Acellular pertussis booster in adolescents induces Th1 and memory CD8+ T cell immune response
In a number of countries, whole cell pertussis vaccines (wcP) were replaced by acellular vaccines (aP) due to an improved reactogenicity profile. Pertussis immunization leads to specific antibody production with the help of CD4(+) T cells. In earlier studies in infants and young children, wcP vaccin...
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| Veröffentlicht in: | PloS one 2011-03, Vol.6 (3), p.e17271 |
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| description | In a number of countries, whole cell pertussis vaccines (wcP) were replaced by acellular vaccines (aP) due to an improved reactogenicity profile. Pertussis immunization leads to specific antibody production with the help of CD4(+) T cells. In earlier studies in infants and young children, wcP vaccines selectively induced a Th1 dominated immune response, whereas aP vaccines led to a Th2 biased response. To obtain data on Th1 or Th2 dominance of the immune response in adolescents receiving an aP booster immunization after a wcP or aP primary immunization, we analyzed the concentration of Th1 (IL-2, TNF-α, INF-γ) and Th2 (IL-4, IL-5, IL-10) cytokines in supernatants of lymphocyte cultures specifically stimulated with pertussis antigens. We also investigated the presence of cytotoxic T cell responses against the facultative intracellular bacterium Bordetella pertussis by quantifying pertussis-specific CD8(+) T cell activation following the aP booster immunization. Here we show that the adolescent aP booster vaccination predominantly leads to a Th1 immune response based on IFNgamma secretion upon stimulation with pertussis antigen, irrespective of a prior whole cell or acellular primary vaccination. The vaccination also induces an increase in peripheral CD8(+)CD69(+) activated pertussis-specific memory T cells four weeks after vaccination. The Th1 bias of this immune response could play a role for the decreased local reactogenicity of this adolescent aP booster immunization when compared to the preceding childhood acellular pertussis booster. Pertussis-specific CD8(+) memory T cells may contribute to protection against clinical pertussis. |
| doi_str_mv | 10.1371/journal.pone.0017271 |
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Pertussis immunization leads to specific antibody production with the help of CD4(+) T cells. In earlier studies in infants and young children, wcP vaccines selectively induced a Th1 dominated immune response, whereas aP vaccines led to a Th2 biased response. To obtain data on Th1 or Th2 dominance of the immune response in adolescents receiving an aP booster immunization after a wcP or aP primary immunization, we analyzed the concentration of Th1 (IL-2, TNF-α, INF-γ) and Th2 (IL-4, IL-5, IL-10) cytokines in supernatants of lymphocyte cultures specifically stimulated with pertussis antigens. We also investigated the presence of cytotoxic T cell responses against the facultative intracellular bacterium Bordetella pertussis by quantifying pertussis-specific CD8(+) T cell activation following the aP booster immunization. Here we show that the adolescent aP booster vaccination predominantly leads to a Th1 immune response based on IFNgamma secretion upon stimulation with pertussis antigen, irrespective of a prior whole cell or acellular primary vaccination. The vaccination also induces an increase in peripheral CD8(+)CD69(+) activated pertussis-specific memory T cells four weeks after vaccination. The Th1 bias of this immune response could play a role for the decreased local reactogenicity of this adolescent aP booster immunization when compared to the preceding childhood acellular pertussis booster. Pertussis-specific CD8(+) memory T cells may contribute to protection against clinical pertussis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0017271</identifier><identifier>PMID: 21408149</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adolescents ; Age ; Aluminum ; Antigens ; Antigens, CD - immunology ; Antigens, Differentiation, T-Lymphocyte - immunology ; Biology ; Bordetella ; Bordetella pertussis ; CD4 antigen ; CD69 antigen ; CD8 antigen ; CD8-Positive T-Lymphocytes - immunology ; Cell activation ; Chicken pox ; Child ; Children ; Children & youth ; Clinical trials ; Cytokines ; Cytokines - immunology ; Cytotoxicity ; Demography ; Diphtheria ; Female ; Humans ; Immune response ; Immune response (cell-mediated) ; Immune system ; Immunization ; Immunization, Secondary ; Immunologic Memory - immunology ; Immunological memory ; Immunophenotyping ; Immunotherapy ; Infants ; Infections ; Interleukin 