Quantitative and qualitative urinary cellular patterns correlate with progression of murine glomerulonephritis
The kidney is a nonregenerative organ composed of numerous functional nephrons and collecting ducts (CDs). Glomerular and tubulointerstitial damages decrease the number of functional nephrons and cause anatomical and physiological alterations resulting in renal dysfunction. It has recently been repo...
Gespeichert in:
| Veröffentlicht in: | PloS one 2011-01, Vol.6 (1), p.e16472-e16472 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e16472 |
|---|---|
| container_issue | 1 |
| container_start_page | e16472 |
| container_title | PloS one |
| container_volume | 6 |
| creator | Kimura, Junpei Ichii, Osamu Otsuka, Saori Kanazawa, Tomonori Namiki, Yuka Hashimoto, Yoshiharu Kon, Yasuhiro |
| description | The kidney is a nonregenerative organ composed of numerous functional nephrons and collecting ducts (CDs). Glomerular and tubulointerstitial damages decrease the number of functional nephrons and cause anatomical and physiological alterations resulting in renal dysfunction. It has recently been reported that nephron constituent cells are dropped into the urine in several pathological conditions associated with renal functional deterioration. We investigated the quantitative and qualitative urinary cellular patterns in a murine glomerulonephritis model and elucidated the correlation between cellular patterns and renal pathology.Urinary cytology and renal histopathology were analyzed in BXSB/MpJ (BXSB; glomerulonephritis model) and C57BL/6 (B6; control) mice. Urinary cytology revealed that the number of urinary cells in BXSB mice changed according to the histometric score of glomerulonephritis and urinary albumin; however, no correlation was detected for the levels of blood urea nitrogen and creatinine. The expression of specific markers for podocytes, distal tubules (DTs), and CDs was detected in BXSB urine. Cells immunopositive for Wilms tumor 1 (podocyte marker) and interleukin-1 family, member 6 (damaged DT and CD marker) in the kidney significantly decreased and increased in BXSB versus B6, respectively. In the PCR array analysis of inflammatory cytokines and chemokines, Il10, Cxcl2, C3, and Il1rn showed relatively higher expression in BXSB kidneys than in B6 kidneys. In particular, the highest expression of C3 mRNA was detected in the urine from BXSB mice. Furthermore, C3 protein and mRNA were localized in the epithelia of damaged nephrons.These findings suggest that epithelial cells of the glomerulus, DT, and CD are dropped into the urine, and that these patterns are associated with renal pathology progression. We conclude that evaluation of urinary cellular patterns plays a key role in the early, noninvasive diagnosis of renal disease. |
| doi_str_mv | 10.1371/journal.pone.0016472 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1294199716</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A476906640</galeid><doaj_id>oai_doaj_org_article_f288def6411549ba9cd3150198d1eee4</doaj_id><sourcerecordid>A476906640</sourcerecordid><originalsourceid>FETCH-LOGICAL-c735t-2c1058b8547b15841f71005637b9a0c204736889ba0e3b18502a2fcb941eaad53</originalsourceid><addsrcrecordid>eNqNk1uL1DAUx4so7rr6DUQLguLDjLn1kpeFZfEysLB4fQ1petrJkEm6SbrqtzfjdJap7IPkoWny-__PyUlOlj3HaIlphd9t3OitNMvBWVgihEtWkQfZKeaULEqC6MOj-Un2JIQNQgWty_JxdkIwRYxzcprZz6O0UUcZ9S3k0rb5zSjN4X_02kr_O1dgzGikzwcZI3gbcuW8ByMj5D91XOeDd72HELSzuevy7U4IeW_cFvxoUobD2uuow9PsUSdNgGfT9yz7_uH9t8tPi6vrj6vLi6uFqmgRF0RhVNRNXbCqwUXNcFfhlH1Jq4ZLpAhiFS3rmjcSAW1wXSAiSacazjBI2Rb0LHu59x2MC2IqVRCYJILzCpeJWO2J1smNGLzepoMKJ7X4u-B8L6SPWhkQHanrFrqSYVywFJOrluICYV63GABY8jqfoo3NFloFNnppZqbzHavXone3gqJkxHEyeDMZeHczQohiq8Ou6NKCG4OoC0xIhQhJ5Kt_yPsPN1G9TPlr27kUVu08xQWrSo7KkqFELe-h0mhhq1W6tE6n9Zng7UyQmAi_Yi_HEMTq65f_Z69_zNnXR-wapInr4MwY03MKc5DtQeVdCB66uxpjJHZdcaiG2HWFmLoiyV4c38-d6NAG9A9wiwkz</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1294199716</pqid></control><display><type>article</type><title>Quantitative and qualitative urinary cellular