidates for future validation studies and insights on the respective mechanisms of pathogenesis. Distinguishing nPTLS and CFS permits more focused study of each condition, and can lead to novel diagnostics and therapeutic interventions.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Brain research</subject><subject>Case-Control Studies</subject><subject>Cerebrospinal fluid</subject><subject>Cerebrospinal fluid proteins</subject><subject>Cerebrospinal Fluid Proteins - analysis</subject><subject>Cerebrospinal Fluid Proteins - metabolism</subject><subject>Chronic fatigue syndrome</subject><subject>Cognitive ability</subject><subject>Cognitive disorders</subject><subject>Dentistry</subject><subject>Development and progression</subject><subject>Diagnosis, Differential</subject><subject>Disorders</subject><subject>Drug therapy</subject><subject>Etiology</subject><subject>Exigent circumstances</subject><subject>Fatigue</subject><subject>Fatigue Syndrome, Chronic - cerebrospinal fluid</subject><subject>Fatigue Syndrome, Chronic - diagnosis</subject><subject>Fatigue Syndrome, Chronic - metabolism</subject><subject>Fatigue Syndrome, Chronic - therapy</subject><subject>Female</subject><subject>Forensic sciences</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Lyme disease</subject><subject>Lyme Disease - cerebrospinal fluid</subject><subject>Lyme Disease - diagnosis</subject><subject>Lyme Disease - metabolism</subject><subject>Lyme Disease - therapy</subject><subject>Male</subject><subject>Masking</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical treatment</subject><subject>MEDICIN</subject><subject>MEDICINE</subject><subject>Middle Aged</subject><subject>Parkinson's disease</subject><subject>Parkinsons disease</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>Proteomics</subject><subject>Proteomics - 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titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Uppsala universitet</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schutzer, Steven E</au><au>Angel, Thomas E</au><au>Liu, Tao</au><au>Schepmoes, Athena A</au><au>Clauss, Therese R</au><au>Adkins, Joshua N</au><au>Camp, David G</au><au>Holland, Bart K</au><au>Bergquist, Jonas</au><au>Coyle, Patricia K</au><au>Smith, Richard D</au><au>Fallon, Brian A</au><au>Natelson, Benjamin H</au><au>Gendelman, Howard</au><aucorp>Environmental Molecular Sciences Laboratory (EMSL)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinct cerebrospinal fluid proteomes differentiate post-treatment lyme disease from chronic fatigue syndrome</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-02-23</date><risdate>2011</risdate><volume>6</volume><issue>2</issue><spage>e17287</spage><epage>e17287</epage><pages>e17287-e17287</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Neurologic Post Treatment Lyme disease (nPTLS) and Chronic Fatigue (CFS) are syndromes of unknown etiology. They share features of fatigue and cognitive dysfunction, making it difficult to differentiate them. Unresolved is whether nPTLS is a subset of CFS.
Pooled cerebrospinal fluid (CSF) samples from nPTLS patients, CFS patients, and healthy volunteers were comprehensively analyzed using high-resolution mass spectrometry (MS), coupled with immunoaffinity depletion methods to reduce protein-masking by abundant proteins. Individual patient and healthy control CSF samples were analyzed directly employing a MS-based label-free quantitative proteomics approach. We found that both groups, and individuals within the groups, could be distinguished from each other and normals based on their specific CSF proteins (p<0.01). CFS (n = 43) had 2,783 non-redundant proteins, nPTLS (n = 25) contained 2,768 proteins, and healthy normals had 2,630 proteins. Preliminary pathway analysis demonstrated that the data could be useful for hypothesis generation on the pathogenetic mechanisms underlying these two related syndromes.
nPTLS and CFS have distinguishing CSF protein complements. Each condition has a number of CSF proteins that can be useful in providing candidates for future validation studies and insights on the respective mechanisms of pathogenesis. Distinguishing nPTLS and CFS permits more focused study of each condition, and can lead to novel diagnostics and therapeutic interventions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21383843</pmid><doi>10.1371/journal.pone.0017287</doi><tpages>e17287</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1296428422 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adolescent Adult Biology Biomarkers Brain research Case-Control Studies Cerebrospinal fluid Cerebrospinal fluid proteins Cerebrospinal Fluid Proteins - analysis Cerebrospinal Fluid Proteins - metabolism Chronic fatigue syndrome Cognitive ability Cognitive disorders Dentistry Development and progression Diagnosis, Differential Disorders Drug therapy Etiology Exigent circumstances Fatigue Fatigue Syndrome, Chronic - cerebrospinal fluid Fatigue Syndrome, Chronic - diagnosis Fatigue Syndrome, Chronic - metabolism Fatigue Syndrome, Chronic - therapy Female Forensic sciences Humans Laboratories Lyme disease Lyme Disease - cerebrospinal fluid Lyme Disease - diagnosis Lyme Disease - metabolism Lyme Disease - therapy Male Masking Mass spectrometry Mass spectroscopy Medical treatment MEDICIN MEDICINE Middle Aged Parkinson's disease Parkinsons disease Pathogenesis Patients Proteins Proteome - analysis Proteomics Proteomics - methods Quantitative analysis Scientific imaging Therapeutic applications Treatment Outcome Vector-borne diseases Young Adult |
| title | Distinct cerebrospinal fluid proteomes differentiate post-treatment lyme disease from chronic fatigue syndrome |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T04%3A48%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distinct%20cerebrospinal%20fluid%20proteomes%20differentiate%20post-treatment%20lyme%20disease%20from%20chronic%20fatigue%20syndrome&rft.jtitle=PloS%20one&rft.au=Schutzer,%20Steven%20E&rft.aucorp=Environmental%20Molecular%20Sciences%20Laboratory%20(EMSL)&rft.date=2011-02-23&rft.volume=6&rft.issue=2&rft.spage=e17287&rft.epage=e17287&rft.pages=e17287-e17287&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0017287&rft_dat=%3Cgale_plos_%3EA476903843%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1296428422&rft_id=info:pmid/21383843&rft_galeid=A476903843&rft_doaj_id=oai_doaj_org_article_289b8e3f2af942ad86653c771d9bda42&rfr_iscdi=true |