A three-component gene expression system and its application for inducible flavonoid overproduction in transgenic Arabidopsis thaliana
Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin,...
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| Veröffentlicht in: | PloS one 2011-03, Vol.6 (3), p.e17603-e17603 |
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| creator | Feng, Yue Cao, Cong-Mei Vikram, Meenu Park, Sunghun Kim, Hye Jin Hong, Jong Chan Cisneros-Zevallos, Luis Koiwa, Hisashi |
| description | Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A) promoter, CBF3 (C-repeat Binding Factor 3) transcription factor and cpl1-2 (CTD phosphatase-like 1) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of Anthocyanin Pigment 1) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone. |
| doi_str_mv | 10.1371/journal.pone.0017603 |
| format | Article |
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However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A) promoter, CBF3 (C-repeat Binding Factor 3) transcription factor and cpl1-2 (CTD phosphatase-like 1) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of Anthocyanin Pigment 1) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0017603</identifier><identifier>PMID: 21408135</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abiotic stress ; Agriculture ; Anthocyanins ; Anthocyanins - biosynthesis ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Binding sites ; Biology ; Biosynthesis ; Biosynthetic Pathways - genetics ; Biotechnology ; Chromatography, High Pressure Liquid ; Cloning ; Cold Temperature ; Cold treatment ; CTD phosphatase ; Dehydration ; Engineering ; Environmental engineering ; Feedforward ; Flavonoids ; Flowers & plants ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genes ; Genetic research ; Genetic Techniques ; Genetically modified plants ; Growth rate ; Homozygote ; Horticulture ; Host plants ; Kaempferol ; Life sciences ; Mass Spectrometry ; Metabolic engineering ; Metabolism ; Molecular biology ; Mutation ; Osmotic Pressure ; Osmotic stress ; Pancreatitis-Associated Proteins ; Phosphatase ; Phytochemicals ; Pigments ; Plants (botany) ; Plants, Genetically Modified ; Promoters ; Repressors ; Reverse Transcriptase Polymerase Chain Reaction ; Signal transduction ; Stress ; Stress, Physiological - genetics ; Stresses ; Time Factors ; Transcription factors ; Transcription Factors - metabolism ; Transgenes ; Transgenes - genetics ; Transgenic plants ; Vectors (Biology)</subject><ispartof>PloS one, 2011-03, Vol.6 (3), p.e17603-e17603</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Feng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Feng et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c723t-f3ad1639dbff29832573f40becf2a27cc6e34a84fa787a2e65d70c806c5a0f383</citedby><cites>FETCH-LOGICAL-c723t-f3ad1639dbff29832573f40becf2a27cc6e34a84fa787a2e65d70c806c5a0f383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050924/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050924/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21408135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Yue</creatorcontrib><creatorcontrib>Cao, Cong-Mei</creatorcontrib><creatorcontrib>Vikram, Meenu</creatorcontrib><creatorcontrib>Park, Sunghun</creatorcontrib><creatorcontrib>Kim, Hye Jin</creatorcontrib><creatorcontrib>Hong, Jong Chan</creatorcontrib><creatorcontrib>Cisneros-Zevallos, Luis</creatorcontrib><creatorcontrib>Koiwa, Hisashi</creatorcontrib><title>A three-component gene expression system and its application for inducible flavonoid overproduction in transgenic Arabidopsis thaliana</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A) promoter, CBF3 (C-repeat Binding Factor 3) transcription factor and cpl1-2 (CTD phosphatase-like 1) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of Anthocyanin Pigment 1) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone.</description><subject>Abiotic stress</subject><subject>Agriculture</subject><subject>Anthocyanins</subject><subject>Anthocyanins - biosynthesis</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Binding sites</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Biosynthetic Pathways - genetics</subject><subject>Biotechnology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cloning</subject><subject>Cold Temperature</subject><subject>Cold treatment</subject><subject>CTD phosphatase</subject><subject>Dehydration</subject><subject>Engineering</subject><subject>Environmental engineering</subject><subject>Feedforward</subject><subject>Flavonoids</subject><subject>Flowers & plants</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genetic Techniques</subject><subject>Genetically modified plants</subject><subject>Growth