patterns correlate with progression of murine glomerulonephritis</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Kimura, Junpei ; Ichii, Osamu ; Otsuka, Saori ; Kanazawa, Tomonori ; Namiki, Yuka ; Hashimoto, Yoshiharu ; Kon, Yasuhiro</creator><contributor>Brown, Justin</contributor><creatorcontrib>Kimura, Junpei ; Ichii, Osamu ; Otsuka, Saori ; Kanazawa, Tomonori ; Namiki, Yuka ; Hashimoto, Yoshiharu ; Kon, Yasuhiro ; Brown, Justin</creatorcontrib><description>The kidney is a nonregenerative organ composed of numerous functional nephrons and collecting ducts (CDs). Glomerular and tubulointerstitial damages decrease the number of functional nephrons and cause anatomical and physiological alterations resulting in renal dysfunction. It has recently been reported that nephron constituent cells are dropped into the urine in several pathological conditions associated with renal functional deterioration. We investigated the quantitative and qualitative urinary cellular patterns in a murine glomerulonephritis model and elucidated the correlation between cellular patterns and renal pathology.Urinary cytology and renal histopathology were analyzed in BXSB/MpJ (BXSB; glomerulonephritis model) and C57BL/6 (B6; control) mice. Urinary cytology revealed that the number of urinary cells in BXSB mice changed according to the histometric score of glomerulonephritis and urinary albumin; however, no correlation was detected for the levels of blood urea nitrogen and creatinine. The expression of specific markers for podocytes, distal tubules (DTs), and CDs was detected in BXSB urine. Cells immunopositive for Wilms tumor 1 (podocyte marker) and interleukin-1 family, member 6 (damaged DT and CD marker) in the kidney significantly decreased and increased in BXSB versus B6, respectively. In the PCR array analysis of inflammatory cytokines and chemokines, Il10, Cxcl2, C3, and Il1rn showed relatively higher expression in BXSB kidneys than in B6 kidneys. In particular, the highest expression of C3 mRNA was detected in the urine from BXSB mice. Furthermore, C3 protein and mRNA were localized in the epithelia of damaged nephrons.These findings suggest that epithelial cells of the glomerulus, DT, and CD are dropped into the urine, and that these patterns are associated with renal pathology progression. We conclude that evaluation of urinary cellular patterns plays a key role in the early, noninvasive diagnosis of renal disease.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0016472</identifier><identifier>PMID: 21304992</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Albumin ; Analysis ; Animals ; Autoimmune diseases ; Biomarkers - urine ; Biosynthesis ; C3 protein ; Cell Count ; Chemokines ; Correlation ; Creatinine ; Cytokines ; Cytology ; Damage localization ; Distal tubules ; Epithelial cells ; Epithelial Cells - pathology ; Fibroblasts ; Gene expression ; Glomerulonephritis ; Glomerulonephritis - diagnosis ; Glomerulonephritis - urine ; Glomerulus ; Histochemistry ; Histopathology ; House mouse ; Inflammation ; Inflammatory diseases ; Interleukin 1 ; Interleukin 10 ; Kidney diseases ; Kidney Diseases - diagnosis ; Kidney Tubules, Distal - pathology ; Kidneys ; Laboratory animals ; Medicine ; Mice ; Mice, Inbred C57BL ; mRNA ; Nephrons ; Pathology ; Physiological aspects ; Podocytes - pathology ; Proteins ; Qualitative analysis ; Renal function ; RNA ; Rodents ; Urea ; Urine ; Urine - cytology ; Veterinary colleges ; Veterinary medicine ; WT1 protein</subject><ispartof>PloS one, 2011-01, Vol.6 (1), p.e16472-e16472</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Kimura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Kimura