Modulation of human immune responses by bovine interleukin-10
Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood mon...
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| Veröffentlicht in: | PloS one 2011-03, Vol.6 (3), p.e18188 |
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| creator | den Hartog, Gerco Savelkoul, Huub F J Schoemaker, Ruud Tijhaar, Edwin Westphal, Adrie H de Ruiter, Talitha van de Weg-Schrijver, Elise van Neerven, R J Joost |
| description | Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets. |
| doi_str_mv | 10.1371/journal.pone.0018188 |
| format | Article |
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Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0018188</identifier><identifier>PMID: 21464967</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Activation ; Allergies ; Amino Acid Sequence ; Amino Acid Substitution - genetics ; Amino acids ; Animals ; Antibodies ; B cells ; Binding Sites ; Biological Availability ; Biology ; Biomarkers - metabolism ; Blocking antibodies ; Breastfeeding & lactation ; Cattle ; Cell activation ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; cytokine production ; Cytokines ; Dairy products ; Dendritic cells ; Dendritic Cells - cytology ; Dendritic Cells - drug effects ; Dendritic Cells - metabolism ; Enterocolitis ; Food ; Granulocyte-macrophage colony-stimulating factor ; Growth factors ; Human behavior ; human-milk ; Humans ; Hypersensitivity ; Hypoxia ; ifn-gamma ; Immune response ; Immunity - drug effects ; Immunological tolerance ; Immunology ; Immunomodulation ; Inhibition ; interferon-gamma ; Interleukin ; Interleukin 10 ; Interleukin 4 ; Interleukin-10 - chemistry ; Interleukin-10 - pharmacology ; Interleukins ; Intestine ; Ligands ; Lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Lymphocytes ; Medicine ; Milk ; Molecular Sequence Data ; Monocytes ; Monocytes - cytology ; Monocytes - drug effects ; Monocytes - metabolism ; Mucosa ; Myeloid cells ; Necrotizing enterocolitis ; Pediatrics ; Peripheral blood ; Receptors, Interleukin-10 - chemistry ; Receptors, Interleukin-10 - metabolism ; Rodents ; sequence identity ; sublingual immunotherapy ; t-cell proliferation ; tgf-beta ; Toll-Like Receptors - metabolism</subject><ispartof>PloS one, 2011-03, Vol.6 (3), p.e18188</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 den Hartog et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>den Hartog et al. 2011</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c742t-2ce5df6b0b8e51f45ba2524caa797a02c778d72d0afaaa7d43b5d11a58b837483</citedby><cites>FETCH-LOGICAL-c742t-2ce5df6b0b8e51f45ba2524caa797a02c778d72d0afaaa7d43b5d11a58b837483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064667/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064667/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23864,27922,27923,53789,53791,79370,79371</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21464967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Huygen, Kris</contributor><creatorcontrib>den Hartog, Gerco</creatorcontrib><creatorcontrib>Savelkoul, Huub F J</creatorcontrib><creatorcontrib>Schoemaker, Ruud</creatorcontrib><creatorcontrib>Tijhaar, Edwin</creatorcontrib><creatorcontrib>Westphal, Adrie H</creatorcontrib><creatorcontrib>de Ruiter, Talitha</creatorcontrib><creatorcontrib>van de Weg-Schrijver, Elise</creatorcontrib><creatorcontrib>van Neerven, R J Joost</creatorcontrib><title>Modulation of human immune responses by bovine interleukin-10</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. 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genetics</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>B cells</topic><topic>Binding Sites</topic><topic>Biological Availability</topic><topic>Biology</topic><topic>Biomarkers - metabolism</topic><topic>Blocking antibodies</topic><topic>Breastfeeding & lactation</topic><topic>Cattle</topic><topic>Cell activation</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>cytokine production</topic><topic>Cytokines</topic><topic>Dairy products</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - metabolism</topic><topic>Enterocolitis</topic><topic>Food</topic><topic>Granulocyte-macrophage colony-stimulating factor</topic><topic>Growth factors</topic><topic>Human behavior</topic><topic>human-milk</topic><topic>Humans</topic><topic>Hypersensitivity</topic><topic>Hypoxia</topic><topic>ifn-gamma</topic><topic>Immune response</topic><topic>Immunity - 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Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21464967</pmid><doi>10.1371/journal.pone.0018188</doi><tpages>e18188</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1292619951 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed (Medline); Public Library of Science; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Activation Allergies Amino Acid Sequence Amino Acid Substitution - genetics Amino acids Animals Antibodies B cells Binding Sites Biological Availability Biology Biomarkers - metabolism Blocking antibodies Breastfeeding & lactation Cattle Cell activation Cell Membrane - drug effects Cell Membrane - metabolism cytokine production Cytokines Dairy products Dendritic cells Dendritic Cells - cytology Dendritic Cells - drug effects Dendritic Cells - metabolism Enterocolitis Food Granulocyte-macrophage colony-stimulating factor Growth factors Human behavior human-milk Humans Hypersensitivity Hypoxia ifn-gamma Immune response Immunity - drug effects Immunological tolerance Immunology Immunomodulation Inhibition interferon-gamma Interleukin Interleukin 10 Interleukin 4 Interleukin-10 - chemistry Interleukin-10 - pharmacology Interleukins Intestine Ligands Lipopolysaccharides Lipopolysaccharides - pharmacology Lymphocytes Medicine Milk Molecular Sequence Data Monocytes Monocytes - cytology Monocytes - drug effects Monocytes - metabolism Mucosa Myeloid cells Necrotizing enterocolitis Pediatrics Peripheral blood Receptors, Interleukin-10 - chemistry Receptors, Interleukin-10 - metabolism Rodents sequence identity sublingual immunotherapy t-cell proliferation tgf-beta Toll-Like Receptors - metabolism |
| title | Modulation of human immune responses by bovine interleukin-10 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T16%3A48%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modulation%20of%20human%20immune%20responses%20by%20bovine%20interleukin-10&rft.jtitle=PloS%20one&rft.au=den%20Hartog,%20Gerco&rft.date=2011-03-25&rft.volume=6&rft.issue=3&rft.spage=e18188&rft.pages=e18188-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0018188&rft_dat=%3Cgale_plos_%3EA476899537%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1292619951&rft_id=info:pmid/21464967&rft_galeid=A476899537&rft_doaj_id=oai_doaj_org_article_ba36882c9a20402f9b561788c72c2b7c&rfr_iscdi=true |