Superoxide flashes in mouse skeletal muscle are produced by discrete arrays of active mitochondria operating coherently

Reactive Oxygen Species (ROS) constitute important intracellular signaling molecules. Mitochondria are admitted sources of ROS, especially of superoxide anions through the electron transport chain. Here the mitochondria-targeted ratiometric pericam (RPmt) was used as a superoxide biosensor, by appro...

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description Reactive Oxygen Species (ROS) constitute important intracellular signaling molecules. Mitochondria are admitted sources of ROS, especially of superoxide anions through the electron transport chain. Here the mitochondria-targeted ratiometric pericam (RPmt) was used as a superoxide biosensor, by appropriate choice of the excitation wavelength. RPmt was transfected in vivo into mouse muscles. Confocal imaging of isolated muscle fibers reveals spontaneous flashes of RPmt fluorescence. Flashes correspond to increases in superoxide production, as shown by simultaneous recordings of the fluorescence from MitoSox, a mitochondrial superoxide probe. Flashes occur in all subcellular populations of mitochondria. Spatial analysis of the flashes pattern over time revealed that arrays of mitochondria work as well-defined superoxide-production-units. Increase of superoxide production at the muscle fiber level involves recruitment of supplemental units with no increase in per-unit production. Altogether, these results demonstrate that superoxide flashes in muscle fibers correspond to physiological signals linked to mitochondrial metabolism. They also suggest that superoxide, or one of its derivatives, modulates its own production at the mitochondrial level.
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Mitochondria are admitted sources of ROS, especially of superoxide anions through the electron transport chain. Here the mitochondria-targeted ratiometric pericam (RPmt) was used as a superoxide biosensor, by appropriate choice of the excitation wavelength. RPmt was transfected in vivo into mouse muscles. Confocal imaging of isolated muscle fibers reveals spontaneous flashes of RPmt fluorescence. Flashes correspond to increases in superoxide production, as shown by simultaneous recordings of the fluorescence from MitoSox, a mitochondrial superoxide probe. Flashes occur in all subcellular populations of mitochondria. Spatial analysis of the flashes pattern over time revealed that arrays of mitochondria work as well-defined superoxide-production-units. Increase of superoxide production at the muscle fiber level involves recruitment of supplemental units with no increase in per-unit production. Altogether, these results demonstrate that superoxide flashes in muscle fibers correspond to physiological signals linked to mitochondrial metabolism. They also suggest that superoxide, or one of its derivatives, modulates its own production at the mitochondrial level.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20927399</pmid><doi>10.1371/journal.pone.0013035</doi><tpages>e13035</tpages><oa>free_for_read</oa></addata></record>
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subjects Animals
Anions
Biosensors
Cardiomyocytes
Cell Biology/Cell Signaling
Confocal
Electron transport
Electron transport chain
Fluorescence
Gene expression
Intracellular signalling
Male
Metabolism
Mice
Microscopy, Confocal
Mitochondria
Mitochondria, Muscle - chemistry
Mitochondria, Muscle - metabolism
Muscle, Skeletal - chemistry
Muscle, Skeletal - metabolism
Muscles
Musculoskeletal system
Oxygen
Permeability
Physiological aspects
Physiology
Physiology/Cell Signaling
Physiology/Muscle and Connective Tissue
Protein expression
Proteins
Reactive oxygen species
Recruitment
Skeletal muscle
Spatial analysis
Superoxide
Superoxide anions
Superoxides
Superoxides - analysis
Superoxides - metabolism
Trends
title Superoxide flashes in mouse skeletal muscle are produced by discrete arrays of active mitochondria operating coherently
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