et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c735t-2c1058b8547b15841f71005637b9a0c204736889ba0e3b18502a2fcb941eaad53</citedby><cites>FETCH-LOGICAL-c735t-2c1058b8547b15841f71005637b9a0c204736889ba0e3b18502a2fcb941eaad53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031591/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3031591/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21304992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Brown, Justin</contributor><creatorcontrib>Kimura, Junpei</creatorcontrib><creatorcontrib>Ichii, Osamu</creatorcontrib><creatorcontrib>Otsuka, Saori</creatorcontrib><creatorcontrib>Kanazawa, Tomonori</creatorcontrib><creatorcontrib>Namiki, Yuka</creatorcontrib><creatorcontrib>Hashimoto, Yoshiharu</creatorcontrib><creatorcontrib>Kon, Yasuhiro</creatorcontrib><title>Quantitative and qualitative urinary cellular patterns correlate with progression of murine glomerulonephritis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The kidney is a nonregenerative organ composed of numerous functional nephrons and collecting ducts (CDs). Glomerular and tubulointerstitial damages decrease the number of functional nephrons and cause anatomical and physiological alterations resulting in renal dysfunction. It has recently been reported that nephron constituent cells are dropped into the urine in several pathological conditions associated with renal functional deterioration. We investigated the quantitative and qualitative urinary cellular patterns in a murine glomerulonephritis model and elucidated the correlation between cellular patterns and renal pathology.Urinary cytology and renal histopathology were analyzed in BXSB/MpJ (BXSB; glomerulonephritis model) and C57BL/6 (B6; control) mice. Urinary cytology revealed that the number of urinary cells in BXSB mice changed according to the histometric score of glomerulonephritis and urinary albumin; however, no correlation was detected for the levels of blood urea nitrogen and creatinine. The expression of specific markers for podocytes, distal tubules (DTs), and CDs was detected in BXSB urine. Cells immunopositive for Wilms tumor 1 (podocyte marker) and interleukin-1 family, member 6 (damaged DT and CD marker) in the kidney significantly decreased and increased in BXSB versus B6, respectively. In the PCR array analysis of inflammatory cytokines and chemokines, Il10, Cxcl2, C3, and Il1rn showed relatively higher expression in BXSB kidneys than in B6 kidneys. In particular, the highest expression of C3 mRNA was detected in the urine from BXSB mice. Furthermore, C3 protein and mRNA were localized in the epithelia of damaged nephrons.These findings suggest that epithelial cells of the glomerulus, DT, and CD are dropped into the urine, and that these patterns are associated with renal pathology progression. We conclude that evaluation of urinary cellular patterns plays a key role in the early, noninvasive diagnosis of renal disease.</description><subject>Albumin</subject><subject>Analysis</subject><subject>Animals</subject><subject>Autoimmune diseases</subject><subject>Biomarkers - urine</subject><subject>Biosynthesis</subject><subject>C3 protein</subject><subject>Cell Count</subject><subject>Chemokines</subject><subject>Correlation</subject><subject>Creatinine</subject><subject>Cytokines</subject><subject>Cytology</subject><subject>Damage localization</subject><subject>Distal tubules</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - pathology</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Glomerulonephritis</subject><subject>Glomerulonephritis - diagnosis</subject><subject>Glomerulonephritis - urine</subject><subject>Glomerulus</subject><subject>Histochemistry</subject><subject>Histopathology</subject><subject>House mouse</subject><subject>Inflammation</subject><subject>Inflammatory diseases</subject><subject>Interleukin 1</subject><subject>Interleukin 10</subject><subject>Kidney diseases</subject><subject>Kidney Diseases - diagnosis</subject><subject>Kidney Tubules, Distal - pathology</subject><subject>Kidneys</subject><subject>Laboratory animals</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>mRNA</subject><subject>Nephrons</subject><subject>Pathology</subject><subject>Physiological aspects</subject><subject>Podocytes - pathology</subject><subject>Proteins</subject><subject>Qualitative analysis</subject><subject>Renal