The Pseudomonas aeruginosa chemotaxis methyltransferase CheR1 impacts on bacterial surface sampling
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that...
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| Veröffentlicht in: | PloS one 2011-03, Vol.6 (3), p.e18184-e18184 |
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| creator | Schmidt, Juliane Müsken, Mathias Becker, Tanja Magnowska, Zofia Bertinetti, Daniela Möller, Stefan Zimmermann, Bastian Herberg, Friedrich W Jänsch, Lothar Häussler, Susanne |
| description | The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa. |
| doi_str_mv | 10.1371/journal.pone.0018184 |
| format | Article |
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Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0018184</identifier><identifier>PMID: 21445368</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenosylmethionine ; Amino acids ; Attractants ; Bacteria ; Bacterial infections ; Biochemistry ; Biofilms ; Biology ; Chemoreception ; Chemoreceptors ; Chemotaxis ; Colonization ; E coli ; Escherichia ; Escherichia coli ; Flagella ; Genes ; Genetic aspects ; Genetic engineering ; Glutamine ; Infections ; Joint ventures ; Methyltransferase ; Methyltransferases - genetics ; Methyltransferases - metabolism ; Motility ; Mutagenesis ; Mutation ; Protein binding ; Proteins ; Pseudomonas ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - enzymology ; Pseudomonas aeruginosa - isolation & purification ; Pseudomonas aeruginosa - physiology ; Pseudomonas fluorescens ; S-Adenosylmethionine ; Sampling ; Serine ; Signal transduction ; Swarming ; Swimming ; Transferases ; Twitching</subject><ispartof>PloS one, 2011-03, Vol.6 (3), p.e18184-e18184</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Schmidt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa.</description><subject>Adenosylmethionine</subject><subject>Amino acids</subject><subject>Attractants</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Biochemistry</subject><subject>Biofilms</subject><subject>Biology</subject><subject>Chemoreception</subject><subject>Chemoreceptors</subject><subject>Chemotaxis</subject><subject>Colonization</subject><subject>E coli</subject><subject>Escherichia</subject><subject>Escherichia coli</subject><subject>Flagella</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic engineering</subject><subject>Glutamine</subject><subject>Infections</subject><subject>Joint ventures</subject><subject>Methyltransferase</subject><subject>Methyltransferases - genetics</subject><subject>Methyltransferases 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Pseudomonas aeruginosa chemotaxis methyltransferase CheR1 impacts on bacterial surface sampling</title><author>Schmidt, Juliane ; Müsken, Mathias ; Becker, Tanja ; Magnowska, Zofia ; Bertinetti, Daniela ; Möller, Stefan ; Zimmermann, Bastian ; Herberg, Friedrich W ; Jänsch, Lothar ; Häussler, Susanne</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c723t-c75df11813c1de3015a5633a5176a5d0e58adf7687085f7b9ed441844a4e90d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenosylmethionine</topic><topic>Amino acids</topic><topic>Attractants</topic><topic>Bacteria</topic><topic>Bacterial infections</topic><topic>Biochemistry</topic><topic>Biofilms</topic><topic>Biology</topic><topic>Chemoreception</topic><topic>Chemoreceptors</topic><topic>Chemotaxis</topic><topic>Colonization</topic><topic>E coli</topic><topic>Escherichia</topic><topic>Escherichia 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Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. 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| subjects | Adenosylmethionine Amino acids Attractants Bacteria Bacterial infections Biochemistry Biofilms Biology Chemoreception Chemoreceptors Chemotaxis Colonization E coli Escherichia Escherichia coli Flagella Genes Genetic aspects Genetic engineering Glutamine Infections Joint ventures Methyltransferase Methyltransferases - genetics Methyltransferases - metabolism Motility Mutagenesis Mutation Protein binding Proteins Pseudomonas Pseudomonas aeruginosa Pseudomonas aeruginosa - enzymology Pseudomonas aeruginosa - isolation & purification Pseudomonas aeruginosa - physiology Pseudomonas fluorescens S-Adenosylmethionine Sampling Serine Signal transduction Swarming Swimming Transferases Twitching |
| title | The Pseudomonas aeruginosa chemotaxis methyltransferase CheR1 impacts on bacterial surface sampling |
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