Robust, Simple Genotyping-by-Sequencing (GBS) Approach for High Diversity Species

Advances in next generation technologies have driven the costs of DNA sequencing down to the point that genotyping-by-sequencing (GBS) is now feasible for high diversity, large genome species. Here, we report a procedure for constructing GBS libraries based on reducing genome complexity with restric...

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Veröffentlicht in:	PloS one 2011-05, Vol.6 (5), p.e19379-e19379
Hauptverfasser:	Elshire, Robert J, Glaubitz, Jeffrey C, Sun, Qi, Poland, Jesse A, Kawamoto, Ken, Buckler, Edward S, Mitchell, Sharon E, Orban, Laszlo
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Analysis Animal behavior Barley Binding sites Biodiversity biologists Biology Breeding Computing time Conservation Conserved sequence Corn Deoxyribonucleic acid DNA DNA methylation DNA sequencing Domestication Efficiency Enzymes Gene sequencing Genetic diversity Genetic testing genetic variation genome Genomes Genomics Genotype Genotyping Germplasm Haplotypes High-Throughput Nucleotide Sequencing - methods Hordeum - classification Hordeum - genetics Inbreeding kinship marker-assisted selection Methylation Nucleotide sequence nucleotide sequences Nutrition research plant breeders Plant breeding Polymerase Chain Reaction Polymorphism, Single Nucleotide Population Population structure restriction endonucleases sequence analysis Sequence Tagged Sites Species Species diversity surveys Tags Wildlife conservation Zea mays - classification Zea mays - genetics
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