function</subject><subject>RNA</subject><subject>Rodents</subject><subject>Urea</subject><subject>Urine</subject><subject>Urine - cytology</subject><subject>Veterinary colleges</subject><subject>Veterinary medicine</subject><subject>WT1 protein</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1uL1DAUx4so7rr6DUQLguLDjLn1kpeFZfEysLB4fQ1petrJkEm6SbrqtzfjdJap7IPkoWny-__PyUlOlj3HaIlphd9t3OitNMvBWVgihEtWkQfZKeaULEqC6MOj-Un2JIQNQgWty_JxdkIwRYxzcprZz6O0UUcZ9S3k0rb5zSjN4X_02kr_O1dgzGikzwcZI3gbcuW8ByMj5D91XOeDd72HELSzuevy7U4IeW_cFvxoUobD2uuow9PsUSdNgGfT9yz7_uH9t8tPi6vrj6vLi6uFqmgRF0RhVNRNXbCqwUXNcFfhlH1Jq4ZLpAhiFS3rmjcSAW1wXSAiSacazjBI2Rb0LHu59x2MC2IqVRCYJILzCpeJWO2J1smNGLzepoMKJ7X4u-B8L6SPWhkQHanrFrqSYVywFJOrluICYV63GABY8jqfoo3NFloFNnppZqbzHavXone3gqJkxHEyeDMZeHczQohiq8Ou6NKCG4OoC0xIhQhJ5Kt_yPsPN1G9TPlr27kUVu08xQWrSo7KkqFELe-h0mhhq1W6tE6n9Zng7UyQmAi_Yi_HEMTq65f_Z69_zNnXR-wapInr4MwY03MKc5DtQeVdCB66uxpjJHZdcaiG2HWFmLoiyV4c38-d6NAG9A9wiwkz</recordid><startdate>20110131</startdate><enddate>20110131</enddate><creator>Kimura, Junpei</creator><creator>Ichii, Osamu</creator><creator>Otsuka, Saori</creator><creator>Kanazawa, Tomonori</creator><creator>Namiki, Yuka</creator><creator>Hashimoto, Yoshiharu</creator><creator>Kon, Yasuhiro</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110131</creationdate><title>Quantitative and qualitative urinary cellular patterns correlate with progression of murine glomerulonephritis</title><author>Kimura, Junpei ; Ichii, Osamu ; Otsuka, Saori ; Kanazawa, Tomonori ; Namiki, Yuka ; Hashimoto, Yoshiharu ; Kon, Yasuhiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c735t-2c1058b8547b15841f71005637b9a0c204736889ba0e3b18502a2fcb941eaad53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Albumin</topic><topic>Analysis</topic><topic>Animals</topic><topic>Autoimmune diseases</topic><topic>Biomarkers - urine</topic><topic>Biosynthesis</topic><topic>C3 protein</topic><topic>Cell Count</topic><topic>Chemokines</topic><topic>Correlation</topic><topic>Creatinine</topic><topic>Cytokines</topic><topic>Cytology</topic><topic>Damage localization</topic><topic>Distal tubules</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - pathology</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>Glomerulonephritis</topic><topic>Glomerulonephritis - diagnosis</topic><topic>Glomerulonephritis - urine</topic><topic>Glomerulus</topic><topic>Histochemistry</topic><topic>Histopathology</topic><topic>House mouse</topic><topic>Inflammation</topic><topic>Inflammatory diseases</topic><topic>Interleukin 1</topic><topic>Interleukin 10</topic><topic>Kidney diseases</topic><topic>Kidney Diseases - diagnosis</topic><topic>Kidney Tubules, Distal - pathology</topic><topic>Kidneys</topic><topic>Laboratory animals</topic><topic>Medicine</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>mRNA</topic><topic>Nephrons</topic><topic>Pathology</topic><topic>Physiological aspects</topic><topic>Podocytes - pathology</topic><topic>Proteins</topic><topic>Qualitative analysis</topic><topic>Renal function</topic><topic>RNA</topic><topic>Rodents</topic><topic>Urea</topic><topic>Urine</topic><topic>Urine - cytology</topic><topic>Veterinary colleges</topic><topic>Veterinary medicine</topic><topic>WT1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimura, Junpei</creatorcontrib><creatorcontrib>Ichii, Osamu</creatorcontrib><creatorcontrib>Otsuka, Saori</creatorcontrib><creatorcontrib>Kanazawa, Tomonori</creatorcontrib><creatorcontrib>Namiki, Yuka</creatorcontrib><creatorcontrib>Hashimoto, Yoshiharu</creatorcontrib><creatorcontrib>Kon, Yasuhiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimura, Junpei</au><au>Ichii, Osamu</au><au>Otsuka, Saori</au><au>Kanazawa, Tomonori</au><au>Namiki, Yuka</au><au>Hashimoto, Yoshiharu</au><au>Kon, Yasuhiro</au><au>Brown, Justin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative and qualitative urinary cellular patterns correlate with progression of murine