A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes
Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of...
Gespeichert in:
| Veröffentlicht in: | PloS one 2010-11, Vol.5 (11), p.e15499-e15499 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e15499 |
|---|---|
| container_issue | 11 |
| container_start_page | e15499 |
| container_title | PloS one |
| container_volume | 5 |
| creator | Scherrer, Tanja Mittal, Nitish Janga, Sarath Chandra Gerber, André P |
| description | Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of RNA from budding yeast Saccharomyces cerevisiae. Surprisingly, more than half of these proteins represent previously known enzymes, many of them acting in metabolism, providing opportunities to directly connect intermediary metabolism with posttranscriptional gene regulation. We mapped the RNA targets for 13 proteins identified in this screen and found that they were associated with distinct groups of mRNAs, some of them coding for functionally related proteins. We also found that overexpression of the enzyme Map1 negatively affects the expression of experimentally defined mRNA targets. Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation. |
| doi_str_mv | 10.1371/journal.pone.0015499 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1295223348</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A473834789</galeid><doaj_id>oai_doaj_org_article_b43cf67b0f934c36a7723c4744bb4ed1</doaj_id><sourcerecordid>A473834789</sourcerecordid><originalsourceid>FETCH-LOGICAL-c723t-d27ecfcec25561cdeb0621a7c290c53d2ea7d091132b3d4003295c8444002fc53</originalsourceid><addsrcrecordid>eNqNk11rFDEUhgdRbK3-A9EBQfFi13zNR26EpfixUCzU6m3IJGd2s8wka5IR119vtjstO9KLkouEnOd9k5yTk2UvMZpjWuEPGzd4K7v51lmYI4QLxvmj7BRzSmYlQfTx0fokexbCBqGC1mX5NDshGBPGUXWaXS_yoDyAzVvn86tvi1ljrDZ2lW-9i2BsyI3NdyBDzPcBJSOEXA-yy9vBqmhcIvbSXtpdDvbvrofwPHvSyi7Ai3E-y358_nR9_nV2cflleb64mKmK0DjTpALVKlCkKEqsNDSoJFhWinCkCqoJyEojjjElDdUMIUp4oWrG0pK0iTjLXh98t50LYkxIEDhhhFDK6kQsD4R2ciO23vTS74STRtxsOL8S0kejOhANo6otqwa1nDJFS1mlSypWMdY0DDROXh_H04amB63ARi-7iek0Ys1arNxvQXhd15gmg3ejgXe_BghR9CYo6DppwQ1B1CXjBSXlA0hc8BKVBUvkm__I-9MwUiuZXmps69IF1d5TLFhFa8qqmidqfg-VhobeqPTNWpP2J4L3E0FiIvyJKzmEIJbfrx7OXv6csm-P2DXILq6D64ab3zYF2QFU3oXgob2rBkZi3yW32RD7LhFjlyTZq-NK3olu24L-A81pC2A</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1295223348</pqid></control><display><type>article</type><title>A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Scherrer, Tanja ; Mittal, Nitish ; Janga, Sarath Chandra ; Gerber, André P</creator><creatorcontrib>Scherrer, Tanja ; Mittal, Nitish ; Janga, Sarath Chandra ; Gerber, André P</creatorcontrib><description>Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of RNA from budding yeast Saccharomyces cerevisiae. Surprisingly, more than half of these proteins represent previously known enzymes, many of them acting in metabolism, providing opportunities to directly connect intermediary metabolism with posttranscriptional gene regulation. We mapped the RNA targets for 13 proteins identified in this screen and found that they were associated with distinct groups of mRNAs, some of them coding for functionally related proteins. We also found that overexpression of the enzyme Map1 negatively affects the expression of experimentally defined mRNA targets. Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0015499</identifier><identifier>PMID: 21124907</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aminopeptidases - genetics ; Aminopeptidases - metabolism ; Baking yeast ; Binding ; Binding proteins ; Bioinformatics ; Biology ; DNA microarrays ; Enzymes ; Enzymes - genetics ; Enzymes - metabolism ; Gene expression ; Gene Expression Profiling ; Gene regulation ; Genes ; Genetic aspects ; Laboratories ; Localization ; Medical research ; Messenger RNA ; Metabolism ; Methionyl Aminopeptidases ; Molecular biology ; Molecular weight ; Oligonucleotide Array Sequence Analysis ; Ontology ; Open source software ; Pharmaceutical sciences ; Physiological aspects ; Post-transcription ; Protein Array Analysis ; Protein arrays ; Protein Binding ; Proteins ; Proteome - genetics ; Proteome - metabolism ; Proteomics ; Ribonucleic acid ; RNA ; RNA, Fungal - genetics ; RNA, Fungal - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA-binding protein ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Studies ; Target