Multiparameter RNA and codon optimization: a standardized tool to assess and enhance autologous mammalian gene expression

Autologous expression of recombinant human proteins in human cells for biomedical research and product development is often hampered by low expression yields limiting subsequent structural and functional analyses. Following RNA and codon optimization, 50 candidate genes representing five classes of...

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Veröffentlicht in:	PloS one 2011-03, Vol.6 (3), p.e17596-e17596
Hauptverfasser:	Fath, Stephan, Bauer, Asli Petra, Liss, Michael, Spriestersbach, Anne, Maertens, Barbara, Hahn, Peter, Ludwig, Christine, Schäfer, Frank, Graf, Marcus, Wagner, Ralf
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptation Animals Base Sequence Biology Cdc2 protein Cells (Biology) CHO Cells Codon - genetics Codons Cricetinae Cricetulus Design DNA binding proteins E coli Gene expression Gene Expression Regulation Gene Knockdown Techniques Gene therapy Genes Genes, Synthetic Genetic Techniques - standards Genomics Gram-positive bacteria HEK293 Cells Human papillomavirus Humans Immunization Immunomodulation Immunomodulators Insect cells Insects JNK Mitogen-Activated Protein Kinases - metabolism JNK protein JNK3 protein Kinases Leukemia Mammals Mammals - genetics Mathematical models Membrane proteins Messenger RNA mRNA stability Optimization Polymerase Product development Protein expression Protein kinase Protein kinases Proteins Reference Standards Ribonucleic acid RNA RNA - genetics RNA Interference siRNA Solubility Structure-function relationships Transcription (Genetics) Transcription factors
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