glomerulonephritis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-01-31</date><risdate>2011</risdate><volume>6</volume><issue>1</issue><spage>e16472</spage><epage>e16472</epage><pages>e16472-e16472</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The kidney is a nonregenerative organ composed of numerous functional nephrons and collecting ducts (CDs). Glomerular and tubulointerstitial damages decrease the number of functional nephrons and cause anatomical and physiological alterations resulting in renal dysfunction. It has recently been reported that nephron constituent cells are dropped into the urine in several pathological conditions associated with renal functional deterioration. We investigated the quantitative and qualitative urinary cellular patterns in a murine glomerulonephritis model and elucidated the correlation between cellular patterns and renal pathology.Urinary cytology and renal histopathology were analyzed in BXSB/MpJ (BXSB; glomerulonephritis model) and C57BL/6 (B6; control) mice. Urinary cytology revealed that the number of urinary cells in BXSB mice changed according to the histometric score of glomerulonephritis and urinary albumin; however, no correlation was detected for the levels of blood urea nitrogen and creatinine. The expression of specific markers for podocytes, distal tubules (DTs), and CDs was detected in BXSB urine. Cells immunopositive for Wilms tumor 1 (podocyte marker) and interleukin-1 family, member 6 (damaged DT and CD marker) in the kidney significantly decreased and increased in BXSB versus B6, respectively. In the PCR array analysis of inflammatory cytokines and chemokines, Il10, Cxcl2, C3, and Il1rn showed relatively higher expression in BXSB kidneys than in B6 kidneys. In particular, the highest expression of C3 mRNA was detected in the urine from BXSB mice. Furthermore, C3 protein and mRNA were localized in the epithelia of damaged nephrons.These findings suggest that epithelial cells of the glomerulus, DT, and CD are dropped into the urine, and that these patterns are associated with renal pathology progression. We conclude that evaluation of urinary cellular patterns plays a key role in the early, noninvasive diagnosis of renal disease.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21304992</pmid><doi>10.1371/journal.pone.0016472</doi><tpages>e16472</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2011-01, Vol.6 (1), p.e16472-e16472 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1294199716 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Albumin Analysis Animals Autoimmune diseases Biomarkers - urine Biosynthesis C3 protein Cell Count Chemokines Correlation Creatinine Cytokines Cytology Damage localization Distal tubules Epithelial cells Epithelial Cells - pathology Fibroblasts Gene expression Glomerulonephritis Glomerulonephritis - diagnosis Glomerulonephritis - urine Glomerulus Histochemistry Histopathology House mouse Inflammation Inflammatory diseases Interleukin 1 Interleukin 10 Kidney diseases Kidney Diseases - diagnosis Kidney Tubules, Distal - pathology Kidneys Laboratory animals Medicine Mice Mice, Inbred C57BL mRNA Nephrons Pathology Physiological aspects Podocytes - pathology Proteins Qualitative analysis Renal function RNA Rodents Urea Urine Urine - cytology Veterinary colleges Veterinary medicine WT1 protein |
| title | Quantitative and qualitative urinary cellular patterns correlate with progression of murine glomerulonephritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T17%3A45%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantitative%20and%20qualitative%20urinary%20cellular%20patterns%20correlate%20with%20progression%20of%20murine%20glomerulonephritis&rft.jtitle=PloS%20one&rft.au=Kimura,%20Junpei&rft.date=2011-01-31&rft.volume=6&rft.issue=1&rft.spage=e16472&rft.epage=e16472&rft.pages=e16472-e16472&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0016472&rft_dat=%3Cgale_plos_%3EA476906640%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1294199716&rft_id=info:pmid/21304992&rft_galeid=A476906640&rft_doaj_id=oai_doaj_org_article_f288def6411549ba9cd3150198d1eee4&rfr_iscdi=true |