10 ; Interleukin 2 ; Interleukin 4 ; Interleukin 5 ; Lectins, C-Type - immunology ; Licensed products ; Localization ; Lymphocyte Activation - immunology ; Lymphocytes ; Lymphocytes T ; Male ; Medicine ; Memory cells ; Pertussis ; Pertussis Vaccine - immunology ; Studies ; Teenagers ; Tetanus ; Th1 Cells - immunology ; Th2 Cells - immunology ; Tumor necrosis factor-α ; Vaccination ; Vaccines ; Vaccines, Acellular - immunology ; Whooping cough ; Whooping Cough - immunology</subject><ispartof>PloS one, 2011-03, Vol.6 (3), p.e17271</ispartof><rights>2011 Rieber et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Rieber et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-9a611e1621d5ce767a31b23884f1383626b178d5f5dc9ca4ba313c763d3d5d2f3</citedby><cites>FETCH-LOGICAL-c557t-9a611e1621d5ce767a31b23884f1383626b178d5f5dc9ca4ba313c763d3d5d2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050840/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3050840/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21408149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rieber, Nikolaus</creatorcontrib><creatorcontrib>Graf, Anna</creatorcontrib><creatorcontrib>Hartl, Dominik</creatorcontrib><creatorcontrib>Urschel, Simon</creatorcontrib><creatorcontrib>Belohradsky, Bernd H</creatorcontrib><creatorcontrib>Liese, Johannes</creatorcontrib><title>Acellular pertussis booster in adolescents induces Th1 and memory CD8+ T cell immune response</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In a number of countries, whole cell pertussis vaccines (wcP) were replaced by acellular vaccines (aP) due to an improved reactogenicity profile. Pertussis immunization leads to specific antibody production with the help of CD4(+) T cells. In earlier studies in infants and young children, wcP vaccines selectively induced a Th1 dominated immune response, whereas aP vaccines led to a Th2 biased response. To obtain data on Th1 or Th2 dominance of the immune response in adolescents receiving an aP booster immunization after a wcP or aP primary immunization, we analyzed the concentration of Th1 (IL-2, TNF-α, INF-γ) and Th2 (IL-4, IL-5, IL-10) cytokines in supernatants of lymphocyte cultures specifically stimulated with pertussis antigens. We also investigated the presence of cytotoxic T cell responses against the facultative intracellular bacterium Bordetella pertussis by quantifying pertussis-specific CD8(+) T cell activation following the aP booster immunization. Here we show that the adolescent aP booster vaccination predominantly leads to a Th1 immune response based on IFNgamma secretion upon stimulation with pertussis antigen, irrespective of a prior whole cell or acellular primary vaccination. The vaccination also induces an increase in peripheral CD8(+)CD69(+) activated pertussis-specific memory T cells four weeks after vaccination. The Th1 bias of this immune response could play a role for the decreased local reactogenicity of this adolescent aP booster immunization when compared to the preceding childhood acellular pertussis booster. 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immunology</subject><subject>Immunological memory</subject><subject>Immunophenotyping</subject><subject>Immunotherapy</subject><subject>Infants</subject><subject>Infections</subject><subject>Interleukin 10</subject><subject>Interleukin 2</subject><subject>Interleukin 4</subject><subject>Interleukin 5</subject><subject>Lectins, C-Type - immunology</subject><subject>Licensed products</subject><subject>Localization</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Medicine</subject><subject>Memory cells</subject><subject>Pertussis</subject><subject>Pertussis Vaccine - immunology</subject><subject>Studies</subject><subject>Teenagers</subject><subject>Tetanus</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - immunology</subject><subject>Tumor necrosis factor-α</subject><subject>Vaccination</subject><subject>Vaccines</subject><subject>Vaccines, Acellular - immunology</subject><subject>Whooping cough</subject><subject>Whooping Cough - 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immunology</topic><topic>Antigens, Differentiation, T-Lymphocyte - immunology</topic><topic>Biology</topic><topic>Bordetella</topic><topic>Bordetella pertussis</topic><topic>CD4 antigen</topic><topic>CD69 antigen</topic><topic>CD8 antigen</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cell activation</topic><topic>Chicken pox</topic><topic>Child</topic><topic>Children</topic><topic>Children & youth</topic><topic>Clinical trials</topic><topic>Cytokines</topic><topic>Cytokines - immunology</topic><topic>Cytotoxicity</topic><topic>Demography</topic><topic>Diphtheria</topic><topic>Female</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune response (cell-mediated)</topic><topic>Immune system</topic><topic>Immunization</topic><topic>Immunization, Secondary</topic><topic>Immunologic Memory - immunology</topic><topic>Immunological memory</topic><topic>Immunophenotyping</topic><topic>Immunotherapy</topic><topic>Infants</topic><topic>Infections</topic><topic>Interleukin 10</topic><topic>Interleukin 2</topic><topic>Interleukin 4</topic><topic>Interleukin 5</topic><topic>Lectins, C-Type - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rieber, Nikolaus</au><au>Graf, Anna</au><au>Hartl, Dominik</au><au>Urschel, Simon</au><au>Belohradsky, Bernd H</au><au>Liese, Johannes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acellular pertussis booster in adolescents induces Th1 and memory CD8+ T cell immune response</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-03-08</date><risdate>2011</risdate><volume>6</volume><issue>3</issue><spage>e17271</spage><pages>e17271-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In a number of countries, whole cell pertussis vaccines (wcP) were replaced by acellular vaccines (aP) due to an improved reactogenicity profile. Pertussis immunization leads to specific antibody production with the help of CD4(+) T cells. In earlier studies in infants and young children, wcP vaccines selectively induced a Th1 dominated immune response, whereas aP vaccines led to a Th2 biased response. To obtain data on Th1 or Th2 dominance of the immune response in adolescents receiving an aP booster immunization after a wcP or aP primary immunization, we analyzed the concentration of Th1 (IL-2, TNF-α, INF-γ) and Th2 (IL-4, IL-5, IL-10) cytokines in supernatants of lymphocyte cultures specifically stimulated with pertussis antigens. We also investigated the presence of cytotoxic T cell responses against the facultative intracellular bacterium Bordetella pertussis by quantifying pertussis-specific CD8(+) T cell activation following the aP booster immunization. Here we show that the adolescent aP booster vaccination predominantly leads to a Th1 immune response based on IFNgamma secretion upon stimulation with pertussis antigen, irrespective of a prior whole cell or acellular primary vaccination. The vaccination also induces an increase in peripheral CD8(+)CD69(+) activated pertussis-specific memory T cells four weeks after vaccination. The Th1 bias of this immune response could play a role for the decreased local reactogenicity of this adolescent aP booster immunization when compared to the preceding childhood acellular pertussis booster. Pertussis-specific CD8(+) memory T cells may contribute to protection against clinical pertussis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21408149</pmid><doi>10.1371/journal.pone.0017271</doi><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adolescents Age Aluminum Antigens Antigens, CD - immunology Antigens, Differentiation, T-Lymphocyte - immunology Biology Bordetella Bordetella pertussis CD4 antigen CD69 antigen CD8 antigen CD8-Positive T-Lymphocytes - immunology Cell activation Chicken pox Child Children Children & youth Clinical trials Cytokines Cytokines - immunology Cytotoxicity Demography Diphtheria Female Humans Immune response Immune response (cell-mediated) Immune system Immunization Immunization, Secondary Immunologic Memory - immunology Immunological memory Immunophenotyping Immunotherapy Infants Infections Interleukin 10 Interleukin 2 Interleukin 4 Interleukin 5 Lectins, C-Type - immunology Licensed products Localization Lymphocyte Activation - immunology Lymphocytes Lymphocytes T Male Medicine Memory cells Pertussis Pertussis Vaccine - immunology Studies Teenagers Tetanus Th1 Cells - immunology Th2 Cells - immunology Tumor necrosis factor-α Vaccination Vaccines Vaccines, Acellular - immunology Whooping cough Whooping Cough - immunology |
| title | Acellular pertussis booster in adolescents induces Th1 and memory CD8+ T cell immune response |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T02%3A05%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Acellular%20pertussis%20booster%20in%20adolescents%20induces%20Th1%20and%20memory%20CD8+%20T%20cell%20immune%20response&rft.jtitle=PloS%20one&rft.au=Rieber,%20Nikolaus&rft.date=2011-03-08&rft.volume=6&rft.issue=3&rft.spage=e17271&rft.pages=e17271-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0017271&rft_dat=%3Cproquest_plos_%3E907152430%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1295221273&rft_id=info:pmid/21408149&rft_doaj_id=oai_doaj_org_article_8710b737a3384d59af0b8e50450fdce6&rfr_iscdi=true |