rate</subject><subject>Homozygote</subject><subject>Horticulture</subject><subject>Host plants</subject><subject>Kaempferol</subject><subject>Life sciences</subject><subject>Mass Spectrometry</subject><subject>Metabolic engineering</subject><subject>Metabolism</subject><subject>Molecular biology</subject><subject>Mutation</subject><subject>Osmotic Pressure</subject><subject>Osmotic stress</subject><subject>Pancreatitis-Associated Proteins</subject><subject>Phosphatase</subject><subject>Phytochemicals</subject><subject>Pigments</subject><subject>Plants (botany)</subject><subject>Plants, Genetically Modified</subject><subject>Promoters</subject><subject>Repressors</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Signal transduction</subject><subject>Stress</subject><subject>Stress, Physiological - genetics</subject><subject>Stresses</subject><subject>Time Factors</subject><subject>Transcription factors</subject><subject>Transcription Factors - metabolism</subject><subject>Transgenes</subject><subject>Transgenes - genetics</subject><subject>Transgenic plants</subject><subject>Vectors (Biology)</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQ6GlF7vVwbbkm8ISeggEAj3dill5tKuglRzJDskL9LkrZzchWwItupCY-eYfaUZTFC8pmVMu6IfzMEYPbt4Hj3NCqGgIf1Qc0pazWcMIf3zvfFA8S-mckJrLpnlaHDBaEUl5fVj8XpTDOiLOdNhMSn4oV-ixxKs-Yko2-DJdpwE3JfiutEMqoe-d1TBMLhNiaX03art0WBoHl8EH25XhEmMfQ3bcYNaXQwSfsrLV5SLC0nahTzbl3OAseHhePDHgEr7Y7UfFz8-ffhx_nZ2efTk5XpzOtGB8mBkOHW142y2NYa3krBbcVGSJ2jBgQusGeQWyMiCkAIZN3QmiJWl0DcRwyY-K11vd3oWkdiVMirK2roSQhGfiZEt0Ac5VH-0G4rUKYNWNIcSVgjhY7VDVoqbLjtddK2XVagaSYk0ZR2yxYqTJWh932cblBjudqxvB7Ynue7xdq1W4VJzUpGVVFni3E4jhYsQ0qI1NGp0Dj2FMSuYGN1Mz_03WopJ1RWgm3_xFPlyGHbWC_FLrTcgX1JOmWlSiaTPSTNT8ASqvDjdW5-9kbLbvBbzfC8jMgFfDCsaU1Mn3b__Pnv3aZ9_eY9cIblin4Mbp-6V9sNqCOoaUIpq7blCiprm6rYaapkHt5iqHvbrfybug20HifwAddR-i</recordid><startdate>20110308</startdate><enddate>20110308</enddate><creator>Feng, Yue</creator><creator>Cao, Cong-Mei</creator><creator>Vikram, Meenu</creator><creator>Park, Sunghun</creator><creator>Kim, Hye Jin</creator><creator>Hong, Jong Chan</creator><creator>Cisneros-Zevallos, Luis</creator><creator>Koiwa, Hisashi</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110308</creationdate><title>A three-component gene expression system and its application for inducible flavonoid overproduction in transgenic Arabidopsis thaliana</title><author>Feng, Yue ; Cao, Cong-Mei ; Vikram, Meenu ; Park, Sunghun ; Kim, Hye Jin ; Hong, Jong Chan ; Cisneros-Zevallos, Luis ; Koiwa, Hisashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c723t-f3ad1639dbff29832573f40becf2a27cc6e34a84fa787a2e65d70c806c5a0f383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abiotic stress</topic><topic>Agriculture</topic><topic>Anthocyanins</topic><topic>Anthocyanins - biosynthesis</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Binding sites</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Biosynthetic Pathways - genetics</topic><topic>Biotechnology</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cloning</topic><topic>Cold Temperature</topic><topic>Cold treatment</topic><topic>CTD phosphatase</topic><topic>Dehydration</topic><topic>Engineering</topic><topic>Environmental engineering</topic><topic>Feedforward</topic><topic>Flavonoids</topic><topic>Flowers & plants</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Genetic Techniques</topic><topic>Genetically modified plants</topic><topic>Growth rate</topic><topic>Homozygote</topic><topic>Horticulture</topic><topic>Host plants</topic><topic>Kaempferol</topic><topic>Life sciences</topic><topic>Mass Spectrometry</topic><topic>Metabolic engineering</topic><topic>Metabolism</topic><topic>Molecular biology</topic><topic>Mutation</topic><topic>Osmotic Pressure</topic><topic>Osmotic stress</topic><topic>Pancreatitis-Associated Proteins</topic><topic>Phosphatase</topic><topic>Phytochemicals</topic><topic>Pigments</topic><topic>Plants (botany)</topic><topic>Plants, Genetically Modified</topic><topic>Promoters</topic><topic>Repressors</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Signal transduction</topic><topic>Stress</topic><topic>Stress, Physiological - genetics</topic><topic>Stresses</topic><topic>Time Factors</topic><topic>Transcription factors</topic><topic>Transcription Factors - metabolism</topic><topic>Transgenes</topic><topic>Transgenes - genetics</topic><topic>Transgenic plants</topic><topic>Vectors (Biology)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Yue</creatorcontrib><creatorcontrib>Cao, Cong-Mei</creatorcontrib><creatorcontrib>Vikram, Meenu</creatorcontrib><creatorcontrib>Park, Sunghun</creatorcontrib><creatorcontrib>Kim, Hye Jin</creatorcontrib><creatorcontrib>Hong, Jong