The role of neutrophils during mild and severe influenza virus infections of mice
Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also...
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| description | Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1(+) cells, not neutrophils, limit disease severity during mild influenza infections. |
| doi_str_mv | 10.1371/journal.pone.0017618 |
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We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1(+) cells, not neutrophils, limit disease severity during mild influenza infections.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0017618</identifier><identifier>PMID: 21423798</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Administration, Intranasal ; Animals ; Antibodies, Monoclonal - pharmacology ; Avian flu ; Biology ; Bone marrow ; Disease control ; Granulocytes ; Health aspects ; Immunology ; Infections ; Influenza ; Influenza virus ; Influenza viruses ; Lectins ; Leukocytes ; Leukocytes (neutrophilic) ; Lungs ; Medical treatment ; Medicine ; Mice ; Mice, Inbred C57BL ; Neutropenia ; Neutrophil Infiltration - drug effects ; Neutrophils ; Neutrophils - drug effects ; Neutrophils - immunology ; Neutrophils - virology ; Orthomyxoviridae - drug effects ; Orthomyxoviridae - pathogenicity ; Orthomyxoviridae - physiology ; Orthomyxoviridae Infections - immunology ; Orthomyxoviridae Infections - prevention & control ; Orthomyxoviridae Infections - virology ; Pandemics ; Proteins ; Reading ; Replication ; Respiratory System - drug effects ; Respiratory System - immunology ; Respiratory System - virology ; Rodents ; Strains (organisms) ; Surfactants ; Swine flu ; Vaccines ; Virulence ; Virulence (Microbiology) ; Virulence - drug effects ; Virus Replication - drug effects ; Viruses</subject><ispartof>PloS one, 2011-03, Vol.6 (3), p.e17618-e17618</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Tate et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1(+) cells, not neutrophils, limit disease severity during mild influenza infections.</description><subject>Administration, Intranasal</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Avian flu</subject><subject>Biology</subject><subject>Bone marrow</subject><subject>Disease control</subject><subject>Granulocytes</subject><subject>Health aspects</subject><subject>Immunology</subject><subject>Infections</subject><subject>Influenza</subject><subject>Influenza virus</subject><subject>Influenza viruses</subject><subject>Lectins</subject><subject>Leukocytes</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lungs</subject><subject>Medical treatment</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neutropenia</subject><subject>Neutrophil Infiltration - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tate, Michelle D</au><au>Ioannidis, Lisa J</au><au>Croker, Ben</au><au>Brown, Lorena E</au><au>Brooks, Andrew G</au><au>Reading, Patrick C</au><au>Fouchier, Ron</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of neutrophils during mild and severe influenza virus infections of mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-03-14</date><risdate>2011</risdate><volume>6</volume><issue>3</issue><spage>e17618</spage><epage>e17618</epage><pages>e17618-e17618</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Neutrophils have been implicated in both protective and pathological responses following influenza virus infections. We have used mAb 1A8 (anti-Ly6G) to specifically deplete LyG6(high) neutrophils and induce neutropenia in mice infected with virus strains known to differ in virulence. Mice were also treated with mAb RB6-8C5 (anti-Ly6C/G or anti-Gr-1), a mAb widely used to investigate the role of neutrophils in mice that has been shown to bind and deplete additional leukocyte subsets. Using mAb 1A8, we confirm the beneficial role of neutrophils in mice infected with virus strains of intermediate (HKx31; H3N2) or high (PR8; H1N1) virulence whereas treatment of mice infected with an avirulent strain (BJx109; H3N2) did not affect disease or virus replication. Treatment of BJx109-infected mice with mAb RB6-8C5 was, however, associated with significant weight loss and enhanced virus replication indicating that other Gr-1(+) cells, not neutrophils, limit disease severity during mild influenza infections.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21423798</pmid><doi>10.1371/journal.pone.0017618</doi><tpages>e17618</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Administration, Intranasal Animals Antibodies, Monoclonal - pharmacology Avian flu Biology Bone marrow Disease control Granulocytes Health aspects Immunology Infections Influenza Influenza virus Influenza viruses Lectins Leukocytes Leukocytes (neutrophilic) Lungs Medical treatment Medicine Mice Mice, Inbred C57BL Neutropenia Neutrophil Infiltration - drug effects Neutrophils Neutrophils - drug effects Neutrophils - immunology Neutrophils - virology Orthomyxoviridae - drug effects Orthomyxoviridae - pathogenicity Orthomyxoviridae - physiology Orthomyxoviridae Infections - immunology Orthomyxoviridae Infections - prevention & control Orthomyxoviridae Infections - virology Pandemics Proteins Reading Replication Respiratory System - drug effects Respiratory System - immunology Respiratory System - virology Rodents Strains (organisms) Surfactants Swine flu Vaccines Virulence Virulence (Microbiology) Virulence - drug effects Virus Replication - drug effects Viruses |
| title | The role of neutrophils during mild and severe influenza virus infections of mice |
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