recognition ; Transcription ; Transcription (Genetics) ; Trends ; Yeast</subject><ispartof>PloS one, 2010-11, Vol.5 (11), p.e15499-e15499</ispartof><rights>COPYRIGHT 2010 Public Library of Science</rights><rights>2010 Scherrer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Scherrer et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c723t-d27ecfcec25561cdeb0621a7c290c53d2ea7d091132b3d4003295c8444002fc53</citedby><cites>FETCH-LOGICAL-c723t-d27ecfcec25561cdeb0621a7c290c53d2ea7d091132b3d4003295c8444002fc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988813/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988813/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21124907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scherrer, Tanja</creatorcontrib><creatorcontrib>Mittal, Nitish</creatorcontrib><creatorcontrib>Janga, Sarath Chandra</creatorcontrib><creatorcontrib>Gerber, André P</creatorcontrib><title>A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of RNA from budding yeast Saccharomyces cerevisiae. Surprisingly, more than half of these proteins represent previously known enzymes, many of them acting in metabolism, providing opportunities to directly connect intermediary metabolism with posttranscriptional gene regulation. We mapped the RNA targets for 13 proteins identified in this screen and found that they were associated with distinct groups of mRNAs, some of them coding for functionally related proteins. We also found that overexpression of the enzyme Map1 negatively affects the expression of experimentally defined mRNA targets. Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation.</description><subject>Aminopeptidases - genetics</subject><subject>Aminopeptidases - metabolism</subject><subject>Baking yeast</subject><subject>Binding</subject><subject>Binding proteins</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>DNA microarrays</subject><subject>Enzymes</subject><subject>Enzymes - genetics</subject><subject>Enzymes - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Laboratories</subject><subject>Localization</subject><subject>Medical research</subject><subject>Messenger RNA</subject><subject>Metabolism</subject><subject>Methionyl Aminopeptidases</subject><subject>Molecular biology</subject><subject>Molecular weight</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Ontology</subject><subject>Open source software</subject><subject>Pharmaceutical sciences</subject><subject>Physiological aspects</subject><subject>Post-transcription</subject><subject>Protein Array Analysis</subject><subject>Protein arrays</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Proteome - genetics</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Fungal - genetics</subject><subject>RNA, Fungal - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-binding protein</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Studies</subject><subject>Target recognition</subject><subject>Transcription</subject><subject>Transcription (Genetics)</subject><subject>Trends</subject><subject>Yeast</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9EBQfFi13zNR26EpfixUCzU6m3IJGd2s8wka5IR119vtjstO9KLkouEnOd9k5yTk2UvMZpjWuEPGzd4K7v51lmYI4QLxvmj7BRzSmYlQfTx0fokexbCBqGC1mX5NDshGBPGUXWaXS_yoDyAzVvn86tvi1ljrDZ2lW-9i2BsyI3NdyBDzPcBJSOEXA-yy9vBqmhcIvbSXtpdDvbvrofwPHvSyi7Ai3E-y358_nR9_nV2cflleb64mKmK0DjTpALVKlCkKEqsNDSoJFhWinCkCqoJyEojjjElDdUMIUp4oWrG0pK0iTjLXh98t50LYkxIEDhhhFDK6kQsD4R2ciO23vTS74STRtxsOL8S0kejOhANo6otqwa1nDJFS1mlSypWMdY0DDROXh_H04amB63ARi-7iek0Ys1arNxvQXhd15gmg3ejgXe_BghR9CYo6DppwQ1B1CXjBSXlA0hc8BKVBUvkm__I-9MwUiuZXmps69IF1d5TLFhFa8qqmidqfg-VhobeqPTNWpP2J4L3E0FiIvyJKzmEIJbfrx7OXv6csm-P2DXILq6D64ab3zYF2QFU3oXgob2rBkZi3yW32RD7LhFjlyTZq-NK3olu24L-A81pC2A</recordid><startdate>20101111</startdate><enddate>20101111</enddate><creator>Scherrer, Tanja</creator><creator>Mittal, Nitish</creator><creator>Janga, Sarath Chandra</creator><creator>Gerber, André P</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20101111</creationdate><title>A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes</title><author>Scherrer, Tanja ; Mittal, Nitish ; Janga, Sarath Chandra ; Gerber, André P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c723t-d27ecfcec25561cdeb0621a7c290c53d2ea7d091132b3d4003295c8444002fc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aminopeptidases - genetics</topic><topic>Aminopeptidases - metabolism</topic><topic>Baking yeast</topic><topic>Binding</topic><topic>Binding proteins</topic><topic>Bioinformatics</topic><topic>Biology</topic><topic>DNA microarrays</topic><topic>Enzymes</topic><topic>Enzymes - genetics</topic><topic>Enzymes - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Laboratories</topic><topic>Localization</topic><topic>Medical research</topic><topic>Messenger RNA</topic><topic>Metabolism</topic><topic>Methionyl Aminopeptidases</topic><topic>Molecular biology</topic><topic>Molecular weight</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Ontology</topic><topic>Open source software</topic><topic>Pharmaceutical sciences</topic><topic>Physiological aspects</topic><topic>Post-transcription</topic><topic>Protein Array Analysis</topic><topic>Protein arrays</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Proteome - genetics</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Fungal - genetics</topic><topic>RNA, Fungal - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-binding protein</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Studies</topic><topic>Target recognition</topic><topic>Transcription</topic><topic>Transcription (Genetics)</topic><topic>Trends</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scherrer, Tanja</creatorcontrib><creatorcontrib>Mittal, Nitish</creatorcontrib><creatorcontrib>Janga, Sarath Chandra</creatorcontrib><creatorcontrib>Gerber, André P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scherrer, Tanja</au><au>Mittal, Nitish</au><au>Janga, Sarath Chandra</au><au>Gerber, André P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2010-11-11</date><risdate>2010</risdate><volume>5</volume><issue>11</issue><spage>e15499</spage><epage>e15499</epage><pages>e15499-e15499</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of RNA from budding yeast Saccharomyces cerevisiae. Surprisingly, more than half of these proteins represent previously known enzymes, many of them acting in metabolism, providing opportunities to directly connect intermediary metabolism with posttranscriptional gene regulation. We mapped the RNA targets for 13 proteins identified in this screen and found that they were associated with distinct groups of mRNAs, some of them coding for functionally related proteins. We also found that overexpression of the enzyme Map1 negatively affects the expression of experimentally defined mRNA targets. Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21124907</pmid><doi>10.1371/journal.pone.0015499</doi><tpages>e15499</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2010-11, Vol.5 (11), p.e15499-e15499 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1295223348 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Aminopeptidases - genetics Aminopeptidases - metabolism Baking yeast Binding Binding proteins Bioinformatics Biology DNA microarrays Enzymes Enzymes - genetics Enzymes - metabolism Gene expression Gene Expression Profiling Gene regulation Genes Genetic aspects Laboratories Localization Medical research Messenger RNA Metabolism Methionyl Aminopeptidases Molecular biology Molecular weight Oligonucleotide Array Sequence Analysis Ontology Open source software Pharmaceutical sciences Physiological aspects Post-transcription Protein Array Analysis Protein arrays Protein Binding Proteins Proteome - genetics Proteome - metabolism Proteomics Ribonucleic acid RNA RNA, Fungal - genetics RNA, Fungal - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism RNA-binding protein RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Studies Target recognition Transcription Transcription (Genetics) Trends Yeast |
| title | A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T19%3A03%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20screen%20for%20RNA-binding%20proteins%20in%20yeast%20indicates%20dual%20functions%20for%20many%20enzymes&rft.jtitle=PloS%20one&rft.au=Scherrer,%20Tanja&rft.date=2010-11-11&rft.volume=5&rft.issue=11&rft.spage=e15499&rft.epage=e15499&rft.pages=e15499-e15499&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0015499&rft_dat=%3Cgale_plos_%3EA473834789%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1295223348&rft_id=info:pmid/21124907&rft_galeid=A473834789&rft_doaj_id=oai_doaj_org_article_b43cf67b0f934c36a7723c4744bb4ed1&rfr_iscdi=true |