Chan</creatorcontrib><creatorcontrib>Cisneros-Zevallos, Luis</creatorcontrib><creatorcontrib>Koiwa, Hisashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Yue</au><au>Cao, Cong-Mei</au><au>Vikram, Meenu</au><au>Park, Sunghun</au><au>Kim, Hye Jin</au><au>Hong, Jong Chan</au><au>Cisneros-Zevallos, Luis</au><au>Koiwa, Hisashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A three-component gene expression system and its application for inducible flavonoid overproduction in transgenic Arabidopsis thaliana</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-03-08</date><risdate>2011</risdate><volume>6</volume><issue>3</issue><spage>e17603</spage><epage>e17603</epage><pages>e17603-e17603</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Inducible gene expression is a powerful tool to study and engineer genes whose overexpression could be detrimental for the host organisms. However, only limited systems have been adopted in plant biotechnology. We have developed an osmotically inducible system using three components of plant origin, RD29a (Responsive to Dehydration 29A) promoter, CBF3 (C-repeat Binding Factor 3) transcription factor and cpl1-2 (CTD phosphatase-like 1) mutation. The osmotic stress responsible RD29a promoter contains the CBF3 binding sites and thus RD29A-CBF3 feedforward cassette enhances induction of RD29a promoter under stress. The cpl1-2 mutation in a host repressor CPL1 promotes stress responsible RD29a promoter expression. The efficacy of this system was tested using PAP1 (Production of Anthocyanin Pigment 1) transgene, a model transcription factor that regulates the anthocyanin pathway in Arabidopsis. While transgenic plants with only one or two of three components did not reproducibly accumulate anthocyanin pigments above the control level, transgenic cpl1 plants containing homozygous RD29a-PAP1 and RD29a-CBF3 transgenes produced 30-fold higher level of total anthocyanins than control plants upon cold treatment. Growth retardation and phytochemical production of transgenic plants were minimum under normal conditions. The flavonoid profile in cold-induced transgenic plants was determined by LC/MS/MS, which resembled that of previously reported pap1-D plants but enriched for kaempferol derivatives. These results establish the functionality of the inducible three-component gene expression system in plant metabolic engineering. Furthermore, we show that PAP1 and environmental signals synergistically regulate the flavonoid pathway to produce a unique flavonoid blend that has not been produced by PAP1 overexpression or cold treatment alone.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21408135</pmid><doi>10.1371/journal.pone.0017603</doi><tpages>e17603</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1295477803 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abiotic stress Agriculture Anthocyanins Anthocyanins - biosynthesis Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Binding sites Biology Biosynthesis Biosynthetic Pathways - genetics Biotechnology Chromatography, High Pressure Liquid Cloning Cold Temperature Cold treatment CTD phosphatase Dehydration Engineering Environmental engineering Feedforward Flavonoids Flowers & plants Gene expression Gene Expression Profiling Gene Expression Regulation, Plant Genes Genetic research Genetic Techniques Genetically modified plants Growth rate Homozygote Horticulture Host plants Kaempferol Life sciences Mass Spectrometry Metabolic engineering Metabolism Molecular biology Mutation Osmotic Pressure Osmotic stress Pancreatitis-Associated Proteins Phosphatase Phytochemicals Pigments Plants (botany) Plants, Genetically Modified Promoters Repressors Reverse Transcriptase Polymerase Chain Reaction Signal transduction Stress Stress, Physiological - genetics Stresses Time Factors Transcription factors Transcription Factors - metabolism Transgenes Transgenes - genetics Transgenic plants Vectors (Biology) |
| title | A three-component gene expression system and its application for inducible flavonoid overproduction in transgenic Arabidopsis thaliana |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T11%3A08%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20three-component%20gene%20expression%20system%20and%20its%20application%20for%20inducible%20flavonoid%20overproduction%20in%20transgenic%20Arabidopsis%20thaliana&rft.jtitle=PloS%20one&rft.au=Feng,%20Yue&rft.date=2011-03-08&rft.volume=6&rft.issue=3&rft.spage=e17603&rft.epage=e17603&rft.pages=e17603-e17603&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0017603&rft_dat=%3Cgale_plos_%3EA476903363%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1295477803&rft_id=info:pmid/21408135&rft_galeid=A476903363&rft_doaj_id=oai_doaj_org_article_5751bd35d98849c2a81e5123ee9e4206&rfr_iscdi=true |